Click here to switch to the map view.

The map label for this gene is pnpA [H]

Identifier: 15675748

GI number: 15675748

Start: 1618197

End: 1620329

Strand: Reverse

Name: pnpA [H]

Synonym: SPy_1946

Alternate gene names: 15675748

Gene position: 1620329-1618197 (Counterclockwise)

Preceding gene: 15675749

Following gene: 15675747

Centisome position: 87.47

GC content: 44.21

Gene sequence:

>2133_bases
ATGTCAAAACAAACCTTTACAACAACATTTGCAGGGAAACCCCTTGTTGTTGAAGTTGGTCAAGTCGCTAAGCAAGCCAA
TGGGGCAACCGTTGTTCGTTATGGCGACTCAACTGTCCTGACCGCAGCTGTTATGTCTAAGAAAATGGCAACGGGTGATT
TCTTCCCCCTTCAAGTTAATTACGAAGAAAAAATGTATGCCGCTGGTAAATTCCCAGGAGGTTTCATGAAACGTGAGGGA
CGCCCTTCAACAGATGCGACCTTGACAGCTCGCTTGATTGACCGCCCGATTCGTCCCATGTTTGCCGAAGGTTTTCGTAA
CGAAGTACAAGTGATTAACACTGTCCTTTCTTATGATGAAAATGCTAGCGCTCCAATGGCAGCGATGTTTGGCTCATCTC
TGGCCTTGTCAATCTCAGATATTCCATTTAATGGACCGATTGCAGGTGTTCAAGTTGGCTATATTGATGGCGAGTTCATC
ATTAACCCAGATAAAGAACAGATGGAAGCATCACTCTTAGAATTAACTGTTGCTGGTAGCAAAGAAGCCATCAATATGGT
TGAGTCTGGTGCCAAAGAATTATCAGAAGACATCATGCTTGAGGCTCTTTTAAAAGGTCACCAAGCTATTCAAGAATTAA
TTGCCTTCCAAGAACAAATCGTAGCAGTTGTTGGTAAAGAAAAAGCTGAGGTTGAACTGTTACAGGTTGATGTAGACTTA
CAAGCTGATATTGTGGCTAAGTACAATGCTCAACTGCAAAAAGCTGTTCAAGTTGAAGAGAAAAAAGCGCGTGAAGCAGC
CACAGAAGCCGTTAAAGAAATGGTTAAGGCTGAATACGAAGAGCGTTACGCAGAAGACGAAAACCTAGCAACCATTATGC
GTGACGTGGCAGAAATCCTTGAACAAATGGAGCATGCTGAGGTGCGCCGCCTCATTACAGAAGACAAGATTCGTCCTGAT
GGTCGTAAGATTGACGAAATTCGTCCGCTTGATGCTGTGGTTGACTTCTTGCCAAAAGTACATGGATCAGGTCTTTTTAC
GCGCGGTCAAACGCAGGCCTTGTCAGTTTTGACTTTGGCACCAATGGGAGAAACTCAAATCATTGATGGTCTGGCCCCAG
AGTACAAAAAACGCTTTTTGCATCACTACAATTTCCCGCAATATTCAGTTGGTGAAACGGGTCGTTACGGAGCTGCTGGT
CGTCGTGAAATTGGACACGGTGCCTTAGGTGAACGCGCTCTTGAACAAGTCTTGCCAAGCTTAGAAGAGTTTCCTTATGC
TATTCGTTTAGTTGCCGAAGTTCTAGAATCTAACGGTTCGTCTTCTCAGGCTTCTATCTGTGCTGGGACCCTTGCCCTAA
TGGCTGGTGGTGTGCCGATTAAAGCGCCTGTGGCAGGGATTGCCATGGGTCTCATTTCCGATGGGACGAATTATACAGTC
TTGACCGATATCCAAGGTCTTGAAGACCACTTTGGAGATATGGACTTTAAAGTGGCAGGAACTCGTGAAGGGATTACAGC
CCTTCAAATGGATATTAAGATTGCAGGAATTACCCCTCAAATCCTAGAAGAAGCTCTTGCACAAGCTAAAAAAGCTCGCT
TTGAAATCCTTGATGTGATTGAAGCAACTATCGCTGAACCACGTCCTGAGTTAGCCCCAACTGCACCGAAAATTGACACC
ATCAAGATTGACGTAGACAAAATCAAAGTGGTTATCGGTAAAGGTGGCGAAACTATTGACAAGATTATTGCCGAAACGGG
TGTCAAAATTGATATTGATGACGAAGGAAATGTCTCTATCTACTCAAGTGACCAAGCTGCCATTGACCGCACTAAAGAAA
TTATTGCAGGTCTTGTTCGTGAAGCTAAGGTAGGCGAAGTTTACCATGCTAAGGTTGTCCGTATTGAGAAATTTGGTGCC
TTTGTCAACCTCTTTGACAAGACAGATGCTCTTGTTCACATTTCTGAAATTGCCTGGACGAGAACGACCAACGTGTCAGA
TGTCCTTGAAGTGGGTGAAGACGTTGATGTTAAGGTCATTAAGATTGATGAGAAGGGTCGTGTGGATGCCTCAATGAAAG
CTTTGATTCCACGCCCACCAAAACCAGAGAAAAAAGAAGAAAAACATGACTAA

