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The map label for this gene is trmI [H]

Identifier: 156937919

GI number: 156937919

Start: 1010635

End: 1011390

Strand: Direct

Name: trmI [H]

Synonym: Igni_1131

Alternate gene names: 156937919

Gene position: 1010635-1011390 (Clockwise)

Preceding gene: 156937918

Following gene: 156937920

Centisome position: 77.89

GC content: 58.33

Gene sequence:

>756_bases
GTGGAAGTAAGGGAAGGCGACCTGGTTCTGATATACATAGACAAGAGGAGGAAGAAGTTAGTGAAAGCGATTAAAGGAAA
GGTATTTGGGAGTGACTTAGGAGTGTTGAAGCTTGACGACGTAATTGGTAAGAGGTACGGCGAGAGCGTGGAGTTCAGCA
CGGGGGCTAGGGCTTATATCCTCAAGCCCACGCTGGAGGACGTACTCTTCTCATTTAAGAGAAGAACGCAAGTGATCTAC
CCGAAGGACTTGGGGTTGATGGCGATCAAGCTGGGGGCAGGCCCGGGGAAGAGGTGCTTGGAGGGCGGCTCCGGGTCGGG
CTTCGTGGCAGCTACCTTGAGCTGGTTCGGCTGCGACGTGGCCTCCTTCGAGGTGAGGAAGGAACACTTGTACGTTGCCA
AAAGGAACTTAGAGGAGGTCGGATTGAGGGTAACCTTCGTGAACGCTTCCATAGGGGAGGCCCCGGAGGTCTACGGCGAG
GAGAGCTTCGACGCGGCGGCTGTGGACGTGGGAGACCCTTGGAAGTACGTGGACGGGGTGTGGAGGGCCCTCAAGGGCGG
CGCCCCGGCGGCCTTCTGGCTGCCCACCTACAACCAGCTGGAGAAGCTGAAGGAGGCCTCGCGGGGCAAGTTCATTTGGA
TGGAGAGCCTCGAGGTCAACGAGAGGAGGCTCAAGGTCGAGAAGGGCGCCACCAGGCCCGAGCAGACGGGGATAACCTTC
ACGGGCTTCTGGGCCTTCCTTAGGAAGGTTTACTGA

Upstream 100 bases:

>100_bases
GAAATTAACAAATATAAAAGTTTGGTAATGAGTTATACAGAAGCATCAATGGGCGTCCCGTGCCCTATATTTCCGCCCGG
GACCGCGGGCTGGGGAGAGA

Downstream 100 bases:

>100_bases
TTCCCAGTTAAAAGCTTTCATCAGAAAGCCCAAGTTCTATTATATGAGATTTCTCCTCAAAGCTTTCACGTGGGACGTCA
GAAAAGGTTCCCCTTCCTCA

Product: tRNA methyltransferase complex GCD14 subunit

Products: NA

Alternate protein names: tRNA(m1A58)-methyltransferase; tRNA(m1A58)MTase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MEVREGDLVLIYIDKRRKKLVKAIKGKVFGSDLGVLKLDDVIGKRYGESVEFSTGARAYILKPTLEDVLFSFKRRTQVIY
PKDLGLMAIKLGAGPGKRCLEGGSGSGFVAATLSWFGCDVASFEVRKEHLYVAKRNLEEVGLRVTFVNASIGEAPEVYGE
ESFDAAAVDVGDPWKYVDGVWRALKGGAPAAFWLPTYNQLEKLKEASRGKFIWMESLEVNERRLKVEKGATRPEQTGITF
TGFWAFLRKVY

Sequences:

