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The map label for this gene is leuD1 [H]

Identifier: 156937098

GI number: 156937098

Start: 259042

End: 259602

Strand: Reverse

Name: leuD1 [H]

Synonym: Igni_0304

Alternate gene names: 156937098

Gene position: 259602-259042 (Counterclockwise)

Preceding gene: 156937099

Following gene: 156937097

Centisome position: 20.01

GC content: 55.97

Gene sequence:

>561_bases
GTGGCTAGTCAAAGGCTGAAAAGCCCTCCGACGCAAGGACGCGAGGGCAGCGACATGATCAAGGGTAGAGTGGTCGCTAA
GCTCGGCGACAACATAGACACGGACATGATAATACCCTTCAGGTACAAGGCGAAGACCACAGACTACTACGAGCTGGCGA
AGCACGTGTTCGAGGACTTGGACCCCGAGCTGGCGAAGAGGATCAAGCCCGGGGACGTGGTGGTGGCGGGCAAGAACTTC
GGGATGGGGAGCTCCAGGGAGCAAGCGCCAATAGCGATCAAGTACTCCGGGGTGAGCGCAGTAATAGCTAAGAGCTTCGC
CAGGATATTCTTTAGGAACGCAATAAACATAGGCCTCCCGGTCATAGAGGCCCCGGAGGTAGTGGATGAGACAGAGGAGG
GCGACGAGCTCGAGGTGGACTTGGAGAAGGGCGTAATACGGAACCTCACCAAGGGGAAGGAGTACTCGTTCAAACCCTAC
CCCCCTATGATAATGGAGGTAATGAAGGAAGGGGGGATATACGAGTACTTCAAGAAGCACGGGAAGTTCCCGTGGGAGTG
A

Upstream 100 bases:

>100_bases
GTATGGACACCGACAAGTACTTCGAACTGATGGAAGGTGGAGTAGATTCGAGGGAGCTAGCTGAGGTCAAGGCCTTCGAG
GACCCCGGGGAGGCGCTGGA

Downstream 100 bases:

>100_bases
ACCGTGAAAACCCTCCTTTTTGAGGGCCCCCGGGGAAGCGGTTGCCCCAGTACGCGGTAATAACCGTAGTCGGGAAGGAC
CGGGTAGGCATAGTAGCCGG

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MASQRLKSPPTQGREGSDMIKGRVVAKLGDNIDTDMIIPFRYKAKTTDYYELAKHVFEDLDPELAKRIKPGDVVVAGKNF
GMGSSREQAPIAIKYSGVSAVIAKSFARIFFRNAINIGLPVIEAPEVVDETEEGDELEVDLEKGVIRNLTKGKEYSFKPY
PPMIMEVMKEGGIYEYFKKHGKFPWE

Sequences:

>Translated_186_residues
MASQRLKSPPTQGREGSDMIKGRVVAKLGDNIDTDMIIPFRYKAKTTDYYELAKHVFEDLDPELAKRIKPGDVVVAGKNF
GMGSSREQAPIAIKYSGVSAVIAKSFARIFFRNAINIGLPVIEAPEVVDETEEGDELEVDLEKGVIRNLTKGKEYSFKPY
PPMIMEVMKEGGIYEYFKKHGKFPWE
>Mature_185_residues
ASQRLKSPPTQGREGSDMIKGRVVAKLGDNIDTDMIIPFRYKAKTTDYYELAKHVFEDLDPELAKRIKPGDVVVAGKNFG
MGSSREQAPIAIKYSGVSAVIAKSFARIFFRNAINIGLPVIEAPEVVDETEEGDELEVDLEKGVIRNLTKGKEYSFKPYP
PMIMEVMKEGGIYEYFKKHGKFPWE

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320440, Length=113, Percent_Identity=40.7079646017699, Blast_Score=85, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011824
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 20909; Mature: 20778

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASQRLKSPPTQGREGSDMIKGRVVAKLGDNIDTDMIIPFRYKAKTTDYYELAKHVFEDL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCC
DPELAKRIKPGDVVVAGKNFGMGSSREQAPIAIKYSGVSAVIAKSFARIFFRNAINIGLP
CHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCC
VIEAPEVVDETEEGDELEVDLEKGVIRNLTKGKEYSFKPYPPMIMEVMKEGGIYEYFKKH
CCCCCHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHC
GKFPWE
CCCCCC
>Mature Secondary Structure 
ASQRLKSPPTQGREGSDMIKGRVVAKLGDNIDTDMIIPFRYKAKTTDYYELAKHVFEDL
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCC
DPELAKRIKPGDVVVAGKNFGMGSSREQAPIAIKYSGVSAVIAKSFARIFFRNAINIGLP
CHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCC
VIEAPEVVDETEEGDELEVDLEKGVIRNLTKGKEYSFKPYPPMIMEVMKEGGIYEYFKKH
CCCCCHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHC
GKFPWE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]