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The map label for this gene is rlmH

Identifier: 15675944

GI number: 15675944

Start: 1849661

End: 1850140

Strand: Reverse

Name: rlmH

Synonym: SPy_2215

Alternate gene names: 15675944

Gene position: 1850140-1849661 (Counterclockwise)

Preceding gene: NA

Following gene: 15675940

Centisome position: 99.88

GC content: 32.08

Gene sequence:

>480_bases
ATGAAAGTGAAACTAATTTGTGTTGGAAAACTAAAAGAAAGATACCTCAAAGATGGTATTTCAGAATATCAAAAAAGACT
TTCCCGTTTTTGTCAATTCGAAATGATTGAGTTAACTGATGAAAGAACACCAGATAAAGCAAGTTTTGCTGATAATCAAT
TGATTATGAGTAAAGAAGCTCAGCGAATCCATAAAAAAATTGGGGAACGTGATTTTGTAATTGCATTAGCTATTGAAGGA
AAACAGTTTCCTTCAGAGACATTTAGTGAATTAATATCAGGGGTTACTGTTAAGGGATATTCAACTATCACGTTTATTAT
TGGTGGGAGTCTTGGATTAGATTCAATTATTAAAAAAAGAGCTAATATGCTAATGAGTTTTGGATTGTTAACCTTACCTC
ATCAGTTAATGCGTCTAGTCCTTACTGAGCAAATTTATCGTGCTTTTATGATTACACAAGGCAGTCCTTATCATAAGTAA

Upstream 100 bases:

>100_bases
TAATAAAGACCTTTGTACAAAGGTTTTTATTAGTAGTTGAAGAAAAATAACTTGTGGATAAATTGTGTAAAAAGTAATGT
TCTTGTGATACAATTAAGCT

Downstream 100 bases:

>100_bases
GAGAGTGTGAACTGGAATAACTAACTCTTACCACTTTATTAGATAATAGTTCGGTATAATTAAAAGCAAATATTAAAAAA
AGACGTACTAAATCCTATCT

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MKVKLICVGKLKERYLKDGISEYQKRLSRFCQFEMIELTDERTPDKASFADNQLIMSKEAQRIHKKIGERDFVIALAIEG
KQFPSETFSELISGVTVKGYSTITFIIGGSLGLDSIIKKRANMLMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK

Sequences:

>Translated_159_residues
MKVKLICVGKLKERYLKDGISEYQKRLSRFCQFEMIELTDERTPDKASFADNQLIMSKEAQRIHKKIGERDFVIALAIEG
KQFPSETFSELISGVTVKGYSTITFIIGGSLGLDSIIKKRANMLMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK
>Mature_159_residues
MKVKLICVGKLKERYLKDGISEYQKRLSRFCQFEMIELTDERTPDKASFADNQLIMSKEAQRIHKKIGERDFVIALAIEG
KQFPSETFSELISGVTVKGYSTITFIIGGSLGLDSIIKKRANMLMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=30.188679245283, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_STRP1 (Q99XH0)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_270118.1
- RefSeq:   YP_283227.1
- ProteinModelPortal:   Q99XH0
- SMR:   Q99XH0
- EnsemblBacteria:   EBSTRT00000001190
- EnsemblBacteria:   EBSTRT00000028250
- GeneID:   3571018
- GeneID:   901993
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_2215
- KEGG:   spz:M5005_Spy_1864
- GeneTree:   EBGT00050000028023
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- BioCyc:   SPYO160490:SPY2215-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1864-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 18185; Mature: 18185

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKLICVGKLKERYLKDGISEYQKRLSRFCQFEMIELTDERTPDKASFADNQLIMSKEA
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
QRIHKKIGERDFVIALAIEGKQFPSETFSELISGVTVKGYSTITFIIGGSLGLDSIIKKR
HHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCEECCCEEEEEEEECCCCHHHHHHHH
ANMLMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKVKLICVGKLKERYLKDGISEYQKRLSRFCQFEMIELTDERTPDKASFADNQLIMSKEA
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
QRIHKKIGERDFVIALAIEGKQFPSETFSELISGVTVKGYSTITFIIGGSLGLDSIIKKR
HHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCEECCCEEEEEEEECCCCHHHHHHHH
ANMLMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296