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The map label for this gene is dkgB [H]

Identifier: 156937242

GI number: 156937242

Start: 397166

End: 397906

Strand: Reverse

Name: dkgB [H]

Synonym: Igni_0448

Alternate gene names: 156937242

Gene position: 397906-397166 (Counterclockwise)

Preceding gene: 156937243

Following gene: 156937240

Centisome position: 30.67

GC content: 59.38

Gene sequence:

>741_bases
GTGAGAGAGGCGGCAGAGTTGCGCAGGATAGGGGGCGAGGCGGTCTCGGACATAGGTCTGGGGTCTTGGGCGGTTAGGGA
CCCCAAGTCCTTCGTAGAAACCTTGGTTAAGGCATTCGAAATGGGAATAAACTTGGTGGACACGGCGGAGATGTATAGGA
CCGAGCCCCTCGTGAGGGAAGCTATGGAAATTTACGGTAGCAAGGAAATATTCGTGACCACCAAGCTCTACCCGTGGAAC
GTGAGGCACGACTGCGAGAGGGCCCTCAGGAGGCAAGTGGAGAGGCTCGGGAAGGTGCCCGACCTAACCTTGGTCCACTG
GCCGGAGGACATAGCCACCGAGGTGAGGGAGCTGGAGAAGATGGTTGACAAGGGGCTCACGAGGATGATAGGCGTCTCCA
ACGTTACGAAGGAACAGCTGGAGGTGGCCCTCCACGCGACCAAGAAGCACGAAATAGTTGCGGTCCAGAACAAGTACAGC
CCCACCTTCACCGCCACGGAGGAGCTCTTCCCCACGTTGGAGGAGAAGGGCATAGCGTTGCAAGCCCACACCCCTTTGGA
CAGGGGGAGGGCGCTGGAGCTGGACTGGGTGAGGAGGAAGGCGGAGGAGCTGGGCGCGCCCCCGGCGGCAATAGTAATAG
CTTGGCTCCTCTCGCGCTCCCCGGTGGTCATACCGATCCCTAAGACGGAAAGGGTGGAGCACTTGATGGAGCTCTTGGAG
GGGACGAAGATAAAGCTCTAG

Upstream 100 bases:

>100_bases
ACTGGGTCGTTTACGTAAAGAGCTTGCCCTTGAGGGGTTCCCAGTTCGCCGGCGTGAGTGTCCATAGGCCCTCTCAAGTG
TTTAAGGTCAAGCCGTACAA

Downstream 100 bases:

>100_bases
CCTAACGCTTGGACCTCCATCCTCCCCGTCAGCCCGAAGCCCTCCCACGAGACCATGAAGGCCTCCCCCTCTATGCCGTC
GTGGTAAAGCAAGTAGAACA

Product: aldo/keto reductase

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MREAAELRRIGGEAVSDIGLGSWAVRDPKSFVETLVKAFEMGINLVDTAEMYRTEPLVREAMEIYGSKEIFVTTKLYPWN
VRHDCERALRRQVERLGKVPDLTLVHWPEDIATEVRELEKMVDKGLTRMIGVSNVTKEQLEVALHATKKHEIVAVQNKYS
PTFTATEELFPTLEEKGIALQAHTPLDRGRALELDWVRRKAEELGAPPAAIVIAWLLSRSPVVIPIPKTERVEHLMELLE
GTKIKL

Sequences:

>Translated_246_residues
MREAAELRRIGGEAVSDIGLGSWAVRDPKSFVETLVKAFEMGINLVDTAEMYRTEPLVREAMEIYGSKEIFVTTKLYPWN
VRHDCERALRRQVERLGKVPDLTLVHWPEDIATEVRELEKMVDKGLTRMIGVSNVTKEQLEVALHATKKHEIVAVQNKYS
PTFTATEELFPTLEEKGIALQAHTPLDRGRALELDWVRRKAEELGAPPAAIVIAWLLSRSPVVIPIPKTERVEHLMELLE
GTKIKL
>Mature_246_residues
MREAAELRRIGGEAVSDIGLGSWAVRDPKSFVETLVKAFEMGINLVDTAEMYRTEPLVREAMEIYGSKEIFVTTKLYPWN
VRHDCERALRRQVERLGKVPDLTLVHWPEDIATEVRELEKMVDKGLTRMIGVSNVTKEQLEVALHATKKHEIVAVQNKYS
PTFTATEELFPTLEEKGIALQAHTPLDRGRALELDWVRRKAEELGAPPAAIVIAWLLSRSPVVIPIPKTERVEHLMELLE
GTKIKL

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=257, Percent_Identity=29.5719844357977, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI5174391, Length=257, Percent_Identity=29.5719844357977, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI310109922, Length=226, Percent_Identity=30.9734513274336, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787674, Length=269, Percent_Identity=29.368029739777, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1786400, Length=221, Percent_Identity=29.4117647058824, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1788081, Length=242, Percent_Identity=29.3388429752066, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082198, Length=233, Percent_Identity=30.0429184549356, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788070, Length=218, Percent_Identity=27.5229357798165, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17561300, Length=231, Percent_Identity=27.7056277056277, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6324694, Length=250, Percent_Identity=28.4, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322556, Length=244, Percent_Identity=29.0983606557377, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24644950, Length=277, Percent_Identity=26.7148014440433, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 27911; Mature: 27911

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREAAELRRIGGEAVSDIGLGSWAVRDPKSFVETLVKAFEMGINLVDTAEMYRTEPLVRE
CCCHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHH
AMEIYGSKEIFVTTKLYPWNVRHDCERALRRQVERLGKVPDLTLVHWPEDIATEVRELEK
HHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHH
MVDKGLTRMIGVSNVTKEQLEVALHATKKHEIVAVQNKYSPTFTATEELFPTLEEKGIAL
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCEEE
QAHTPLDRGRALELDWVRRKAEELGAPPAAIVIAWLLSRSPVVIPIPKTERVEHLMELLE
EECCCCCCCCEECHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHC
GTKIKL
CCCCCC
>Mature Secondary Structure
MREAAELRRIGGEAVSDIGLGSWAVRDPKSFVETLVKAFEMGINLVDTAEMYRTEPLVRE
CCCHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHH
AMEIYGSKEIFVTTKLYPWNVRHDCERALRRQVERLGKVPDLTLVHWPEDIATEVRELEK
HHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHH
MVDKGLTRMIGVSNVTKEQLEVALHATKKHEIVAVQNKYSPTFTATEELFPTLEEKGIAL
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCEEE
QAHTPLDRGRALELDWVRRKAEELGAPPAAIVIAWLLSRSPVVIPIPKTERVEHLMELLE
EECCCCCCCCEECHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHC
GTKIKL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]