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The map label for this gene is gatY

Identifier: 157156604

GI number: 157156604

Start: 2357852

End: 2358706

Strand: Reverse

Name: gatY

Synonym: EcE24377A_2384

Alternate gene names: 157156604

Gene position: 2358706-2357852 (Counterclockwise)

Preceding gene: 157157113

Following gene: 157156384

Centisome position: 47.37

GC content: 53.1

Gene sequence:

>855_bases
ATGTACGTGGTATCGACAAAGCAGATGCTGAACAACGCACAGCGCGGCGGTTATGCGGTTCCGGCATTCAATATTCACAA
TCTCGAAACGATGCAAGTGGTGGTGGAAACCGCTGCCAACCTGCATGCGCCGGTCATCATCGCCGGAACGCCTGGCACAT
TTACTCATGCTGGTACAGAAAATCTGTTGGCGCTGGTCAGCGCGATGGCGAAGCAATATCACCATCCACTGACAATTCAT
CTCGACCATCACACGAAATTTGACGATATCGCTCAGAAGGTTCGTTCTGGCGTGCGCTCAGTCATGATTGACGCCTCGCA
TTTGCCTTTTGCGCAAAATATTTCACGGGTCAAAGAGGTGGTGGATTTTTGCCATCGCTTTGATGTCAGCGTCGAGGCGG
AGCTGGGGCAACTTGGCGGCCAGGAAGATGATGTGCAAGTCAATGAAGCCGATGCGTTGTACACCAACCCCGCACAGGCG
CGTGAATTTGCCGAGGCAACCGGGATTGATTCCCTGGCGGTCGCCATCGGTACGGCTCACGGTATGTATGCCAGCGCACC
GGTGCTTGATTTTTCTCGACTGGAGAACATTCGCCAGTGGGTGAACTTACCGCTGGTGCTGCATGGCGCGTCAGGATTAT
CGACTAAGGATATTCAGCAAACCATCAAAGTGGGGATATGCAAAATCAACGTTGCAACGGAGCTGAAAAATGCCTTCTCG
CAGGCGTTAAAAAATTACCTGACCGAGCACCCCGAAGCGACCGATCCCCGGGATTATTTGCAGTCGGCTAAATCCGCAAT
GCGCGATGTGGTGAGCAAAGTGATTGCCGATTGTGGCTGCGAGGGCAGGGCATAA

Upstream 100 bases:

>100_bases
ACAATTTTCATATTGTCGTTTTCGTGATCGTTATCTCGATATTTAAAAACAAATAATTTCATTATATTTTGAAATCGAAA
ACAAACGACAGGATATGAAA

Downstream 100 bases:

>100_bases
CGCACCTGCCATTTAACAAGGAAAAAACATGAAAACGTTAATTGCCCGGCATAAAGCTGGTGAACATATCGGCATATGTT
CAGTCTGTTCTGCCCATCCG

Product: tagatose-bisphosphate aldolase

Products: NA

Alternate protein names: TBPA; TagBP aldolase; D-tagatose-bisphosphate aldolase class II; Tagatose-bisphosphate aldolase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLTIH
LDHHTKFDDIAQKVRSGVRSVMIDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDIQQTIKVGICKINVATELKNAFS
QALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADCGCEGRA

Sequences:

>Translated_284_residues
MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLTIH
LDHHTKFDDIAQKVRSGVRSVMIDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDIQQTIKVGICKINVATELKNAFS
QALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADCGCEGRA
>Mature_284_residues
MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLTIH
LDHHTKFDDIAQKVRSGVRSVMIDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDIQQTIKVGICKINVATELKNAFS
QALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADCGCEGRA

Specific function: Catalytic subunit of the tagatose-1,6-bisphosphate aldolase GatYZ, which catalyzes the reversible aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to produce tagatose 1,6- bisphosphate (T

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family. TagBP aldolase GatY subfamily

Homologues:

Organism=Escherichia coli, GI87082041, Length=284, Percent_Identity=98.943661971831, Blast_Score=586, Evalue=1e-169,
Organism=Escherichia coli, GI1789526, Length=283, Percent_Identity=54.0636042402827, Blast_Score=330, Evalue=6e-92,
Organism=Escherichia coli, GI1788072, Length=234, Percent_Identity=41.8803418803419, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1789293, Length=324, Percent_Identity=25, Blast_Score=89, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6322790, Length=329, Percent_Identity=24.0121580547112, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATY_ECO24 (A7ZNR5)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001463444.1
- ProteinModelPortal:   A7ZNR5
- SMR:   A7ZNR5
- STRING:   A7ZNR5
- EnsemblBacteria:   EBESCT00000022454
- GeneID:   5587419
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_2384
- NMPDR:   fig|331111.3.peg.126
- eggNOG:   COG0191
- GeneTree:   EBGT00050000009021
- HOGENOM:   HBG327581
- OMA:   KMYVVST
- ProtClustDB:   PRK09195
- BioCyc:   ECOL331111:ECE24377A_2384-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_01294
- InterPro:   IPR013785
- InterPro:   IPR000771
- InterPro:   IPR011288
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF001359
- TIGRFAMs:   TIGR00167
- TIGRFAMs:   TIGR01858

Pfam domain/function: PF01116 F_bP_aldolase

EC number: =4.1.2.40

Molecular weight: Translated: 30856; Mature: 30856

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00602 ALDOLASE_CLASS_II_1; PS00806 ALDOLASE_CLASS_II_2

Important sites: ACT_SITE 82-82 BINDING 181-181

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVIIAGTPGTFTHAGTE
CEEECHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHH
NLLALVSAMAKQYHHPLTIHLDHHTKFDDIAQKVRSGVRSVMIDASHLPFAQNISRVKEV
HHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQAREFAEATGIDSLAVAIGTAH
HHHHHHCCCCEEHHHHHCCCCCCCCEECCCCCEECCHHHHHHHHHHHCCHHHHHHHHHHC
GMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDIQQTIKVGICKINVATELKNAFS
CCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHEEEHHHHHHHHHH
QALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADCGCEGRA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVIIAGTPGTFTHAGTE
CEEECHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHH
NLLALVSAMAKQYHHPLTIHLDHHTKFDDIAQKVRSGVRSVMIDASHLPFAQNISRVKEV
HHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQAREFAEATGIDSLAVAIGTAH
HHHHHHCCCCEEHHHHHCCCCCCCCEECCCCCEECCHHHHHHHHHHHCCHHHHHHHHHHC
GMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDIQQTIKVGICKINVATELKNAFS
CCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHEEEHHHHHHHHHH
QALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADCGCEGRA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA