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The map label for this gene is nuoBCD [H]

Identifier: 156937335

GI number: 156937335

Start: 486259

End: 487881

Strand: Reverse

Name: nuoBCD [H]

Synonym: Igni_0542

Alternate gene names: 156937335

Gene position: 487881-486259 (Counterclockwise)

Preceding gene: 156937336

Following gene: 156937333

Centisome position: 37.6

GC content: 57.49

Gene sequence:

>1623_bases
ATGAACTTCGTGGGTGAGGTCGAGAGGAAGGAGTGGAGCAACGCCCTATACGTCGAGGTAGAGCCCCAGTACTGGTACGA
GAACGCGGCGAGGCTGAAGGACCAAGGCTACTTGTTCCCCTCTATGTTAACGGCCGTGGACTGGCCCGCCGAGAAGAAGG
TGCAAGTGTTCTTCTTAGTTCACAGAATAAGGGACGCCAGCTCGGTAGTCCTCAGCACAAAGGTGGACAGGGAGAACAGG
GAGCTGCCCAGCCTGAGCTCCTTGTACCCTGGCGTGGAGTGGCACGAGAACGAGACGTACGAGATGTTCGGGATAAACTT
CACCGGCCACCCCGACTACGAGAGGTGGGGAAGGATAAGGAAGCTGTTGCTCAGCGAGGACCCGAGGCTCTTCCCGAAGG
ACCGCTTCCCGTTGCTGAAGGACTCCGGGGGCTACAAGGACTTGCCGAAGGTCAACTGGGCCGAGATAGAGGGCACTGAC
CAAGAGAGGGAGGGGCTGAGGGAGGACCTAACGTACGTCTACGTGGGCCCCCAGCACCCGGCCACCCACGGGCCCGTGGG
CTTCTTGTTAGGCTTGAAGGGAGAGATAGTAGAGGACGTCATACCTCGCTTGGGCTACGTCCACAGGGGCGTGGAGTGGA
TCTACGAGCAGAAGGAGTACTTAAAGGTAATACCGCTGTTGGACAGGCAATGCTACATAGACGGCATAGGCTGGGAGTGG
CCCTACGTAATGGCCTTGGAAGAGATAATGGACGTTAAGGTCGATAGGAGGGCACAGCTGTTGAGGATTCTGGCGGCCGA
GCTGAGCAGGATACAGTCCCACTTGCTCTACATAGGTTCCTTCGCCGAGAACGTGAACCACCTAACTGCTTTCGCTTGGA
CCGTTAGGGACAGGGAAGTCTTCATCAACTTGCTAGAAATGTTGACTGGCCAGAGGATGACCTTCAACTACTTGAGGCCC
GGCGGGGTCGCCTTTGACCTCCCCCAAGGGTTTAAGGACGTGTACGAGAAGGTCGTTGCCAGGTTCCACAAGCATTTCGA
GAAGTTAATTGAAACAACCTTAGACAACCCCACGGTAGCCATGAGGGCCGAGGGGGTAGCGCAAGTGAGCGCCGAGGACG
CCATAAAGTACGGCTGGAGCGGGCCTAACCTAAGGGCCTCCGGGGTAGCTTGGGACTTGAGGATAAACAAGCCCTACGGA
GGCTACGACGACGGGATAGACTTCGAGGGGGCGGTCGGGAGCGTAGGGGACAACTTGGACAGAATGCTGGTCAGGTTCGA
GGAGATACGCCAGAGCTTGAAGATAATTAGGCAAGTCCTAAGGGAGCTGACTGAGAGGGGGCAGAACGAGCTCGGCACGC
ACTTGAAGAGGGTCCCGCCGTTCCCGCGCAAGCCCGGAATAGCTTACGGCTACCACGACAGCCCCAGGGGAGAGAACGGG
ATCTTGTTGGTGGCCAACCCCTCCGACCCCAAGTGGAGGACCAGGCCGTGGAGGGTACACATGAGGTCCCCGGTCTTCGT
ACACACGATGAGCTTGAAGTACCTAGGGAAGGGACTTCTCTTACAAGACTTCCTAGCGTTCTACATATCTCTCGACACGT
GTGTCGCGGAGATGGACCGCTAG

Upstream 100 bases:

>100_bases
TCACGCGTTATCGCCTACACGACGAGGAGGGCTCGGACAAGAAGGTCGTGGACCCCATGCTCCGTTATGCCTACAACAAC
AGAAAGGCGGGTGAGAAGAG

Downstream 100 bases:

