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The map label for this gene is thrA [C]

Identifier: 156936994

GI number: 156936994

Start: 176932

End: 178317

Strand: Reverse

Name: thrA [C]

Synonym: Igni_0199

Alternate gene names: 156936994

Gene position: 178317-176932 (Counterclockwise)

Preceding gene: 156936995

Following gene: 156936992

Centisome position: 13.74

GC content: 56.13

Gene sequence:

>1386_bases
ATGAAGTTAGTCGCCAAGTTCGGCGGTTCCGTGCTAAGAGACGCGGAGGGCTACTCGGCGTCGGCCAAAGTGGTCAAGGA
GTGCGTCGGAGAAGGCTACAAGGTAGTTGTAGTGGTCTCGGCGATGAAGGGGGTTACCGACTCCTTGCTCAAGGTTTCCG
GGAGCCTACCCAAGGACTGGAAGAAGTACGTAGAAGCGCTCAAGAGGCGCCACGAAAGGGTCCTCCAACAGCTGGGCTCG
TCGGAGAGGTGGTTCAAGGAGTTAGAGAAGAACGTAGAAGAGCTCAAAAACGTGCTCTGGGCACTTTACATCCTCAGGGA
ACATACGCCCAGAACGAGAGACTTCGTCTTATCATTCGGCGAGAGGCTCTCCACGATACTCATGTCCGCCGCCCTGGAGA
GGGAGGGACTGAACCCCATCCCCCTGACGCCCGAAAAGGCTGGGATATTCACGGACGAGAACTACAACGAAGCGGTACCC
CTGTACGACGTCATAGAGAGGGAAGTTCCCAAGAGGTTGGAACCCTTCCTAGAGGAGGACAAAGTGCCCGTGGTCACCGG
CTTCTTGGGCTCGACGCTTGAAGGGAGCATAACTACCTTGGGGCGCGGGGGGAGCGACCTAACGGCTACTATACTGGGCT
CCTCCCTAAAGGCGGACGAGGTAAGGCTGTATACTGACATAGACGGCATAAAGACCGGGAACCCGAAGGAGTTCCCGGAC
GCCGTAACCATTAGGGAGCTCTCCGTAGAAGAGGCGATAGAGTTGGCCCACCTAGGGGCGAAGAGGTTGCACCCGCGTAC
GTTCGAACCTGTCCTCAGAAGGCACGTGGACGTGAGGGTGCTAAGCCTCTACGACCCCAAGGGCAACAACACCCTAATCT
CCCACGGGCGCAGGGGACCTATGTTGAAGGCGGTGGCATTGCTGAACGGGTTAGCGATAATAACTGTGAAGGGCGTTGGA
ATGGTCGGAACCAAGGGAGTCGCGGCCACGGTAATGGAATCGGTGACCAAAGTGGGAGCCAACATTTACGCGATCTCTCA
GCCGGTCTCCGAGATCTCCATATCTATTGCGGTTAAGTCCAGCAAGGCGGACCTAGTCTCCAACGCCATCAGGAACGCCC
TCGAGGGGAAGGGGATATCCGTAGAGACTAAGATAAAAGACAACGTGACTGCTGTGTCAGTTGTGGGCAGGGGGCTCGAG
GAGTTAGACTTCGTGGGCAACGTGCTCTCGCTCTTCGACAACAAGCCCGTTCTGATGGTCTCCAAAGGGCCACTCGACCA
GAGCTTAACGGTACTTACAACAAACAAGGCATCGAGGGAGATCGGGCGCAAAATACACGAGAAGATAGTTGAGGAGATGA
TGCAGTACTACGGCGAGGCCAAATAG

Upstream 100 bases:

>100_bases
CTTGATCGACACCTTACAAACCAACCTAAACGCCATGTTTGGAGGCGCATAAAGGAAAAGCTATTTTTTAAGACATCGGC
CCTCCGCCCGCGGGCTCCAG

Downstream 100 bases:

>100_bases
ATGTTGTCCTTGTTCTGTCTCACCTTTACCTTGATCCTCCTCCCCACGTCTACGTCAGCGTTGACGACAGTCATGACCAC
GTCCCTCCTCCCCACCGGCA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKLVAKFGGSVLRDAEGYSASAKVVKECVGEGYKVVVVVSAMKGVTDSLLKVSGSLPKDWKKYVEALKRRHERVLQQLGS
SERWFKELEKNVEELKNVLWALYILREHTPRTRDFVLSFGERLSTILMSAALEREGLNPIPLTPEKAGIFTDENYNEAVP
LYDVIEREVPKRLEPFLEEDKVPVVTGFLGSTLEGSITTLGRGGSDLTATILGSSLKADEVRLYTDIDGIKTGNPKEFPD
AVTIRELSVEEAIELAHLGAKRLHPRTFEPVLRRHVDVRVLSLYDPKGNNTLISHGRRGPMLKAVALLNGLAIITVKGVG
MVGTKGVAATVMESVTKVGANIYAISQPVSEISISIAVKSSKADLVSNAIRNALEGKGISVETKIKDNVTAVSVVGRGLE
ELDFVGNVLSLFDNKPVLMVSKGPLDQSLTVLTTNKASREIGRKIHEKIVEEMMQYYGEAK

Sequences:

>Translated_461_residues
MKLVAKFGGSVLRDAEGYSASAKVVKECVGEGYKVVVVVSAMKGVTDSLLKVSGSLPKDWKKYVEALKRRHERVLQQLGS
SERWFKELEKNVEELKNVLWALYILREHTPRTRDFVLSFGERLSTILMSAALEREGLNPIPLTPEKAGIFTDENYNEAVP
LYDVIEREVPKRLEPFLEEDKVPVVTGFLGSTLEGSITTLGRGGSDLTATILGSSLKADEVRLYTDIDGIKTGNPKEFPD
AVTIRELSVEEAIELAHLGAKRLHPRTFEPVLRRHVDVRVLSLYDPKGNNTLISHGRRGPMLKAVALLNGLAIITVKGVG
MVGTKGVAATVMESVTKVGANIYAISQPVSEISISIAVKSSKADLVSNAIRNALEGKGISVETKIKDNVTAVSVVGRGLE
ELDFVGNVLSLFDNKPVLMVSKGPLDQSLTVLTTNKASREIGRKIHEKIVEEMMQYYGEAK
>Mature_461_residues
MKLVAKFGGSVLRDAEGYSASAKVVKECVGEGYKVVVVVSAMKGVTDSLLKVSGSLPKDWKKYVEALKRRHERVLQQLGS
SERWFKELEKNVEELKNVLWALYILREHTPRTRDFVLSFGERLSTILMSAALEREGLNPIPLTPEKAGIFTDENYNEAVP
LYDVIEREVPKRLEPFLEEDKVPVVTGFLGSTLEGSITTLGRGGSDLTATILGSSLKADEVRLYTDIDGIKTGNPKEFPD
AVTIRELSVEEAIELAHLGAKRLHPRTFEPVLRRHVDVRVLSLYDPKGNNTLISHGRRGPMLKAVALLNGLAIITVKGVG
MVGTKGVAATVMESVTKVGANIYAISQPVSEISISIAVKSSKADLVSNAIRNALEGKGISVETKIKDNVTAVSVVGRGLE
ELDFVGNVLSLFDNKPVLMVSKGPLDQSLTVLTTNKASREIGRKIHEKIVEEMMQYYGEAK

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=467, Percent_Identity=26.5524625267666, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI1790455, Length=454, Percent_Identity=26.2114537444934, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790376, Length=297, Percent_Identity=28.956228956229, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320893, Length=467, Percent_Identity=25.0535331905782, Blast_Score=136, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 50449; Mature: 50449

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVAKFGGSVLRDAEGYSASAKVVKECVGEGYKVVVVVSAMKGVTDSLLKVSGSLPKDW
CCCHHHHCCHHHHCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHCCCCCHHH
KKYVEALKRRHERVLQQLGSSERWFKELEKNVEELKNVLWALYILREHTPRTRDFVLSFG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ERLSTILMSAALEREGLNPIPLTPEKAGIFTDENYNEAVPLYDVIEREVPKRLEPFLEED
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
KVPVVTGFLGSTLEGSITTLGRGGSDLTATILGSSLKADEVRLYTDIDGIKTGNPKEFPD
CCCEEECCCCCCCCCCEEECCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCC
AVTIRELSVEEAIELAHLGAKRLHPRTFEPVLRRHVDVRVLSLYDPKGNNTLISHGRRGP
CHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHCCCEEEEEEECCCCCCCEECCCCCCH
MLKAVALLNGLAIITVKGVGMVGTKGVAATVMESVTKVGANIYAISQPVSEISISIAVKS
HHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHEEEEEEEEC
SKADLVSNAIRNALEGKGISVETKIKDNVTAVSVVGRGLEELDFVGNVLSLFDNKPVLMV
CHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEE
SKGPLDQSLTVLTTNKASREIGRKIHEKIVEEMMQYYGEAK
ECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLVAKFGGSVLRDAEGYSASAKVVKECVGEGYKVVVVVSAMKGVTDSLLKVSGSLPKDW
CCCHHHHCCHHHHCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHCCCCCHHH
KKYVEALKRRHERVLQQLGSSERWFKELEKNVEELKNVLWALYILREHTPRTRDFVLSFG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ERLSTILMSAALEREGLNPIPLTPEKAGIFTDENYNEAVPLYDVIEREVPKRLEPFLEED
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
KVPVVTGFLGSTLEGSITTLGRGGSDLTATILGSSLKADEVRLYTDIDGIKTGNPKEFPD
CCCEEECCCCCCCCCCEEECCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCC
AVTIRELSVEEAIELAHLGAKRLHPRTFEPVLRRHVDVRVLSLYDPKGNNTLISHGRRGP
CHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHCCCEEEEEEECCCCCCCEECCCCCCH
MLKAVALLNGLAIITVKGVGMVGTKGVAATVMESVTKVGANIYAISQPVSEISISIAVKS
HHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHEEEEEEEEC
SKADLVSNAIRNALEGKGISVETKIKDNVTAVSVVGRGLEELDFVGNVLSLFDNKPVLMV
CHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEE
SKGPLDQSLTVLTTNKASREIGRKIHEKIVEEMMQYYGEAK
ECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]