Upstream 100 bases:

>100_bases
GCGTGGCAGGTTGCTTTTTGTAGCAAAACAACGTATAACAACTGAATCTAAGCAGAGAACAGGTACCTCTTTACCCCTCT
GAAAATTAAAAGGAGAATAT

Downstream 100 bases:

>100_bases
ATCAAATGAATTAGATATTCGCTTAAGAGCTTTTATCAATGCGCCTGATAACTTTTTGGATAGCCTTGCGCTTGTCAACG
CTTTCCATAATTTTCCTGTC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGDSTVLTAAVMSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREG
RPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFI
INPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQEQIVAVVGKEKAEVELLQVDVDL
QADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVKAEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPD
GRKIDEIRPLDAVVDFLPKVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLAPEYKKRFLHHYNFPQYSVGETGRYGAAG
RREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGTNYTV
LTDIQGLEDHFGDMDFKVAGTREGITALQMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDT
IKIDVDKIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAIDRTKEIIAGLVREAKVGEVYHAKVVRIEKFGA
FVNLFDKTDALVHISEIAWTRTTNVSDVLEVGEDVDVKVIKIDEKGRVDASMKALIPRPPKPEKKEEKHD

Sequences:

>Translated_710_residues
MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGDSTVLTAAVMSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREG
RPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFI
INPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQEQIVAVVGKEKAEVELLQVDVDL
QADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVKAEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPD
GRKIDEIRPLDAVVDFLPKVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLAPEYKKRFLHHYNFPQYSVGETGRYGAAG
RREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGTNYTV
LTDIQGLEDHFGDMDFKVAGTREGITALQMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDT
IKIDVDKIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAIDRTKEIIAGLVREAKVGEVYHAKVVRIEKFGA
FVNLFDKTDALVHISEIAWTRTTNVSDVLEVGEDVDVKVIKIDEKGRVDASMKALIPRPPKPEKKEEKHD
>Mature_709_residues
SKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGDSTVLTAAVMSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGR
PSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFII
NPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQEQIVAVVGKEKAEVELLQVDVDLQ
ADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVKAEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPDG
RKIDEIRPLDAVVDFLPKVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLAPEYKKRFLHHYNFPQYSVGETGRYGAAGR
REIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGTNYTVL
TDIQGLEDHFGDMDFKVAGTREGITALQMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDTI
KIDVDKIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAIDRTKEIIAGLVREAKVGEVYHAKVVRIEKFGAF
VNLFDKTDALVHISEIAWTRTTNVSDVLEVGEDVDVKVIKIDEKGRVDASMKALIPRPPKPEKKEEKHD

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=716, Percent_Identity=35.7541899441341, Blast_Score=412, Evalue=1e-115,
Organism=Escherichia coli, GI145693187, Length=689, Percent_Identity=48.911465892598, Blast_Score=619, Evalue=1e-178,
Organism=Caenorhabditis elegans, GI115534063, Length=713, Percent_Identity=34.2215988779804, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI281362905, Length=697, Percent_Identity=37.1592539454806, Blast_Score=443, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24651641, Length=697, Percent_Identity=37.1592539454806, Blast_Score=443, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24651643, Length=697, Percent_Identity=37.1592539454806, Blast_Score=443, Evalue=1e-124,
Organism=Drosophila melanogaster, GI161079377, Length=661, Percent_Identity=36.4599092284418, Blast_Score=412, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77398; Mature: 77266

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGDSTVLTAAVMSKKMATGDFFPLQVN
CCCCEEEEEECCCCEEEEHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEC
YEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDE
CHHHEEECCCCCCCHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
NASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFIINPDKEQMEASLLELTVAGS
CCCCCHHHHCCCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHHHEEECCC
KEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQEQIVAVVGKEKAEVELLQVDVDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
QADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVKAEYEERYAEDENLATIMRDVAEIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EQMEHAEVRRLITEDKIRPDGRKIDEIRPLDAVVDFLPKVHGSGLFTRGQTQALSVLTLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCHHHEEEEEEC
PMGETQIIDGLAPEYKKRFLHHYNFPQYSVGETGRYGAAGRREIGHGALGERALEQVLPS
CCCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGTNYTV
HHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEE
LTDIQGLEDHFGDMDFKVAGTREGITALQMDIKIAGITPQILEEALAQAKKARFEILDVI
EEECCCHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EATIAEPRPELAPTAPKIDTIKIDVDKIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSI
HHHHCCCCCCCCCCCCCCEEEEEEHEEEEEEEECCCHHHHHHHHHCCCEEEECCCCCEEE
YSSDQAAIDRTKEIIAGLVREAKVGEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWT
EECCHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEHHHHHHHHHCCCCCEEEEHHHHEE
RTTNVSDVLEVGEDVDVKVIKIDEKGRVDASMKALIPRPPKPEKKEEKHD
CCCCHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGDSTVLTAAVMSKKMATGDFFPLQVN
CCCEEEEEECCCCEEEEHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEC
YEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDE
CHHHEEECCCCCCCHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
NASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFIINPDKEQMEASLLELTVAGS
CCCCCHHHHCCCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHHHEEECCC
KEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQEQIVAVVGKEKAEVELLQVDVDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
QADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVKAEYEERYAEDENLATIMRDVAEIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EQMEHAEVRRLITEDKIRPDGRKIDEIRPLDAVVDFLPKVHGSGLFTRGQTQALSVLTLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCHHHEEEEEEC
PMGETQIIDGLAPEYKKRFLHHYNFPQYSVGETGRYGAAGRREIGHGALGERALEQVLPS
CCCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGTNYTV
HHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEE
LTDIQGLEDHFGDMDFKVAGTREGITALQMDIKIAGITPQILEEALAQAKKARFEILDVI
EEECCCHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EATIAEPRPELAPTAPKIDTIKIDVDKIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSI
HHHHCCCCCCCCCCCCCCEEEEEEHEEEEEEEECCCHHHHHHHHHCCCEEEECCCCCEEE
YSSDQAAIDRTKEIIAGLVREAKVGEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWT
EECCHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEHHHHHHHHHCCCCCEEEEHHHHEE
RTTNVSDVLEVGEDVDVKVIKIDEKGRVDASMKALIPRPPKPEKKEEKHD
CCCCHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA