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The map label for this gene is purB [H]

Identifier: 156937194

GI number: 156937194

Start: 353476

End: 354813

Strand: Reverse

Name: purB [H]

Synonym: Igni_0400

Alternate gene names: 156937194

Gene position: 354813-353476 (Counterclockwise)

Preceding gene: 156937199

Following gene: 156937193

Centisome position: 27.35

GC content: 58.22

Gene sequence:

>1338_bases
TTGCACGTCTGCCCGTTCCAGTGGAGGTACGGCAGCGAAGAGATGAGGAAGATAATGTCCCAAGAGGAGCGCTTGAAGAA
GATGGCGTTGGTAGAGTATGCGTTGCTGAAGGCGTTGAAGGAGGTCGGGGAGGTCCCGGAGGAGGTGGACCCGGAGGAGG
TCTTGAGGGCCGCGGAGGAGGTGACGGTCGAGGAGGTAGAGGAGCTGGAGAGGGAGTTAGGCCACGACGTAATGGCCTTC
TTAGAGGCCCTCAGGAGGAGGACCAAGACCGCTGGTAAGTACTTGCACTTGGGGGCCACGAGCTACGACGTGGTGGACAC
CGCTTGGGCCTTGATAATAAGGGACGCCCTAGCTAAGGTGTATAAAGACTTAAAGGAGATAATCAAGAAGTTCCTCGAAA
TAAGCGAGGAGTACGCGGACTTGCCCATGGTAGGTAGGACCCACGGCCAGTGGGCCGTCCCCATAACCTTGGGCTTCAAG
TTCGCAAACTACGCCTACGAGCTGGCTAGGAGCTACGAGAGGCTGAAGGAGGCCGAGAGGAGGGTGGTGAGGCTGAAGAT
GAGCGGCGCCGTGGGGACCATGGCCGCTTGGGGCGAGAAGGCCTTCGAGGTGGAGAGGCTCGTCTCCGAGGAGCTCAAGC
TCCCTCCGCACCCCATAACGACCCAAGTGGCCCCCAGGGACGGCTTCGCCGAGCTCTTGGCGGACTTGGCTATACTCTCG
TCCGTACTGGACAGGTTTGCCTTGGAGGTGAGGGAGCTCAGCAGGCCGGAGATAAGGGAGCTGGTGGAGGGGGTGAGGAG
GGGACAAGTTGGCTCCAGCACCATGCCCCACAAGGCCAACCCCGTGACCTCCGAGAAGATATCTGGGCTCGCGAAGGTGA
TGAGGTCCTTGGTCTTGGGGGAGCTCGAGAACGTGCCCTTGTGGCACGAGAGGGACTTAACGAACTCGGCCTCCGAAAGG
GTGCTCATCCCCCACGCCTTCTTGACCGTGGACGAACAGCTCAAGTCTACCAAATCTCTATTAAACAAGCTTATAATTAA
CGAAGAGGCAATAAGGAAGCACTTAGAGGAGGCCGGCTGCGAGGTGTTGGCAGAAAGGGTAATGATCTACCTAACGAAAG
AGAAGGGGCTCGCGAGGAACGACGCCCACAAGGTAGTGATGGACGCCATTAGGGTTCACGGGAGCTTAAGGAGGGCCTTG
GAGGAGGGGCACGAGCTGTCCAAGTACTTGACCTCTGAGGAGCTCGAGGCGCTGTGCGACCCCAGCAGTTACTTGGGAAA
GGCTAGAGAGTTGATAGCTAGGACAGCGAAGTACGTCGAGGAGGTAGTAGGCCTTTGA

Upstream 100 bases:

>100_bases
CGTTCCCGAAAGTGGTCAAGATGACTATCAACGCCCCTTCCCCTAGACCCTCGCCCTTCGCCTTCATTACTCCATCGCCG
CCCCCTCCCCGGCGGTCGCC

Downstream 100 bases:

>100_bases
GGGCCGTGGTGCTCGCAGGGGGGAACAGGGTAAAGAGGTTCAAGACGTACTTCTTAGGGAAGATGGTCGTCGAATACCCC
CTAAAAGCCTTGGAAAAGGT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MHVCPFQWRYGSEEMRKIMSQEERLKKMALVEYALLKALKEVGEVPEEVDPEEVLRAAEEVTVEEVEELERELGHDVMAF
LEALRRRTKTAGKYLHLGATSYDVVDTAWALIIRDALAKVYKDLKEIIKKFLEISEEYADLPMVGRTHGQWAVPITLGFK
FANYAYELARSYERLKEAERRVVRLKMSGAVGTMAAWGEKAFEVERLVSEELKLPPHPITTQVAPRDGFAELLADLAILS
SVLDRFALEVRELSRPEIRELVEGVRRGQVGSSTMPHKANPVTSEKISGLAKVMRSLVLGELENVPLWHERDLTNSASER
VLIPHAFLTVDEQLKSTKSLLNKLIINEEAIRKHLEEAGCEVLAERVMIYLTKEKGLARNDAHKVVMDAIRVHGSLRRAL
EEGHELSKYLTSEELEALCDPSSYLGKARELIARTAKYVEEVVGL

Sequences:

>Translated_445_residues
MHVCPFQWRYGSEEMRKIMSQEERLKKMALVEYALLKALKEVGEVPEEVDPEEVLRAAEEVTVEEVEELERELGHDVMAF
LEALRRRTKTAGKYLHLGATSYDVVDTAWALIIRDALAKVYKDLKEIIKKFLEISEEYADLPMVGRTHGQWAVPITLGFK
FANYAYELARSYERLKEAERRVVRLKMSGAVGTMAAWGEKAFEVERLVSEELKLPPHPITTQVAPRDGFAELLADLAILS
SVLDRFALEVRELSRPEIRELVEGVRRGQVGSSTMPHKANPVTSEKISGLAKVMRSLVLGELENVPLWHERDLTNSASER
VLIPHAFLTVDEQLKSTKSLLNKLIINEEAIRKHLEEAGCEVLAERVMIYLTKEKGLARNDAHKVVMDAIRVHGSLRRAL
EEGHELSKYLTSEELEALCDPSSYLGKARELIARTAKYVEEVVGL
>Mature_445_residues
MHVCPFQWRYGSEEMRKIMSQEERLKKMALVEYALLKALKEVGEVPEEVDPEEVLRAAEEVTVEEVEELERELGHDVMAF
LEALRRRTKTAGKYLHLGATSYDVVDTAWALIIRDALAKVYKDLKEIIKKFLEISEEYADLPMVGRTHGQWAVPITLGFK
FANYAYELARSYERLKEAERRVVRLKMSGAVGTMAAWGEKAFEVERLVSEELKLPPHPITTQVAPRDGFAELLADLAILS
SVLDRFALEVRELSRPEIRELVEGVRRGQVGSSTMPHKANPVTSEKISGLAKVMRSLVLGELENVPLWHERDLTNSASER
VLIPHAFLTVDEQLKSTKSLLNKLIINEEAIRKHLEEAGCEVLAERVMIYLTKEKGLARNDAHKVVMDAIRVHGSLRRAL
EEGHELSKYLTSEELEALCDPSSYLGKARELIARTAKYVEEVVGL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=456, Percent_Identity=26.7543859649123, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=27.0341207349081, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI19743875, Length=307, Percent_Identity=28.6644951140065, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1787376, Length=392, Percent_Identity=29.0816326530612, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790398, Length=289, Percent_Identity=29.0657439446367, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17508577, Length=329, Percent_Identity=30.0911854103343, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32564234, Length=297, Percent_Identity=29.6296296296296, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17553882, Length=349, Percent_Identity=25.7879656160458, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI32565146, Length=343, Percent_Identity=26.2390670553936, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323391, Length=467, Percent_Identity=27.6231263383298, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24647570, Length=451, Percent_Identity=25.2771618625277, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24640177, Length=347, Percent_Identity=27.3775216138329, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640179, Length=347, Percent_Identity=27.3775216138329, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24662684, Length=337, Percent_Identity=25.8160237388724, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI78710009, Length=235, Percent_Identity=30.2127659574468, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50438; Mature: 50438

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVCPFQWRYGSEEMRKIMSQEERLKKMALVEYALLKALKEVGEVPEEVDPEEVLRAAEE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
VTVEEVEELERELGHDVMAFLEALRRRTKTAGKYLHLGATSYDVVDTAWALIIRDALAKV
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHH
YKDLKEIIKKFLEISEEYADLPMVGRTHGQWAVPITLGFKFANYAYELARSYERLKEAER
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHEEHHHHHHHHHHHHHHHHHHHHHHH
RVVRLKMSGAVGTMAAWGEKAFEVERLVSEELKLPPHPITTQVAPRDGFAELLADLAILS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
SVLDRFALEVRELSRPEIRELVEGVRRGQVGSSTMPHKANPVTSEKISGLAKVMRSLVLG
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ELENVPLWHERDLTNSASERVLIPHAFLTVDEQLKSTKSLLNKLIINEEAIRKHLEEAGC
HHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
EVLAERVMIYLTKEKGLARNDAHKVVMDAIRVHGSLRRALEEGHELSKYLTSEELEALCD
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PSSYLGKARELIARTAKYVEEVVGL
CHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHVCPFQWRYGSEEMRKIMSQEERLKKMALVEYALLKALKEVGEVPEEVDPEEVLRAAEE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
VTVEEVEELERELGHDVMAFLEALRRRTKTAGKYLHLGATSYDVVDTAWALIIRDALAKV
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHH
YKDLKEIIKKFLEISEEYADLPMVGRTHGQWAVPITLGFKFANYAYELARSYERLKEAER
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHEEHHHHHHHHHHHHHHHHHHHHHHH
RVVRLKMSGAVGTMAAWGEKAFEVERLVSEELKLPPHPITTQVAPRDGFAELLADLAILS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
SVLDRFALEVRELSRPEIRELVEGVRRGQVGSSTMPHKANPVTSEKISGLAKVMRSLVLG
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ELENVPLWHERDLTNSASERVLIPHAFLTVDEQLKSTKSLLNKLIINEEAIRKHLEEAGC
HHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
EVLAERVMIYLTKEKGLARNDAHKVVMDAIRVHGSLRRALEEGHELSKYLTSEELEALCD
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PSSYLGKARELIARTAKYVEEVVGL
CHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]