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The map label for this gene is def

Identifier: 15675756

GI number: 15675756

Start: 1626523

End: 1627137

Strand: Direct

Name: def

Synonym: SPy_1958

Alternate gene names: 15675756

Gene position: 1626523-1627137 (Clockwise)

Preceding gene: 15675755

Following gene: 15675770

Centisome position: 87.8

GC content: 40.33

Gene sequence:

>615_bases
ATGTCTGCACAAGATAAATTAATTAAACCTAGTCATCTGATTACCATGGATGACATTATTCGTGAAGGGAATCCAACTTT
AAGAGCTGTCGCCAAAGAAGTGAGCTTACCCTTGTGTGATGAGGATATCCTATTAGGAGAAAAGATGATGCAATTTTTGA
AGCATTCTCAAGATCCTGTAATGGCGGAAAAACTTGGTCTTAGGGCCGGTGTTGGTCTAGCAGCTCCTCAGATTGATGTG
TCAAAACGTATCATCGCTGTGCTCGTTCCTAATCTTCCTGATAAAGAAGGAAATCCTCCTAAAGAAGCCTATAGCTGGCA
AGAAGTCCTGTATAATCCTAAAATCGTTTCTCATTCTGTTCAAGATGCAGCCCTTTCTGATGGCGAAGGTTGTTTATCTG
TCGATCGTGTTGTTGAAGGGTATGTGGTTCGCCATGCTCGTGTTACAGTAGACTACTATGATAAAGAAGGACAACAGCAT
CGCATCAAATTAAAGGGATACAACGCTATTGTGGTTCAACATGAAATTGACCATATCAATGGTGTTTTGTTCTACGATCG
GATCAATGCTAAAAACCCCTTTGAAACCAAAGAAGAGCTCTTGATTTTAGACTAA

Upstream 100 bases:

>100_bases
CTTATAATAGCAATCACTCTAACCACTATCTGACTTTTCTTTAAAAAAGAAATGATTTGTGATATAGTAAATCCAGTTAA
AAAAAAGTAAAGGAGTAGAC

Downstream 100 bases:

>100_bases
CAAAAAAAGGCCCTTTAGCCTTTTTTTGTTAGTCTAAATAGCCGCTAAAAGAGCCTCTGCCTGAGATAGCAAGTTTGCTT
TTGTCTCTTTTGAAATCTCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MSAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDILLGEKMMQFLKHSQDPVMAEKLGLRAGVGLAAPQIDV
SKRIIAVLVPNLPDKEGNPPKEAYSWQEVLYNPKIVSHSVQDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQH
RIKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFETKEELLILD

Sequences:

>Translated_204_residues
MSAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDILLGEKMMQFLKHSQDPVMAEKLGLRAGVGLAAPQIDV
SKRIIAVLVPNLPDKEGNPPKEAYSWQEVLYNPKIVSHSVQDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQH
RIKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFETKEELLILD
>Mature_203_residues
SAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDILLGEKMMQFLKHSQDPVMAEKLGLRAGVGLAAPQIDVS
KRIIAVLVPNLPDKEGNPPKEAYSWQEVLYNPKIVSHSVQDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQHR
IKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFETKEELLILD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=183, Percent_Identity=30.6010928961749, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1789682, Length=129, Percent_Identity=31.7829457364341, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_STRP1 (P68771)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269930.1
- RefSeq:   YP_283032.1
- PDB:   2OS3
- PDBsum:   2OS3
- ProteinModelPortal:   P68771
- SMR:   P68771
- EnsemblBacteria:   EBSTRT00000001420
- EnsemblBacteria:   EBSTRT00000028366
- GeneID:   3571223
- GeneID:   901633
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1958
- KEGG:   spz:M5005_Spy_1669
- GeneTree:   EBGT00050000026595
- HOGENOM:   HBG665227
- OMA:   PVMGEKL
- ProtClustDB:   PRK00150
- BioCyc:   SPYO160490:SPY1958-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1669-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 22863; Mature: 22731

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDILLGEKMMQFLKHSQDPV
CCCCCCCCCCCCEEEHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCH
MAEKLGLRAGVGLAAPQIDVSKRIIAVLVPNLPDKEGNPPKEAYSWQEVLYNPKIVSHSV
HHHHHCCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHCCCC
QDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQHRIKLKGYNAIVVQHEIDHIN
HHHHCCCCCCCCHHHHHHHHHEEEEEEEEEEEECCCCCEEEEEEECCEEEEEEEECCCCC
GVLFYDRINAKNPFETKEELLILD
CEEEEEECCCCCCCCCCCCEEEEC
>Mature Secondary Structure 
SAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDILLGEKMMQFLKHSQDPV
CCCCCCCCCCCEEEHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCH
MAEKLGLRAGVGLAAPQIDVSKRIIAVLVPNLPDKEGNPPKEAYSWQEVLYNPKIVSHSV
HHHHHCCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHCCCC
QDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQHRIKLKGYNAIVVQHEIDHIN
HHHHCCCCCCCCHHHHHHHHHEEEEEEEEEEEECCCCCEEEEEEECCEEEEEEEECCCCC
GVLFYDRINAKNPFETKEELLILD
CEEEEEECCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296