>100_bases
AGGCCGCTCTCAATCAACGCTTTTATCGCTTCTTTTTCTTCTTTCACTTCCTTCAACTTCGCCTCGAGCTTCTGCACCTC
CTCCGAGCTCGGCAACAAGT

Product: NADH-ubiquinone oxidoreductase, chain 49kDa

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B/C/D; NDH-1 subunit B/C/D [H]

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MNFVGEVERKEWSNALYVEVEPQYWYENAARLKDQGYLFPSMLTAVDWPAEKKVQVFFLVHRIRDASSVVLSTKVDRENR
ELPSLSSLYPGVEWHENETYEMFGINFTGHPDYERWGRIRKLLLSEDPRLFPKDRFPLLKDSGGYKDLPKVNWAEIEGTD
QEREGLREDLTYVYVGPQHPATHGPVGFLLGLKGEIVEDVIPRLGYVHRGVEWIYEQKEYLKVIPLLDRQCYIDGIGWEW
PYVMALEEIMDVKVDRRAQLLRILAAELSRIQSHLLYIGSFAENVNHLTAFAWTVRDREVFINLLEMLTGQRMTFNYLRP
GGVAFDLPQGFKDVYEKVVARFHKHFEKLIETTLDNPTVAMRAEGVAQVSAEDAIKYGWSGPNLRASGVAWDLRINKPYG
GYDDGIDFEGAVGSVGDNLDRMLVRFEEIRQSLKIIRQVLRELTERGQNELGTHLKRVPPFPRKPGIAYGYHDSPRGENG
ILLVANPSDPKWRTRPWRVHMRSPVFVHTMSLKYLGKGLLLQDFLAFYISLDTCVAEMDR

Sequences:

>Translated_540_residues
MNFVGEVERKEWSNALYVEVEPQYWYENAARLKDQGYLFPSMLTAVDWPAEKKVQVFFLVHRIRDASSVVLSTKVDRENR
ELPSLSSLYPGVEWHENETYEMFGINFTGHPDYERWGRIRKLLLSEDPRLFPKDRFPLLKDSGGYKDLPKVNWAEIEGTD
QEREGLREDLTYVYVGPQHPATHGPVGFLLGLKGEIVEDVIPRLGYVHRGVEWIYEQKEYLKVIPLLDRQCYIDGIGWEW
PYVMALEEIMDVKVDRRAQLLRILAAELSRIQSHLLYIGSFAENVNHLTAFAWTVRDREVFINLLEMLTGQRMTFNYLRP
GGVAFDLPQGFKDVYEKVVARFHKHFEKLIETTLDNPTVAMRAEGVAQVSAEDAIKYGWSGPNLRASGVAWDLRINKPYG
GYDDGIDFEGAVGSVGDNLDRMLVRFEEIRQSLKIIRQVLRELTERGQNELGTHLKRVPPFPRKPGIAYGYHDSPRGENG
ILLVANPSDPKWRTRPWRVHMRSPVFVHTMSLKYLGKGLLLQDFLAFYISLDTCVAEMDR
>Mature_540_residues
MNFVGEVERKEWSNALYVEVEPQYWYENAARLKDQGYLFPSMLTAVDWPAEKKVQVFFLVHRIRDASSVVLSTKVDRENR
ELPSLSSLYPGVEWHENETYEMFGINFTGHPDYERWGRIRKLLLSEDPRLFPKDRFPLLKDSGGYKDLPKVNWAEIEGTD
QEREGLREDLTYVYVGPQHPATHGPVGFLLGLKGEIVEDVIPRLGYVHRGVEWIYEQKEYLKVIPLLDRQCYIDGIGWEW
PYVMALEEIMDVKVDRRAQLLRILAAELSRIQSHLLYIGSFAENVNHLTAFAWTVRDREVFINLLEMLTGQRMTFNYLRP
GGVAFDLPQGFKDVYEKVVARFHKHFEKLIETTLDNPTVAMRAEGVAQVSAEDAIKYGWSGPNLRASGVAWDLRINKPYG
GYDDGIDFEGAVGSVGDNLDRMLVRFEEIRQSLKIIRQVLRELTERGQNELGTHLKRVPPFPRKPGIAYGYHDSPRGENG
ILLVANPSDPKWRTRPWRVHMRSPVFVHTMSLKYLGKGLLLQDFLAFYISLDTCVAEMDR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0649

COG function: function code C; NADH:ubiquinone oxidoreductase 49 kD subunit 7

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=449, Percent_Identity=32.0712694877506, Blast_Score=230, Evalue=3e-60,
Organism=Homo sapiens, GI260898743, Length=436, Percent_Identity=32.1100917431193, Blast_Score=223, Evalue=3e-58,
Organism=Escherichia coli, GI145693162, Length=522, Percent_Identity=29.8850574712644, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1789076, Length=479, Percent_Identity=28.6012526096033, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI1788832, Length=481, Percent_Identity=28.8981288981289, Blast_Score=155, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17555284, Length=438, Percent_Identity=32.648401826484, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17568379, Length=438, Percent_Identity=31.9634703196347, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24638644, Length=388, Percent_Identity=34.7938144329897, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI221459469, Length=388, Percent_Identity=34.5360824742268, Blast_Score=241, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010219
- InterPro:   IPR010218
- InterPro:   IPR001135
- InterPro:   IPR006137
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR020396
- InterPro:   IPR006138
- InterPro:   IPR022885
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 62428; Mature: 62428

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFVGEVERKEWSNALYVEVEPQYWYENAARLKDQGYLFPSMLTAVDWPAEKKVQVFFLV
CCCCCCCHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHH
HRIRDASSVVLSTKVDRENRELPSLSSLYPGVEWHENETYEMFGINFTGHPDYERWGRIR
HHHCCCHHEEEEEECCCCCCCCCCHHHHCCCCEECCCCCEEEEEEECCCCCCHHHHHHHH
KLLLSEDPRLFPKDRFPLLKDSGGYKDLPKVNWAEIEGTDQEREGLREDLTYVYVGPQHP
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHCCEEEEECCCCC
ATHGPVGFLLGLKGEIVEDVIPRLGYVHRGVEWIYEQKEYLKVIPLLDRQCYIDGIGWEW
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
PYVMALEEIMDVKVDRRAQLLRILAAELSRIQSHLLYIGSFAENVNHLTAFAWTVRDREV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHH
FINLLEMLTGQRMTFNYLRPGGVAFDLPQGFKDVYEKVVARFHKHFEKLIETTLDNPTVA
HHHHHHHHCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
MRAEGVAQVSAEDAIKYGWSGPNLRASGVAWDLRINKPYGGYDDGIDFEGAVGSVGDNLD
EECCCHHHCCHHHHHHCCCCCCCCEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHH
RMLVRFEEIRQSLKIIRQVLRELTERGQNELGTHLKRVPPFPRKPGIAYGYHDSPRGENG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCC
ILLVANPSDPKWRTRPWRVHMRSPVFVHTMSLKYLGKGLLLQDFLAFYISLDTCVAEMDR
EEEEECCCCCCCCCCCEEEEECCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFVGEVERKEWSNALYVEVEPQYWYENAARLKDQGYLFPSMLTAVDWPAEKKVQVFFLV
CCCCCCCHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHH
HRIRDASSVVLSTKVDRENRELPSLSSLYPGVEWHENETYEMFGINFTGHPDYERWGRIR
HHHCCCHHEEEEEECCCCCCCCCCHHHHCCCCEECCCCCEEEEEEECCCCCCHHHHHHHH
KLLLSEDPRLFPKDRFPLLKDSGGYKDLPKVNWAEIEGTDQEREGLREDLTYVYVGPQHP
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHCCEEEEECCCCC
ATHGPVGFLLGLKGEIVEDVIPRLGYVHRGVEWIYEQKEYLKVIPLLDRQCYIDGIGWEW
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
PYVMALEEIMDVKVDRRAQLLRILAAELSRIQSHLLYIGSFAENVNHLTAFAWTVRDREV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHH
FINLLEMLTGQRMTFNYLRPGGVAFDLPQGFKDVYEKVVARFHKHFEKLIETTLDNPTVA
HHHHHHHHCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
MRAEGVAQVSAEDAIKYGWSGPNLRASGVAWDLRINKPYGGYDDGIDFEGAVGSVGDNLD
EECCCHHHCCHHHHHHCCCCCCCCEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHH
RMLVRFEEIRQSLKIIRQVLRELTERGQNELGTHLKRVPPFPRKPGIAYGYHDSPRGENG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCC
ILLVANPSDPKWRTRPWRVHMRSPVFVHTMSLKYLGKGLLLQDFLAFYISLDTCVAEMDR
EEEEECCCCCCCCCCCEEEEECCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA