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The map label for this gene is dnaB [H]

Identifier: 157156830

GI number: 157156830

Start: 2212397

End: 2213788

Strand: Reverse

Name: dnaB [H]

Synonym: EcE24377A_2248

Alternate gene names: 157156830

Gene position: 2213788-2212397 (Counterclockwise)

Preceding gene: 157156443

Following gene: 157159115

Centisome position: 44.46

GC content: 52.3

Gene sequence:

>1392_bases
ATGAATCCAGAAGAACTGGAAGCGATCGTTCTCGCTGGTCTGATTAACGGTGGCGCGACGCCTGACGCGTTTGATGTGAT
TGCCACTACTCCGGAGGAAGCCTTCAGCATCGTGTTTCACCGTCGCGCTTTCAGCGAAATCAAAAAGCAGGCACTGGCGA
ACGGCATGATCGACATGTTGTTTATCAGCGAAGCGCTGGGCGGTTCCAGCCTTGCGGATCTGTCTCAAATATCCCGGATC
CCGGCAACGGTACCGAATCTGAAAGGCTATGCCGGGAAAATGGTGCGGGCATGGCGGAGCCGCAAAATGGCGGCATTGTT
GCAATCTGGCGCGGATGGTATCCGTAACGCTGTAAACCAGAGTCAGCGTGATGAGGTTATTGAATCTCATGTTGCTCAGG
TGCTGGATATGAGCGCCGCTACCGGCACAGTCCAGCCGGTACACATCAAAGAGCTGTTACCGCTCTACATTGACACCGTT
GATAAACGGATGTCTGGTGACGCGCAGGAAATGATGCTGAAAACCGGAGTCGAGGATCTGGATGTCGCCCTGGGCGGTAT
CAACATGACCGACCTTGTTGTGGTTGCCGGTCGTCCAGGCATGGGGAAAACGGAATTTACCCTGAAGATTATCGATGGCG
TTACCGCAGATGGTGGCGGCGCACTGTTTTTCAGCATGGAAATGGGCGCTCAGCAGATTGTTGAACGTACCGTTGCCGGG
GCTGGGAATATGTCCTCATCGCGTCTGCGTAATCCAAAAGAAATGGACGATGAGGACTGGTCTCGATTAACCGCCGCGCT
TGCAATCATGCAGGATCGCGATATCTGGATTGTTGATGCCACTGATTTGACGATTGAGCAGATCCGCGCCATTGCAGAGA
CTCATAAACGCCGTTATCCGCACCTGAAGCTGATTGGTGTTGATTACATGGGCCTGATCAAAAAACCGAAGGCCGAGCGC
CACGATCTCGCTGTGGGGCATATCTCCCGCAACATGAAGACTATGGCCATGCGTCTGCATACCCCTGTTTTTGCTCTTAG
CCAGCTTTCCCGCCAGGTGGATTCTCGACCTGCTGGCCAGCGTCGCCCGGTGATGTCGGACCTGCGCGATTCCGGCAGTG
TTGAGCAGGATGCTGACAGCATTTTGTTTTTGTATCGCGATGACGTTTACACACCTGATTCACCTGCAAAGGGTATCGCT
GAGGTGATTATTGGTAAAAACCGTTCTGGCGGGGCAGGTGAAATTATTTATCAGGAATTCAGGAATGGGCATTTCCTGCC
AGTTGATCAACACGTAGCGCGCGAAAAAATACGCATCCAGAAGGAGGCAGAGCAACCGAGAAAACGTGAAAAACGGTGTT
CAAACAAAAACTTTAATACGGACCCATTTTAA

Upstream 100 bases:

>100_bases
CAGACTTCTATTTCGGCGGCGAATATCGCACCGGCTGGCGGGCAGGGTTTGATTACCTGATGAAGCCAGAAACACTGCGT
AAAACCAGGGAAGGAGCATT

Downstream 100 bases:

>100_bases
CCACGCCTGACCAGCGTGAAATAACCGAGGAAAGACCAATGACCCAATTAATTAACCACACGAGTGCACACTATAGTGAC
GACGGTAAAAACTACCTGGA

Product: DnaB family helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MNPEELEAIVLAGLINGGATPDAFDVIATTPEEAFSIVFHRRAFSEIKKQALANGMIDMLFISEALGGSSLADLSQISRI
PATVPNLKGYAGKMVRAWRSRKMAALLQSGADGIRNAVNQSQRDEVIESHVAQVLDMSAATGTVQPVHIKELLPLYIDTV
DKRMSGDAQEMMLKTGVEDLDVALGGINMTDLVVVAGRPGMGKTEFTLKIIDGVTADGGGALFFSMEMGAQQIVERTVAG
AGNMSSSRLRNPKEMDDEDWSRLTAALAIMQDRDIWIVDATDLTIEQIRAIAETHKRRYPHLKLIGVDYMGLIKKPKAER
HDLAVGHISRNMKTMAMRLHTPVFALSQLSRQVDSRPAGQRRPVMSDLRDSGSVEQDADSILFLYRDDVYTPDSPAKGIA
EVIIGKNRSGGAGEIIYQEFRNGHFLPVDQHVAREKIRIQKEAEQPRKREKRCSNKNFNTDPF

Sequences:

>Translated_463_residues
MNPEELEAIVLAGLINGGATPDAFDVIATTPEEAFSIVFHRRAFSEIKKQALANGMIDMLFISEALGGSSLADLSQISRI
PATVPNLKGYAGKMVRAWRSRKMAALLQSGADGIRNAVNQSQRDEVIESHVAQVLDMSAATGTVQPVHIKELLPLYIDTV
DKRMSGDAQEMMLKTGVEDLDVALGGINMTDLVVVAGRPGMGKTEFTLKIIDGVTADGGGALFFSMEMGAQQIVERTVAG
AGNMSSSRLRNPKEMDDEDWSRLTAALAIMQDRDIWIVDATDLTIEQIRAIAETHKRRYPHLKLIGVDYMGLIKKPKAER
HDLAVGHISRNMKTMAMRLHTPVFALSQLSRQVDSRPAGQRRPVMSDLRDSGSVEQDADSILFLYRDDVYTPDSPAKGIA
EVIIGKNRSGGAGEIIYQEFRNGHFLPVDQHVAREKIRIQKEAEQPRKREKRCSNKNFNTDPF
>Mature_463_residues
MNPEELEAIVLAGLINGGATPDAFDVIATTPEEAFSIVFHRRAFSEIKKQALANGMIDMLFISEALGGSSLADLSQISRI
PATVPNLKGYAGKMVRAWRSRKMAALLQSGADGIRNAVNQSQRDEVIESHVAQVLDMSAATGTVQPVHIKELLPLYIDTV
DKRMSGDAQEMMLKTGVEDLDVALGGINMTDLVVVAGRPGMGKTEFTLKIIDGVTADGGGALFFSMEMGAQQIVERTVAG
AGNMSSSRLRNPKEMDDEDWSRLTAALAIMQDRDIWIVDATDLTIEQIRAIAETHKRRYPHLKLIGVDYMGLIKKPKAER
HDLAVGHISRNMKTMAMRLHTPVFALSQLSRQVDSRPAGQRRPVMSDLRDSGSVEQDADSILFLYRDDVYTPDSPAKGIA
EVIIGKNRSGGAGEIIYQEFRNGHFLPVDQHVAREKIRIQKEAEQPRKREKRCSNKNFNTDPF

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=426, Percent_Identity=32.1596244131455, Blast_Score=203, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51010; Mature: 51010

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPEELEAIVLAGLINGGATPDAFDVIATTPEEAFSIVFHRRAFSEIKKQALANGMIDML
CCHHHHHHHHHHHHHCCCCCCCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
FISEALGGSSLADLSQISRIPATVPNLKGYAGKMVRAWRSRKMAALLQSGADGIRNAVNQ
HHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCH
SQRDEVIESHVAQVLDMSAATGTVQPVHIKELLPLYIDTVDKRMSGDAQEMMLKTGVEDL
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHH
DVALGGINMTDLVVVAGRPGMGKTEFTLKIIDGVTADGGGALFFSMEMGAQQIVERTVAG
HHHHCCCCHHHEEEEECCCCCCCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHC
AGNMSSSRLRNPKEMDDEDWSRLTAALAIMQDRDIWIVDATDLTIEQIRAIAETHKRRYP
CCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
HLKLIGVDYMGLIKKPKAERHDLAVGHISRNMKTMAMRLHTPVFALSQLSRQVDSRPAGQ
CEEEECCCHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RRPVMSDLRDSGSVEQDADSILFLYRDDVYTPDSPAKGIAEVIIGKNRSGGAGEIIYQEF
CCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHH
RNGHFLPVDQHVAREKIRIQKEAEQPRKREKRCSNKNFNTDPF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNPEELEAIVLAGLINGGATPDAFDVIATTPEEAFSIVFHRRAFSEIKKQALANGMIDML
CCHHHHHHHHHHHHHCCCCCCCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
FISEALGGSSLADLSQISRIPATVPNLKGYAGKMVRAWRSRKMAALLQSGADGIRNAVNQ
HHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCH
SQRDEVIESHVAQVLDMSAATGTVQPVHIKELLPLYIDTVDKRMSGDAQEMMLKTGVEDL
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHH
DVALGGINMTDLVVVAGRPGMGKTEFTLKIIDGVTADGGGALFFSMEMGAQQIVERTVAG
HHHHCCCCHHHEEEEECCCCCCCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHC
AGNMSSSRLRNPKEMDDEDWSRLTAALAIMQDRDIWIVDATDLTIEQIRAIAETHKRRYP
CCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
HLKLIGVDYMGLIKKPKAERHDLAVGHISRNMKTMAMRLHTPVFALSQLSRQVDSRPAGQ
CEEEECCCHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RRPVMSDLRDSGSVEQDADSILFLYRDDVYTPDSPAKGIAEVIIGKNRSGGAGEIIYQEF
CCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHH
RNGHFLPVDQHVAREKIRIQKEAEQPRKREKRCSNKNFNTDPF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]