Click here to switch to the map view.

The map label for this gene is dapE [H]

Identifier: 156937109

GI number: 156937109

Start: 271268

End: 272425

Strand: Reverse

Name: dapE [H]

Synonym: Igni_0315

Alternate gene names: 156937109

Gene position: 272425-271268 (Counterclockwise)

Preceding gene: 156937111

Following gene: 156937108

Centisome position: 21.0

GC content: 59.41

Gene sequence:

>1158_bases
TTGTCCCCCCAAGAGGTCTTGGACCTCCTCTCCCAACTCATCTCCTTCGACACCGTCTCCCCCGAAGGCAAGCAGTACGA
GGACTTGGTCCACTTCCTCAAGGGCTGGCTGGAGGAGAGGGGAGTCAGCGCTAAGGTTGAGTACGTGGACGACGAGTATA
GGAGCTCCCACTGCCCCCAAGGCCCCAAGCCCTTGCTCTTCGCTTGGGTGGGCGAGGGGGAGCCCTTACTCGAGTTCAAC
GGCCATTACGACGTGGTCCCTCCCGGCGACGGGTGGGAGGGGAACCCCTTCGAGCCCAAGGTGGTGGGCGAGTACTTGGT
GGGACGGGGGGCCACGGACATGAAGGGGGGTGTGGCGGCAGTTGCTGCTTCCCTCGCGGAGCTCTCGAACTGGAAGGGTA
ACAAGGTGCAAGCGGTCTTCGTGCCGGACGAGGAAGTTGGGGGGAGGTGCGGGACCGGGTACAGGGTGAGCAAACTGAAG
GAGAAGTACGAGATCCCGAGGAGGGTCGTAGTGGCCGAGCCCACGGACCACAACGTGTGGATAGGCCACAAGGGGGTGGT
TTGGCTGAGAGTGGAAGTGCCGGGGAAGCAAGTGCACGCTTCCACACCCTGGATGGGGGAAAATGCCTTTGTGAAGGCTT
CTGCTTTGGTATTGGAGCTCAATAGGGCCCTCACGGACAAGTTTTCCAAGAGGTACAGCAAGTACGAATACTCGCCGGAA
CATCCGCTGACGAAGTTTAACGCATTCAACATAGGTGGAGTGGCGTACTCCACATCAAACAAGGAGAACGTGGTGCCCGG
TTCTTTCGTTTTCAGCGTGGACGTGAGGGTGATCCCGGAGGAGGACGAGCGCGAGGTCGTGGAGTTCGTAAAGGAAGTAA
TAGGGGATAGGGCGAAGGTAGAGGTAAAGCTCCTAGAGCCTGGAATATTGAACGAGAACAGCGAGCTGGCCAAACTGATA
AACGAGGTGTGGGGCGAGCCGCTGAAGGTCCACAAGGCGGCCTCCGACATGAGGTACTATAGGGGATACGACGTCGTCAC
TTGGGGGCCCGGCGACCACAGAGAGTCCCACACGCCCAATGAGAAGATAAAGATAAGTGAGGTCGTGGAGTTCGTGGGGC
GTTACGCGCGGCTGGCGAAGCTCCTAGCCGGGGGCTAG

Upstream 100 bases:

>100_bases
GAACTAGGGGTTATGATGTGGACGCTCTCCCCTCGAACTCCTTACGGAGGCGGAAATAGCTTATCACCTCCGAGCCCTTA
CTAGAGACCCGGTGCCCTCT

Downstream 100 bases:

>100_bases
TAGCTCGAAATGCGACAATTATTACCGTCGGTTCGAGTCCCGCAGGGGTTCTAAGGTTTGTTACACTGGACCGGATACCC
TCCCTTAGTTTCGGCTGCGC

Product: acetylornithine deacetylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSPQEVLDLLSQLISFDTVSPEGKQYEDLVHFLKGWLEERGVSAKVEYVDDEYRSSHCPQGPKPLLFAWVGEGEPLLEFN
GHYDVVPPGDGWEGNPFEPKVVGEYLVGRGATDMKGGVAAVAASLAELSNWKGNKVQAVFVPDEEVGGRCGTGYRVSKLK
EKYEIPRRVVVAEPTDHNVWIGHKGVVWLRVEVPGKQVHASTPWMGENAFVKASALVLELNRALTDKFSKRYSKYEYSPE
HPLTKFNAFNIGGVAYSTSNKENVVPGSFVFSVDVRVIPEEDEREVVEFVKEVIGDRAKVEVKLLEPGILNENSELAKLI
NEVWGEPLKVHKAASDMRYYRGYDVVTWGPGDHRESHTPNEKIKISEVVEFVGRYARLAKLLAGG

Sequences:

>Translated_385_residues
MSPQEVLDLLSQLISFDTVSPEGKQYEDLVHFLKGWLEERGVSAKVEYVDDEYRSSHCPQGPKPLLFAWVGEGEPLLEFN
GHYDVVPPGDGWEGNPFEPKVVGEYLVGRGATDMKGGVAAVAASLAELSNWKGNKVQAVFVPDEEVGGRCGTGYRVSKLK
EKYEIPRRVVVAEPTDHNVWIGHKGVVWLRVEVPGKQVHASTPWMGENAFVKASALVLELNRALTDKFSKRYSKYEYSPE
HPLTKFNAFNIGGVAYSTSNKENVVPGSFVFSVDVRVIPEEDEREVVEFVKEVIGDRAKVEVKLLEPGILNENSELAKLI
NEVWGEPLKVHKAASDMRYYRGYDVVTWGPGDHRESHTPNEKIKISEVVEFVGRYARLAKLLAGG
>Mature_384_residues
SPQEVLDLLSQLISFDTVSPEGKQYEDLVHFLKGWLEERGVSAKVEYVDDEYRSSHCPQGPKPLLFAWVGEGEPLLEFNG
HYDVVPPGDGWEGNPFEPKVVGEYLVGRGATDMKGGVAAVAASLAELSNWKGNKVQAVFVPDEEVGGRCGTGYRVSKLKE
KYEIPRRVVVAEPTDHNVWIGHKGVVWLRVEVPGKQVHASTPWMGENAFVKASALVLELNRALTDKFSKRYSKYEYSPEH
PLTKFNAFNIGGVAYSTSNKENVVPGSFVFSVDVRVIPEEDEREVVEFVKEVIGDRAKVEVKLLEPGILNENSELAKLIN
EVWGEPLKVHKAASDMRYYRGYDVVTWGPGDHRESHTPNEKIKISEVVEFVGRYARLAKLLAGG

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=287, Percent_Identity=27.8745644599303, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790395, Length=351, Percent_Identity=21.9373219373219, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789236, Length=415, Percent_Identity=23.6144578313253, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 42926; Mature: 42795

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPQEVLDLLSQLISFDTVSPEGKQYEDLVHFLKGWLEERGVSAKVEYVDDEYRSSHCPQ
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCC
GPKPLLFAWVGEGEPLLEFNGHYDVVPPGDGWEGNPFEPKVVGEYLVGRGATDMKGGVAA
CCCCEEEEEECCCCEEEEECCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHH
VAASLAELSNWKGNKVQAVFVPDEEVGGRCGTGYRVSKLKEKYEIPRRVVVAEPTDHNVW
HHHHHHHHHCCCCCEEEEEECCCHHHCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCEE
IGHKGVVWLRVEVPGKQVHASTPWMGENAFVKASALVLELNRALTDKFSKRYSKYEYSPE
ECCCCEEEEEEECCCCEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
HPLTKFNAFNIGGVAYSTSNKENVVPGSFVFSVDVRVIPEEDEREVVEFVKEVIGDRAKV
CCCCEECCEECCCEEEECCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEE
EVKLLEPGILNENSELAKLINEVWGEPLKVHKAASDMRYYRGYDVVTWGPGDHRESHTPN
EEEEECCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
EKIKISEVVEFVGRYARLAKLLAGG
CCEEHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SPQEVLDLLSQLISFDTVSPEGKQYEDLVHFLKGWLEERGVSAKVEYVDDEYRSSHCPQ
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCC
GPKPLLFAWVGEGEPLLEFNGHYDVVPPGDGWEGNPFEPKVVGEYLVGRGATDMKGGVAA
CCCCEEEEEECCCCEEEEECCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHH
VAASLAELSNWKGNKVQAVFVPDEEVGGRCGTGYRVSKLKEKYEIPRRVVVAEPTDHNVW
HHHHHHHHHCCCCCEEEEEECCCHHHCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCEE
IGHKGVVWLRVEVPGKQVHASTPWMGENAFVKASALVLELNRALTDKFSKRYSKYEYSPE
ECCCCEEEEEEECCCCEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
HPLTKFNAFNIGGVAYSTSNKENVVPGSFVFSVDVRVIPEEDEREVVEFVKEVIGDRAKV
CCCCEECCEECCCEEEECCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEE
EVKLLEPGILNENSELAKLINEVWGEPLKVHKAASDMRYYRGYDVVTWGPGDHRESHTPN
EEEEECCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
EKIKISEVVEFVGRYARLAKLLAGG
CCEEHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA