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The map label for this gene is tsf

Identifier: 15675850

GI number: 15675850

Start: 1747362

End: 1748402

Strand: Direct

Name: tsf

Synonym: SPy_2093

Alternate gene names: 15675850

Gene position: 1747362-1748402 (Clockwise)

Preceding gene: 15675849

Following gene: 15675854

Centisome position: 94.33

GC content: 42.07

Gene sequence:

>1041_bases
ATGGCAGAAATTACAGCTAAGCTTGTAAAAGAATTACGTGAAAAATCTGGTGCTGGCGTTATGGACGCTAAAAAAGCACT
TGTTGAAACAGATGGTGACATGGACAAAGCCGTTGAACTTCTTCGTGAAAAAGGAATGGCTAAAGCAGCTAAAAAAGCTG
ACCGTGTTGCCGCTGAAGGTTTAACAGGCGTTTACGTTCATGGTAACGTTGCTGCTGTTGTTGAAGTTAACGCTGAAACA
GACTTTGTTGCTAAAAATGCTCAATTCGTTGAATTGGTAAACGCAACTGCTAAAGTTATCGCTGAAGGCAAACCAGCTAA
CAACGATGAAGCACTTGCACTTGTTATGCCTTCAGGTGAAACTCTTGCTGAAGCTTACGTTAATGCAACTGCTACCATCG
GAGAAAAAATCTCATTCCGTCGTTTTGCTTTGATTGAAAAAGCAGATGAGCAACACTTCGGTGCTTACCAACATAATGGT
GGCCGTATCGGGGTTATCTCAGTTGTCGAAGGCGGCGACGATGCTCTTGCTAAACAAGTATCAATGCATATCGCTGCAAT
GAAACCAACTGTTCTTTCATACACTGAGCTTGATGCACAATTCATCAAAGATGAACTTGCTCAATTGAACCACGCTATCG
AACTTGACAACGAATCTCGTGCAATGGTTGATAAACCAGCTCTTCCATTCTTGAAATACGGTTCAAAAGCTCAATTATCA
GACGATGTGATTACTGCTGCTGAAGCAGACATCAAAGCTGAACTTGCTGCTGAAGGCAAACCAGAAAAGATCTGGGACAA
AATCATCCCAGGTAAAATGGACCGCTTCATGCTTGACAACACTAAAGTTGACCAAGCTTACACTCTTCTTGCACAAGTTT
ACATCATGGACGACAGCAAAACTGTTGAAGCTTACCTTGACTCAGTAAACGCTAAAGCGATTGCTTTTGCACGTTTTGAA
GTTGGTGAAGGTATCGAGAAAAAAGCTAACGACTTTGAATCAGAAGTTGCAGCTACTATGGCTGCTGCCCTTAACAACTA
A

Upstream 100 bases:

>100_bases
TTGTTTGTCTTTAACAATCGAAAACCAAACGGGGCAGAAAGCAAACCGCTCTGTCTCGTTTTTTGAAACCAAAACAAATA
TCATTTGGAGGATACTACAC

Downstream 100 bases:

>100_bases
TCACTTATTTCTGATAAGTCTAAAAAAGGCAACCTTCTTTCCTGAGGGTTGTCTTTTTGTTGTGTCAAAACGGACACCAA
TAAGGAAGCAAAAAACTGAT

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MAEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREKGMAKAAKKADRVAAEGLTGVYVHGNVAAVVEVNAET
DFVAKNAQFVELVNATAKVIAEGKPANNDEALALVMPSGETLAEAYVNATATIGEKISFRRFALIEKADEQHFGAYQHNG
GRIGVISVVEGGDDALAKQVSMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNESRAMVDKPALPFLKYGSKAQLS
DDVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRFMLDNTKVDQAYTLLAQVYIMDDSKTVEAYLDSVNAKAIAFARFE
VGEGIEKKANDFESEVAATMAAALNN

Sequences:

>Translated_346_residues
MAEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREKGMAKAAKKADRVAAEGLTGVYVHGNVAAVVEVNAET
DFVAKNAQFVELVNATAKVIAEGKPANNDEALALVMPSGETLAEAYVNATATIGEKISFRRFALIEKADEQHFGAYQHNG
GRIGVISVVEGGDDALAKQVSMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNESRAMVDKPALPFLKYGSKAQLS
DDVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRFMLDNTKVDQAYTLLAQVYIMDDSKTVEAYLDSVNAKAIAFARFE
VGEGIEKKANDFESEVAATMAAALNN
>Mature_345_residues
AEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREKGMAKAAKKADRVAAEGLTGVYVHGNVAAVVEVNAETD
FVAKNAQFVELVNATAKVIAEGKPANNDEALALVMPSGETLAEAYVNATATIGEKISFRRFALIEKADEQHFGAYQHNGG
RIGVISVVEGGDDALAKQVSMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNESRAMVDKPALPFLKYGSKAQLSD
DVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRFMLDNTKVDQAYTLLAQVYIMDDSKTVEAYLDSVNAKAIAFARFEV
GEGIEKKANDFESEVAATMAAALNN

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=230, Percent_Identity=28.2608695652174, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI291084498, Length=94, Percent_Identity=42.5531914893617, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI291084502, Length=97, Percent_Identity=41.2371134020619, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI291084500, Length=97, Percent_Identity=41.2371134020619, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786366, Length=338, Percent_Identity=36.6863905325444, Blast_Score=186, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17561440, Length=227, Percent_Identity=31.7180616740088, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19921466, Length=212, Percent_Identity=30.6603773584906, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_STRP1 (Q99XQ7)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_270024.1
- RefSeq:   YP_283144.1
- ProteinModelPortal:   Q99XQ7
- SMR:   Q99XQ7
- EnsemblBacteria:   EBSTRT00000000042
- EnsemblBacteria:   EBSTRT00000027566
- GeneID:   3571107
- GeneID:   901734
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_2093
- KEGG:   spz:M5005_Spy_1781
- GeneTree:   EBGT00050000028229
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- BioCyc:   SPYO160490:SPY2093-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1781-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 37228; Mature: 37096

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREKGMAKAAKKADRVAAEG
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCC
LTGVYVHGNVAAVVEVNAETDFVAKNAQFVELVNATAKVIAEGKPANNDEALALVMPSGE
CCEEEEECCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCH
TLAEAYVNATATIGEKISFRRFALIEKADEQHFGAYQHNGGRIGVISVVEGGDDALAKQV
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCEECCCCEEEEEEEEECCCHHHHHHH
SMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNESRAMVDKPALPFLKYGSKAQLS
HHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHEEECCCCCHHHCCCCCHHHHCCCCCCCC
DDVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRFMLDNTKVDQAYTLLAQVYIMDDSK
CHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCCHHHEECCCCHHHHHHHHEEEEEECCCH
TVEAYLDSVNAKAIAFARFEVGEGIEKKANDFESEVAATMAAALNN
HHHHHHHCCCCCEEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREKGMAKAAKKADRVAAEG
CCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCC
LTGVYVHGNVAAVVEVNAETDFVAKNAQFVELVNATAKVIAEGKPANNDEALALVMPSGE
CCEEEEECCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCH
TLAEAYVNATATIGEKISFRRFALIEKADEQHFGAYQHNGGRIGVISVVEGGDDALAKQV
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCEECCCCEEEEEEEEECCCHHHHHHH
SMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNESRAMVDKPALPFLKYGSKAQLS
HHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHEEECCCCCHHHCCCCCHHHHCCCCCCCC
DDVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRFMLDNTKVDQAYTLLAQVYIMDDSK
CHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCCHHHEECCCCHHHHHHHHEEEEEECCCH
TVEAYLDSVNAKAIAFARFEVGEGIEKKANDFESEVAATMAAALNN
HHHHHHHCCCCCEEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296