>Translated_251_residues
MEVREGDLVLIYIDKRRKKLVKAIKGKVFGSDLGVLKLDDVIGKRYGESVEFSTGARAYILKPTLEDVLFSFKRRTQVIY
PKDLGLMAIKLGAGPGKRCLEGGSGSGFVAATLSWFGCDVASFEVRKEHLYVAKRNLEEVGLRVTFVNASIGEAPEVYGE
ESFDAAAVDVGDPWKYVDGVWRALKGGAPAAFWLPTYNQLEKLKEASRGKFIWMESLEVNERRLKVEKGATRPEQTGITF
TGFWAFLRKVY
>Mature_251_residues
MEVREGDLVLIYIDKRRKKLVKAIKGKVFGSDLGVLKLDDVIGKRYGESVEFSTGARAYILKPTLEDVLFSFKRRTQVIY
PKDLGLMAIKLGAGPGKRCLEGGSGSGFVAATLSWFGCDVASFEVRKEHLYVAKRNLEEVGLRVTFVNASIGEAPEVYGE
ESFDAAAVDVGDPWKYVDGVWRALKGGAPAAFWLPTYNQLEKLKEASRGKFIWMESLEVNERRLKVEKGATRPEQTGITF
TGFWAFLRKVY

Specific function: Catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in tRNA. Is required for cell growth at extreme temperatures [H]

COG id: COG2519

COG function: function code J; tRNA(1-methyladenosine) methyltransferase and related methyltransferases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmI family [H]

Homologues:

Organism=Homo sapiens, GI222831587, Length=234, Percent_Identity=29.0598290598291, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI123173772, Length=246, Percent_Identity=27.6422764227642, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17509459, Length=238, Percent_Identity=26.0504201680672, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322336, Length=145, Percent_Identity=30.3448275862069, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24650257, Length=255, Percent_Identity=27.843137254902, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014816 [H]

Pfam domain/function: PF08704 GCD14 [H]

EC number: =2.1.1.36 [H]

Molecular weight: Translated: 28031; Mature: 28031

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVREGDLVLIYIDKRRKKLVKAIKGKVFGSDLGVLKLDDVIGKRYGESVEFSTGARAYI
CCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHCCCCEEECCCCEEEE
LKPTLEDVLFSFKRRTQVIYPKDLGLMAIKLGAGPGKRCLEGGSGSGFVAATLSWFGCDV
ECCCHHHHHHHHHHCCEEECCCCCCEEEEEECCCCCHHHHCCCCCCCEEEEEHHHHCCCH
ASFEVRKEHLYVAKRNLEEVGLRVTFVNASIGEAPEVYGEESFDAAAVDVGDPWKYVDGV
HHHHHHHHHHHHHHCCHHHCCEEEEEEECCCCCCCHHCCCCCCCEEEEECCCCHHHHHHH
WRALKGGAPAAFWLPTYNQLEKLKEASRGKFIWMESLEVNERRLKVEKGATRPEQTGITF
HHHHCCCCCEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCHHEEECCCCCCCHHCCCEE
TGFWAFLRKVY
HHHHHHHHHCC
>Mature Secondary Structure
MEVREGDLVLIYIDKRRKKLVKAIKGKVFGSDLGVLKLDDVIGKRYGESVEFSTGARAYI
CCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHCCCCEEECCCCEEEE
LKPTLEDVLFSFKRRTQVIYPKDLGLMAIKLGAGPGKRCLEGGSGSGFVAATLSWFGCDV
ECCCHHHHHHHHHHCCEEECCCCCCEEEEEECCCCCHHHHCCCCCCCEEEEEHHHHCCCH
ASFEVRKEHLYVAKRNLEEVGLRVTFVNASIGEAPEVYGEESFDAAAVDVGDPWKYVDGV
HHHHHHHHHHHHHHCCHHHCCEEEEEEECCCCCCCHHCCCCCCCEEEEECCCCHHHHHHH
WRALKGGAPAAFWLPTYNQLEKLKEASRGKFIWMESLEVNERRLKVEKGATRPEQTGITF
HHHHCCCCCEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCHHEEECCCCCCCHHCCCEE
TGFWAFLRKVY
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA