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The map label for this gene is hmeA [H]

Identifier: 156937322

GI number: 156937322

Start: 471937

End: 472998

Strand: Reverse

Name: hmeA [H]

Synonym: Igni_0529

Alternate gene names: 156937322

Gene position: 472998-471937 (Counterclockwise)

Preceding gene: 156937323

Following gene: 156937321

Centisome position: 36.45

GC content: 57.25

Gene sequence:

>1062_bases
ATGACCGTCAGCAGAGACGAAGTAGGTAAGGTCCTACACAAGCTCAGCGACGAAGAGCTGCTGGGCTACGTGTACGAGGA
GGGGGAGTACGTAATAAGCGAGTACAGCCCCTATAAGACCCCAGAAGAGATCAAAGAGCTAGCGAAGAAGAAGGAGGAGA
TGGCTAAGAAGAAGTGCATGGAGAGCGCCAACTCCATATGCCAGAGGCTGGGCGAGAACAGCCCGGAGTGTAGGCTGGCG
AGGAAGCACTGCGAGGAGATCAAGGTAAAGTGGACCTTGGCTAAGAAGGGAGTCCGCTGGGCCATGGCACTGGACTTGAA
CAAGTGTATAGGCTGTAGGAGGTGCGCCTACGCTTGTGTACAAGAGAACAACGTGGACCGCACCCAAGGCATAGAGTGGA
TAAAGGTAGTCAACGTGAACAGGGAGGAGCTCGAGCTGCTCGGAGTAGACCTAGACTACAAGGAGGCCCCTTACAAGGAT
AGGGTCTACATACCGGTCGCGTGCAACCAGTGCGAGTACCCGCCGTGCACTATGGTTTGTCCGGTTAGGGCCACTTGGCA
AGAGGCCGACGGGATAGTCGTCGTGGACAGCTACCGGTGCATAGGCTGTAGGTACTGCATAACCGCTTGTCCTTACGGAG
CTAGGCACATGAACTGGAAGCCAGTCACCGTGGACGCGCTGACGCTCAACCCCAACATGCACGTGTTCGGGAACGTCCCC
AGGGAGGTGCATACCGTAGAGAAGTGTACTTGGTGTATACAGAGGACCAGGGACGGGGGGACCACGGCTTGTGTGGAGAT
ATGTCCGGTGGGCTCGAGGGCCTTCGGCAACCTCCACGACGAGGAAGGGCCCATACAGAGAATAATAAAGGAGTACGGGG
TGTTCGTCCTCAAGCCCTACGCCGGCACTAAGCCGAGGTTCTTCTACTACTTCGGCCCCGCTAGGACCCCTCCGCTGGAC
TCCGAGAACCCCAAGTCCGGCAACTGGCTGGGCACCCCGAAGCACGGCGAAGGGACTCACCACGGAAGTAGAGAAAAAGC
AGAGGAGAGGGGTCACGACTGA

Upstream 100 bases:

>100_bases
ATGTTAAGAAGTAACGCGAAGTGGGGGTGGTGGGACCATGGCTAACCAGACCCGTAGGAATTTGCTCAAGGGGATAGCCG
CGGCCGGCGCGGTAGGCTTG

Downstream 100 bases:

>100_bases
GGAGCGACGGAGGAGGTGAGGGGCAGTGAGCAACCCAGTGGTAGAGTTTCTTGTGACCATATGGTACATAATAAAGCACA
TGTTCAAGGGCGACTGGAGG

Product: sulfide reductase, subunit B

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MTVSRDEVGKVLHKLSDEELLGYVYEEGEYVISEYSPYKTPEEIKELAKKKEEMAKKKCMESANSICQRLGENSPECRLA
RKHCEEIKVKWTLAKKGVRWAMALDLNKCIGCRRCAYACVQENNVDRTQGIEWIKVVNVNREELELLGVDLDYKEAPYKD
RVYIPVACNQCEYPPCTMVCPVRATWQEADGIVVVDSYRCIGCRYCITACPYGARHMNWKPVTVDALTLNPNMHVFGNVP
REVHTVEKCTWCIQRTRDGGTTACVEICPVGSRAFGNLHDEEGPIQRIIKEYGVFVLKPYAGTKPRFFYYFGPARTPPLD
SENPKSGNWLGTPKHGEGTHHGSREKAEERGHD

Sequences:

