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The map label for this gene is recF

Identifier: 15675937

GI number: 15675937

Start: 1836947

End: 1838053

Strand: Direct

Name: recF

Synonym: SPy_2204

Alternate gene names: 15675937

Gene position: 1836947-1838053 (Clockwise)

Preceding gene: 15675936

Following gene: 15675941

Centisome position: 99.16

GC content: 37.22

Gene sequence:

>1107_bases
ATGTGGATTAAAGAGTTAGAGTTAAAACATTATCGCAATTATGACCACCTGCTTGCCTCATTTTCGTCGGGTTTAAATGT
CTTTATCGGTAATAATGCTCAAGGAAAAACCAATTTTCTCGAGGCAATCTATTTTCTATCACTTACTAGAAGTCATCGCA
CGAGGGCTGATAAAGAATTGATTCATTTTGATCACTCAACTGTTTCTCTTACTGGTAAGATTCAGCGTATTAGTGGTACT
GTTGATTTAGAAATCAATCTATCAGATAAAGGACGTGTGACAAAGATTAATGCGCTAAAGCAAGCAAAATTATCTGATTA
TATTGGAACGATGATGGTTGTGCTTTTTGCACCAGAAGATTTGCAACTAGTTAAGGGAGCACCTAGCCTTCGTCGCAAAT
TTATCGACATTGACCTTGGTCAAATTAAGCCTGTTTACTTATCTGAGTTATCCCATTATAACCACGTGCTTAAACAGCGT
AATAGCTATTTAAAAAGTGCCCAACAAATTGATGCCGCTTTTCTAGCTGTTCTAGATGAGCAATTAGCTAGCTATGGAGC
CCGTGTCATGGAACATCGTATTGATTTTATCAATGCGTTAGAGAAAGAAGCAAATACTCATCACCAAGCCATCTCAAATG
GTTTAGAAAGTCTCTCTCTCTCCTATCAGTCGTCAGTTGTCTTTGACAAAAAAACTAATATTTACCAACAATTTTTACAC
CAACTTGAAAAAAATCATCAGAAAGATTTCTTCCGTAAAAACACTAGCGTTGGTCCACATCGCGATGAATTAGCCTTTTA
CATTAACGGGATGAATGCTAACTTTGCTAGTCAAGGTCAGCACCGCAGCCTTATCCTTTCCTTAAAAATGGCTGAAGTTA
GTTTAATGAAGGCCTTGACTGGAGATAATCCCATTCTTTTACTAGACGATGTCATGAGCGAGCTAGACAATACACGACAA
ACAAAGTTACTTGAAACAGTAATTAAAGAAAATGTCCAAACCTTTATCACAACAACAAGTTTGGATCACCTGTCACAGTT
GCCAGAAGGTATCCGTATTTTTCACGTGACAAAAGGAACCGTTCAAATTGATTCTGATATTCACTAA

Upstream 100 bases:

>100_bases
AAACATAACAATCGTCAATCTACTACCAAAAAATCACTAAGAGCAACTAAGAAAACTAAAGGTAAACCAACTGCACCTGT
CCGCTTCCCAGGTATCTAGT

Downstream 100 bases:

>100_bases
TAACATCAAAAAAGGGTTCAGTTAAACTGAGCCTTTTTTGATATTATGTGGCGATTAAGATTTGACCACACCTAATAAAA
TAGCACCTACGATAAAACAA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIYFLSLTRSHRTRADKELIHFDHSTVSLTGKIQRISGT
VDLEINLSDKGRVTKINALKQAKLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
NSYLKSAQQIDAAFLAVLDEQLASYGARVMEHRIDFINALEKEANTHHQAISNGLESLSLSYQSSVVFDKKTNIYQQFLH
QLEKNHQKDFFRKNTSVGPHRDELAFYINGMNANFASQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQ
TKLLETVIKENVQTFITTTSLDHLSQLPEGIRIFHVTKGTVQIDSDIH

Sequences:

>Translated_368_residues
MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIYFLSLTRSHRTRADKELIHFDHSTVSLTGKIQRISGT
VDLEINLSDKGRVTKINALKQAKLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
NSYLKSAQQIDAAFLAVLDEQLASYGARVMEHRIDFINALEKEANTHHQAISNGLESLSLSYQSSVVFDKKTNIYQQFLH
QLEKNHQKDFFRKNTSVGPHRDELAFYINGMNANFASQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQ
TKLLETVIKENVQTFITTTSLDHLSQLPEGIRIFHVTKGTVQIDSDIH
>Mature_368_residues
MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIYFLSLTRSHRTRADKELIHFDHSTVSLTGKIQRISGT
VDLEINLSDKGRVTKINALKQAKLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
NSYLKSAQQIDAAFLAVLDEQLASYGARVMEHRIDFINALEKEANTHHQAISNGLESLSLSYQSSVVFDKKTNIYQQFLH
QLEKNHQKDFFRKNTSVGPHRDELAFYINGMNANFASQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQ
TKLLETVIKENVQTFITTTSLDHLSQLPEGIRIFHVTKGTVQIDSDIH

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=365, Percent_Identity=25.7534246575342, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_STRP1 (P0C0D1)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_270111.1
- RefSeq:   YP_283218.1
- ProteinModelPortal:   P0C0D1
- EnsemblBacteria:   EBSTRT00000001281
- EnsemblBacteria:   EBSTRT00000029149
- GeneID:   3571027
- GeneID:   901893
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_2204
- KEGG:   spz:M5005_Spy_1855
- GeneTree:   EBGT00050000028130
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   SPYO160490:SPY2204-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1855-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 41840; Mature: 41840

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIYFLSLTRSHRTRADKEL
CCCHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IHFDHSTVSLTGKIQRISGTVDLEINLSDKGRVTKINALKQAKLSDYIGTMMVVLFAPED
HEECCCEEEEEEEEEEECCEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHEEECCCC
LQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQRNSYLKSAQQIDAAFLAVLDE
HHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLASYGARVMEHRIDFINALEKEANTHHQAISNGLESLSLSYQSSVVFDKKTNIYQQFLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHH
QLEKNHQKDFFRKNTSVGPHRDELAFYINGMNANFASQGQHRSLILSLKMAEVSLMKALT
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEHHHHHHHHHHHC
GDNPILLLDDVMSELDNTRQTKLLETVIKENVQTFITTTSLDHLSQLPEGIRIFHVTKGT
CCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCE
VQIDSDIH
EEECCCCC
>Mature Secondary Structure
MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIYFLSLTRSHRTRADKEL
CCCHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IHFDHSTVSLTGKIQRISGTVDLEINLSDKGRVTKINALKQAKLSDYIGTMMVVLFAPED
HEECCCEEEEEEEEEEECCEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHEEECCCC
LQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQRNSYLKSAQQIDAAFLAVLDE
HHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLASYGARVMEHRIDFINALEKEANTHHQAISNGLESLSLSYQSSVVFDKKTNIYQQFLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHH
QLEKNHQKDFFRKNTSVGPHRDELAFYINGMNANFASQGQHRSLILSLKMAEVSLMKALT
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEHHHHHHHHHHHC
GDNPILLLDDVMSELDNTRQTKLLETVIKENVQTFITTTSLDHLSQLPEGIRIFHVTKGT
CCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCE
VQIDSDIH
EEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296