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The map label for this gene is pcaK [H]

Identifier: 157159067

GI number: 157159067

Start: 2416486

End: 2417844

Strand: Reverse

Name: pcaK [H]

Synonym: EcE24377A_2434

Alternate gene names: 157159067

Gene position: 2417844-2416486 (Counterclockwise)

Preceding gene: 157158394

Following gene: 157155116

Centisome position: 48.55

GC content: 57.25

Gene sequence:

>1359_bases
ATGACTCAACGACGTGATTTACAAGCACTGATCGATGCTGCTCCGGTCGGCAAGATGCAGTGGCGCGTTATCATCTGCTG
TTTTCTGGTGGTAATGCTCGATGGTTTTGACACCGCGGCTATCGGCTTTATCGCCCCTGATATTCGCACTCACTGGCAAC
TCACCGCAGGCGATCTCGCCCCGCTATTCGGCGCGGGTCTGCTGGGGTTAACCGCAGGCGCACTGCTTTGTGGCCCGTTG
TCCGATCGCTTTGGTCGCAAACGGGTGATTGAGCTGTGCGTCTTTTTGTTTGGCGCGTTAAGCCTTGCCTCGGCTTTCTC
ACCGGATCTGCAAACGCTGGTATTCCTGCGTTTCCTAACCGGCCTTGGGCTGGGTGGCGCGATGCCAAACACCATCACCA
TAACCTCGGAATATCTGCCTGCCCGCCGCCGCGGCGCGCTGGTGACGCTGATGTTTTGCGGCTTTACGCTGGGTTCGGCG
TTTGGCGGGATTGTTAGCGCGCAGCTGGTGCCGGTGATTGGCTGGCACGGCATTCTGGTGTTGGGGGGCGTGTTACCGCT
GATGCTGTTTGTCGCACTGCTGGTAGTTCTGCCGGAATCGCCACGCTGGCAGGTGCGCCGCCAGCTACCGCAGGCGGTGA
TTGCGAAAACCGTCAGCGCCATCACCCGCGAGCGTTATGTCGATACCCACTTTTATCTCATTGAATCAGCCTCCGTTACA
AAAGGCAGCATTCGTCAACTGTTTATGGGACGTCAGTTACCCATCACGTTGATGCTGTGGGTAGTGTTCTTTATGAGCCT
GCTGATTATTTACCTGCTCTCAAGCTGGATGCCGACGCTATTGAACCATCGCGGCATCGACCTGCAACACGCGTCCTGGG
TCACCGCCGCGTTCCAGATTGGTGGTACGTTAGGCGCGCTGGCGCTGGGTGTGCTGATGGATAAGTTCAATCCTTTCCGC
GTACTGGCGCTGAGCTATGCCATAGGCGCTGTATGCATCGTGATGATTGGCTTAAGCCAGGACGGATTATGGCTGATGGC
GCTGGCGATTTTCGGCACCGGCATTGGTATTAGCGGATCGCAGGTGGGGCTGAATGCGCTGACCGCCACACTTTATCCAA
CACAAAGTCGCGCCACGGGCGTGAGCTGGTCAAACGCCGTGGGTCGCTGCGGTGCGATTGTTGGCTCGCTTTCTGGCGGC
GTGATGATGGCGATGAATTTCTCTTTCGACACCCTGTTTTTCATTATCGCTGTGCCCGCGGCGATCAGTGCGGTGATGTT
AGCCTTGCTGATTACCGTGGTTCGCCAGTCGACCTCTGTCCCTGACTCACTGCCTCGCGCGGGCGTTGTGAACGAATAA

Upstream 100 bases:

>100_bases
TGCTCGAAATGCCATTGTACATTTCATCTCTTTAATCACAATATCGCAACATACATAAAACAAAAGAAACATCTACCTTA
CCTGCACTGAGGCATTTACT

Downstream 100 bases:

>100_bases
GGAGCATCCTAATGACTGATAACAATCAAAATAGCCGCGAACAATTTTATCAGCACATTTCCGGGCAAAACCTGACCCCG
CTGTGGGAGTCACTACACCA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFIAPDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPL
SDRFGRKRVIELCVFLFGALSLASAFSPDLQTLVFLRFLTGLGLGGAMPNTITITSEYLPARRRGALVTLMFCGFTLGSA
FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTVSAITRERYVDTHFYLIESASVT
KGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSWMPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFR
VLALSYAIGAVCIVMIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAVGRCGAIVGSLSGG
VMMAMNFSFDTLFFIIAVPAAISAVMLALLITVVRQSTSVPDSLPRAGVVNE

Sequences:

>Translated_452_residues
MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFIAPDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPL
SDRFGRKRVIELCVFLFGALSLASAFSPDLQTLVFLRFLTGLGLGGAMPNTITITSEYLPARRRGALVTLMFCGFTLGSA
FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTVSAITRERYVDTHFYLIESASVT
KGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSWMPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFR
VLALSYAIGAVCIVMIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAVGRCGAIVGSLSGG
VMMAMNFSFDTLFFIIAVPAAISAVMLALLITVVRQSTSVPDSLPRAGVVNE
>Mature_451_residues
TQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFIAPDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLS
DRFGRKRVIELCVFLFGALSLASAFSPDLQTLVFLRFLTGLGLGGAMPNTITITSEYLPARRRGALVTLMFCGFTLGSAF
GGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTVSAITRERYVDTHFYLIESASVTK
GSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSWMPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRV
LALSYAIGAVCIVMIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAVGRCGAIVGSLSGGV
MMAMNFSFDTLFFIIAVPAAISAVMLALLITVVRQSTSVPDSLPRAGVVNE

Specific function: Could Be A Transporter For 3-Phenylpropionate (Hydrocinnamic Acid). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=422, Percent_Identity=26.303317535545, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI213021148, Length=451, Percent_Identity=26.3858093126386, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI7662270, Length=328, Percent_Identity=24.6951219512195, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI216548223, Length=332, Percent_Identity=24.3975903614458, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI166064021, Length=340, Percent_Identity=26.7647058823529, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI262399383, Length=265, Percent_Identity=26.0377358490566, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI4507005, Length=380, Percent_Identity=25.2631578947368, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI73695465, Length=336, Percent_Identity=24.4047619047619, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI24497476, Length=393, Percent_Identity=25.9541984732824, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI20070188, Length=393, Percent_Identity=25.9541984732824, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4506999, Length=335, Percent_Identity=26.5671641791045, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI87081723, Length=420, Percent_Identity=36.1904761904762, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1788068, Length=461, Percent_Identity=25.1626898047722, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87082231, Length=420, Percent_Identity=23.3333333333333, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI87082159, Length=396, Percent_Identity=28.2828282828283, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786229, Length=368, Percent_Identity=24.1847826086957, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1788074, Length=351, Percent_Identity=23.9316239316239, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1789152, Length=450, Percent_Identity=22, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI87082404, Length=383, Percent_Identity=21.4099216710183, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789207, Length=178, Percent_Identity=30.3370786516854, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=406, Percent_Identity=23.8916256157635, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320595, Length=141, Percent_Identity=30.4964539007092, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19922874, Length=407, Percent_Identity=23.5872235872236, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24640198, Length=460, Percent_Identity=24.1304347826087, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24640196, Length=460, Percent_Identity=24.1304347826087, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24640200, Length=460, Percent_Identity=24.1304347826087, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI161077756, Length=458, Percent_Identity=22.0524017467249, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48354; Mature: 48223

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFIAPDIRTHWQLTAGDLA
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHCEEECCCCHH
PLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSLASAFSPDLQTLVFLRFLT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GLGLGGAMPNTITITSEYLPARRRGALVTLMFCGFTLGSAFGGIVSAQLVPVIGWHGILV
HCCCCCCCCCEEEEEHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTVSAITRERYVDTHFYLIESASVT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
KGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSWMPTLLNHRGIDLQHASWVTAAFQI
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
GGTLGALALGVLMDKFNPFRVLALSYAIGAVCIVMIGLSQDGLWLMALAIFGTGIGISGS
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCC
QVGLNALTATLYPTQSRATGVSWSNAVGRCGAIVGSLSGGVMMAMNFSFDTLFFIIAVPA
CCCHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHH
AISAVMLALLITVVRQSTSVPDSLPRAGVVNE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFIAPDIRTHWQLTAGDLA
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHCEEECCCCHH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HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8063101 [H]