>Translated_353_residues
MTVSRDEVGKVLHKLSDEELLGYVYEEGEYVISEYSPYKTPEEIKELAKKKEEMAKKKCMESANSICQRLGENSPECRLA
RKHCEEIKVKWTLAKKGVRWAMALDLNKCIGCRRCAYACVQENNVDRTQGIEWIKVVNVNREELELLGVDLDYKEAPYKD
RVYIPVACNQCEYPPCTMVCPVRATWQEADGIVVVDSYRCIGCRYCITACPYGARHMNWKPVTVDALTLNPNMHVFGNVP
REVHTVEKCTWCIQRTRDGGTTACVEICPVGSRAFGNLHDEEGPIQRIIKEYGVFVLKPYAGTKPRFFYYFGPARTPPLD
SENPKSGNWLGTPKHGEGTHHGSREKAEERGHD
>Mature_352_residues
TVSRDEVGKVLHKLSDEELLGYVYEEGEYVISEYSPYKTPEEIKELAKKKEEMAKKKCMESANSICQRLGENSPECRLAR
KHCEEIKVKWTLAKKGVRWAMALDLNKCIGCRRCAYACVQENNVDRTQGIEWIKVVNVNREELELLGVDLDYKEAPYKDR
VYIPVACNQCEYPPCTMVCPVRATWQEADGIVVVDSYRCIGCRYCITACPYGARHMNWKPVTVDALTLNPNMHVFGNVPR
EVHTVEKCTWCIQRTRDGGTTACVEICPVGSRAFGNLHDEEGPIQRIIKEYGVFVLKPYAGTKPRFFYYFGPARTPPLDS
ENPKSGNWLGTPKHGEGTHHGSREKAEERGHD

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=222, Percent_Identity=31.981981981982, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI226510944, Length=223, Percent_Identity=31.8385650224215, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787122, Length=220, Percent_Identity=29.0909090909091, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787872, Length=220, Percent_Identity=29.5454545454545, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790326, Length=180, Percent_Identity=32.7777777777778, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787749, Length=184, Percent_Identity=29.8913043478261, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789370, Length=191, Percent_Identity=31.9371727748691, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87082180, Length=187, Percent_Identity=29.9465240641711, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1787478, Length=127, Percent_Identity=33.0708661417323, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082179, Length=174, Percent_Identity=31.6091954022989, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787740, Length=118, Percent_Identity=33.0508474576271, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787963, Length=112, Percent_Identity=33.9285714285714, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789067, Length=175, Percent_Identity=34.2857142857143, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367245, Length=111, Percent_Identity=36.9369369369369, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 40180; Mature: 40049

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
5.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSRDEVGKVLHKLSDEELLGYVYEEGEYVISEYSPYKTPEEIKELAKKKEEMAKKKCM
CCCCHHHHHHHHHHCCCHHHHEEEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
ESANSICQRLGENSPECRLARKHCEEIKVKWTLAKKGVRWAMALDLNKCIGCRRCAYACV
HHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEEECCCCEEEEEECHHHHHHHHHHHHHHH
QENNVDRTQGIEWIKVVNVNREELELLGVDLDYKEAPYKDRVYIPVACNQCEYPPCTMVC
HCCCCCHHHCCCEEEEECCCHHHEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCEEEE
PVRATWQEADGIVVVDSYRCIGCRYCITACPYGARHMNWKPVTVDALTLNPNMHVFGNVP
CCCCCHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCEEEECCCC
REVHTVEKCTWCIQRTRDGGTTACVEICPVGSRAFGNLHDEEGPIQRIIKEYGVFVLKPY
HHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHCCCCCCCCCHHHHHHHHCCEEEEECC
AGTKPRFFYYFGPARTPPLDSENPKSGNWLGTPKHGEGTHHGSREKAEERGHD
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
TVSRDEVGKVLHKLSDEELLGYVYEEGEYVISEYSPYKTPEEIKELAKKKEEMAKKKCM
CCCHHHHHHHHHHCCCHHHHEEEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
ESANSICQRLGENSPECRLARKHCEEIKVKWTLAKKGVRWAMALDLNKCIGCRRCAYACV
HHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEEECCCCEEEEEECHHHHHHHHHHHHHHH
QENNVDRTQGIEWIKVVNVNREELELLGVDLDYKEAPYKDRVYIPVACNQCEYPPCTMVC
HCCCCCHHHCCCEEEEECCCHHHEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCEEEE
PVRATWQEADGIVVVDSYRCIGCRYCITACPYGARHMNWKPVTVDALTLNPNMHVFGNVP
CCCCCHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCEEEECCCC
REVHTVEKCTWCIQRTRDGGTTACVEICPVGSRAFGNLHDEEGPIQRIIKEYGVFVLKPY
HHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHCCCCCCCCCHHHHHHHHCCEEEEECC
AGTKPRFFYYFGPARTPPLDSENPKSGNWLGTPKHGEGTHHGSREKAEERGHD
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]