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The map label for this gene is mleN [H]

Identifier: 156980641

GI number: 156980641

Start: 2451298

End: 2452677

Strand: Reverse

Name: mleN [H]

Synonym: SAHV_2310

Alternate gene names: 156980641

Gene position: 2452677-2451298 (Counterclockwise)

Preceding gene: 156980642

Following gene: 156980640

Centisome position: 85.16

GC content: 35.07

Gene sequence:

>1380_bases
ATGAAAACCTCTTTCTTCATCGTTTGTGAGAAAGAGGTATTTTTAATTGGAAAGCAGGTAAAAAGGATGGAAGTACATAA
AAAGAGCAATGCTTGGGCATTATTCCCCTTGTTATTATTTGTGGCGTTGTTTTTAGGCGTAGGTATTATCACAGGTGATT
TTACTTCAATGCCATTAAATGTTGCAATTACGATAACGGTAATTGTGGCGTTATTAATGAATCGAAAAGAATCATTTGCT
AAAAAAGTTGAGGTCTTCACGAAAGGTGCAGGCCATTCAAACATTATTTTAATGATGTTGATTTTTATTTTAGCAGGTGC
ATTTTCAAATACAGCTGAAAAAATGGGTGGCGTAAAGTCGACAGTTAATTTAGGACTATCATTAATCCCAGAAAATTTAA
TTATTGTAGGATTGTTTGTGATTTGTATGTTTGTTTCTATATCTATGGGGACGTCTGTAGGAACTGTTGCTGCAATAGCA
CCTGTTGGTTACGGATTTGCACAAGCGACAGATGTACCAACTGCGCTAGCAATGGCTACAGTTGTTGGAGGTGCAATGTT
CGGCGATAATCTATCAATGATTTCAGATACAACAATTGCTGCGGTTAGAACACAACATACGAAAATGAAAGATAAATTCA
GAGTAAACTTTAAAATTGTATTGCCAGGTGCTATTTTAACGATTATTATTCTGTATTTCTTAACGAATGGCATTTCTTTA
AATCATGCTAAAAACTATGATTATAATCTAATTAAAGTTGTACCGTATATTTTAGTATTGGTATTAGCATTATTAGGTGT
AAATGTCATTATCGTATTGATTGGCGGAACATTATTAGCTGGAATCATTGGTCTCATAGATGGTTCATTCGGTTGGATGG
GACTACTAGATGCAGTGTCCAAAGGTATTATAAGCATGGAAGATATTGCTATGATTGCGTTGCTAATTGGTGGTTTAGTC
GGTATTATTCAACACAATGGTGGTATTGAATGGTTGTTGCAATTTGTTCGCTCTAAAGTGAAATCAAAGCGTGGCGCAGA
ATTAGGAATTGCGAGTTTGGTAAGTGTCGCAGATATTGCCACGGCAAACAACACCATCTCTATTATTATGTCAGGTCCTT
TAGCTAAAAATATTGCAGATGAATATGATGTAGATTCAAGGAAATCAGCAAGTATTCTTGATATATTTGGAGGCTGCTTC
CAAGGTTTATTACCATATAGCCCGCAAGTCATTTCAGCGGCTGGTGTAGCTGGTATTTCACCATTAATGTTATTCCCATA
CAGCATTTATCCTATATTATTAGGTGTATGCGGACTTATTGCGATTTTATTTAATTTTCCAAAGCTAAAAAAGAACACTA
GTCAAGAAATGAAACATTAA

Upstream 100 bases:

>100_bases
TCTAGAGAGGTGGAGGGACTGGCCCTATGAAGCCTCGGCAACATCTCGAATGTGCCAATTCCAGTAACCGTAATGGTTTG
AAGATAAGCAGGTAAAGCAC

Downstream 100 bases:

>100_bases
TGCTTAACAATATATCGATGTAGCCAAGTAAGGTGGCTTGTTATATTAACAGATCATGTTGTACTCACCGTATAACGCTA
ATCTTTTAAAAATGATGATC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MKTSFFIVCEKEVFLIGKQVKRMEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITITVIVALLMNRKESFA
KKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKSTVNLGLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIA
PVGYGFAQATDVPTALAMATVVGGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDAVSKGIISMEDIAMIALLIGGLV
GIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSVADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCF
QGLLPYSPQVISAAGVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH

Sequences:

>Translated_459_residues
MKTSFFIVCEKEVFLIGKQVKRMEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITITVIVALLMNRKESFA
KKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKSTVNLGLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIA
PVGYGFAQATDVPTALAMATVVGGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDAVSKGIISMEDIAMIALLIGGLV
GIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSVADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCF
QGLLPYSPQVISAAGVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH
>Mature_459_residues
MKTSFFIVCEKEVFLIGKQVKRMEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITITVIVALLMNRKESFA
KKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKSTVNLGLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIA
PVGYGFAQATDVPTALAMATVVGGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDAVSKGIISMEDIAMIALLIGGLV
GIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSVADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCF
QGLLPYSPQVISAAGVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 48952; Mature: 48952

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSFFIVCEKEVFLIGKQVKRMEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLN
CCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
VAITITVIVALLMNRKESFAKKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKS
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCHH
TVNLGLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMAT
HHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHCCCHHHHHHHH
VVGGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
HHHHHHCCCCCHHHCCHHHHHHHHHHHHHCCCEEEEEEEEECHHHHHHHHHHHHHCCCEE
NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDAVS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSVADIA
HCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAAGVAGIS
CCCCEEEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCC
PLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHCCC
>Mature Secondary Structure
MKTSFFIVCEKEVFLIGKQVKRMEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLN
CCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
VAITITVIVALLMNRKESFAKKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKS
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCHH
TVNLGLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMAT
HHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHCCCHHHHHHHH
VVGGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
HHHHHHCCCCCHHHCCHHHHHHHHHHHHHCCCEEEEEEEEECHHHHHHHHHHHHHCCCEE
NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDAVS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSVADIA
HCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAAGVAGIS
CCCCEEEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCC
PLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]