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The map label for this gene is wcaE [H]

Identifier: 157156247

GI number: 157156247

Start: 2311915

End: 2312670

Strand: Reverse

Name: wcaE [H]

Synonym: EcE24377A_2348

Alternate gene names: 157156247

Gene position: 2312670-2311915 (Counterclockwise)

Preceding gene: 157157053

Following gene: 157155740

Centisome position: 46.44

GC content: 46.3

Gene sequence:

>756_bases
ATGTTGCTTAGCATAATCACTGTCGCGTTTCGTAACCTCGAAGGGATAGTCAAAACACATGCCTCGCTGGCGCATCTGGC
GCATCTGGCGCAGGCGGAAGATATCAGCTTCGAATGGATTGTTGTCGATGGCGGTTCCAACGACGGCACCCGTGAGTATC
TGGAAAATCTCAATGGTATCTTTAACCTACGCTTTGTCAGCGAGCCAGATAACGGTATCTACGACGCCATGAACAAAGGC
ATTGCGATGGCACAAGGCAAGTTCGCGTTGTTTCTCAACTCGGGCGATATTTTTCATCAGGATGCCGCAAATTTTGTCCG
TAAGTTAAAAATGCAAAAAGATAACGTGATGATCACCGGCGATGCGCTGCTGGATTTTGGCGACGGGCATAAAATTAAGC
GTAGTGCTAAACCGGGTTGGTATATTTATCACAGCCTGCCCGCCAGCCATCAGGCGATATTTTTCCCGGTATCCGGCTTG
AAAAAATGGCGTTATGACCTGGAATATAAAGTATCTTCCGATTACGCGCTGGCGGCTAAAATGTATAAAGCCGGTTATGC
ATTTAAAAAACTCAATGGCCTGGTGTCTGAATTTTCCATGGGTGGGGTATCAACCACCAATAATATGGAATTGTGTGCTG
ACGCCAAAAAAGTCCAACGGCAAATATTACATGTGCCTGGCTTCTGGGCAGAATTATCCTGGCATTTACGCCAACGTACT
ACCTCAAAGACGAAAGCCTTATATAACAAAAGCTGA

Upstream 100 bases:

>100_bases
TTTTTTACCGGTTCGATATTTAGCCCGGAATATGCATTTTTAATCGTCTGTCCGTTTATTTTGCGAAAAGCGTTAAATAT
TACGAGGTAAGAATAAGAAC

Downstream 100 bases:

>100_bases
ATATAAGGGAAAACGATGCAAGATTTAAGCGGTTTCTCGGTGCCGAAAGGGTTCCGGGGCGGCAACGCTATTAAAGTGCA
ATTATGGTGGGCAGTACAGG

Product: putative glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLLSIITVAFRNLEGIVKTHASLAHLAHLAQAEDISFEWIVVDGGSNDGTREYLENLNGIFNLRFVSEPDNGIYDAMNKG
IAMAQGKFALFLNSGDIFHQDAANFVRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGL
KKWRYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQILHVPGFWAELSWHLRQRT
TSKTKALYNKS

Sequences:

>Translated_251_residues
MLLSIITVAFRNLEGIVKTHASLAHLAHLAQAEDISFEWIVVDGGSNDGTREYLENLNGIFNLRFVSEPDNGIYDAMNKG
IAMAQGKFALFLNSGDIFHQDAANFVRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGL
KKWRYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQILHVPGFWAELSWHLRQRT
TSKTKALYNKS
>Mature_251_residues
MLLSIITVAFRNLEGIVKTHASLAHLAHLAQAEDISFEWIVVDGGSNDGTREYLENLNGIFNLRFVSEPDNGIYDAMNKG
IAMAQGKFALFLNSGDIFHQDAANFVRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGL
KKWRYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQILHVPGFWAELSWHLRQRT
TSKTKALYNKS

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788368, Length=250, Percent_Identity=98, Blast_Score=505, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 28177; Mature: 28177

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSIITVAFRNLEGIVKTHASLAHLAHLAQAEDISFEWIVVDGGSNDGTREYLENLNGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCE
FNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANFVRKLKMQKDNVMITG
EEEEEECCCCCCHHHHHCCCEEEECCCEEEEEECCCEEHHHHHHHHHHHHCCCCCEEEEE
DALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKWRYDLEYKVSSDYALAAK
CEEEECCCCCEEECCCCCCEEEEECCCCCCCEEEEECCCHHHHCCCEEEEECCCHHHHHH
MYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQILHVPGFWAELSWHLRQRT
HHHHCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TSKTKALYNKS
HHHHHHHCCCC
>Mature Secondary Structure
MLLSIITVAFRNLEGIVKTHASLAHLAHLAQAEDISFEWIVVDGGSNDGTREYLENLNGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCE
FNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANFVRKLKMQKDNVMITG
EEEEEECCCCCCHHHHHCCCEEEECCCEEEEEECCCEEHHHHHHHHHHHHCCCCCEEEEE
DALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKWRYDLEYKVSSDYALAAK
CEEEECCCCCEEECCCCCCEEEEECCCCCCCEEEEECCCHHHHCCCEEEEECCCHHHHHH
MYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQILHVPGFWAELSWHLRQRT
HHHHCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TSKTKALYNKS
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9278503 [H]