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The map label for this gene is cysS

Identifier: 15675744

GI number: 15675744

Start: 1614782

End: 1616125

Strand: Reverse

Name: cysS

Synonym: SPy_1941

Alternate gene names: 15675744

Gene position: 1616125-1614782 (Counterclockwise)

Preceding gene: 15675745

Following gene: 15675743

Centisome position: 87.24

GC content: 41.07

Gene sequence:

>1344_bases
ATGATTAAAATTTATGATACCATGACCCGTTCGCTCCGCAAGTTTGTACCTTTGACTGAAAATACAGTCAATATATACGT
TTGTGGACCGACGGTCTATAATTATATTCATATTGGAAATGCTAGATCAGCGGTCGCTTTTGACACCATTCGACGTTATT
TTGAGTATACTGGCTATCAGGTCAATTACATTTCCAATTTTACCGATGTCGATGATAAAATTATCAAGGCTGCTACTCAA
GCAGGTGTTTCTCCTAAAGAATTGTCAGATCGCTTTATTGCAGCTTTTATAGAAGATACCAAGGCACTTGGTGTTAAGCC
AGCCACACAAAATCCTCGTGTTATGGATTATATAGCAGAAATCATTTCATTTGTTGAAAGTCTCATTGAAAAAGATTTTG
CTTACGAAGCAGATGGAGATGTGTATTTCCGCGTGGAAAAGTCAGAGCATTATGCCAAGCTAGCTAATAAAACCCTGTCA
GAACTTGAAGTTGGAGCCAGTGGTCGAACAGATGCTGAGACAGCTTTAAAAGAAAATCCACTGGACTTTGCTCTTTGGAA
ATCAGCTAAGGCAGGTGAGGTTTCTTGGGATAGCCCCTGGGGGTTTGGTCGTCCAGGCTGGCACATTGAATGTTCTGTGA
TGGCTACTGAAATTCTTGGTGATACCATTGATATTCATGGTGGCGGAGCTGATTTGGAATTTCCTCATCATACCAATGAA
ATTGCCCAATCTGAGGCCAAAACAGGCAAGACTTTTGCTAACTATTGGATGCACAATGGGTTTGTCACTGTTGATAACGA
AAAAATGTCCAAGTCACTAGGTAATTTTGTGACCGTTCATGACATGTTACAAACCGTTGATGGTCAGGTTTTGCGATTCT
TTCTTGCGACACAACAGTACCGAAAACCAATTAACTTCACTGAAAAGACTATTCATGATGCGGAAATCAATCTGAAGTAT
CTTAAAAACACCTTGCAACAACCGCTGACAGAAACTGCAGATGAGCAAGAGCTAAAACAGTTTGTAATAGCTTTCCAAGA
CGCTATGGACGATGATTTCAATACAGCAAATGGGATTACCGTCGTATTTGACATGGCCAAGTGGATTAATTCTGGCTCCT
ATACAGAACCTGTTAAAAGCGCTTTTGAGAAAATGTTAGCTGTTTTTGGCATTATCTTTGAAGAAGAGGTGCTTGAGGTT
GACATTGAAGCCTTGATTGCCAAGCGACAAGAAGCACGCGCGAATCGTGATTTTGCCACAGCTGATGCCATTCGAGATCA
GTTAGCAGTTCAAGGCATCAAGCTGCTAGATACCAAAGATGGTGTGAGGTGGCTGCGTGACTAA

Upstream 100 bases:

>100_bases
TATTAATATTAAATTGTTGAAAAGGTGGAACAGCATTTCAGAAAAAGACTTAGGATATCAAGATGACAACTAATGCCAGA
CTATTGAATAAGGAGTTTAC

Downstream 100 bases:

>100_bases
TCCAGTTGATGTGAATTTGATTAATGGCATTGCCCTAGCCTTTGAAGGGGATGCGGTTTATTCCTACTATGTTCGTCGTC
ATCTCATTTTTCAAGGTAAA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MIKIYDTMTRSLRKFVPLTENTVNIYVCGPTVYNYIHIGNARSAVAFDTIRRYFEYTGYQVNYISNFTDVDDKIIKAATQ
AGVSPKELSDRFIAAFIEDTKALGVKPATQNPRVMDYIAEIISFVESLIEKDFAYEADGDVYFRVEKSEHYAKLANKTLS
ELEVGASGRTDAETALKENPLDFALWKSAKAGEVSWDSPWGFGRPGWHIECSVMATEILGDTIDIHGGGADLEFPHHTNE
IAQSEAKTGKTFANYWMHNGFVTVDNEKMSKSLGNFVTVHDMLQTVDGQVLRFFLATQQYRKPINFTEKTIHDAEINLKY
LKNTLQQPLTETADEQELKQFVIAFQDAMDDDFNTANGITVVFDMAKWINSGSYTEPVKSAFEKMLAVFGIIFEEEVLEV
DIEALIAKRQEARANRDFATADAIRDQLAVQGIKLLDTKDGVRWLRD

Sequences:

>Translated_447_residues
MIKIYDTMTRSLRKFVPLTENTVNIYVCGPTVYNYIHIGNARSAVAFDTIRRYFEYTGYQVNYISNFTDVDDKIIKAATQ
AGVSPKELSDRFIAAFIEDTKALGVKPATQNPRVMDYIAEIISFVESLIEKDFAYEADGDVYFRVEKSEHYAKLANKTLS
ELEVGASGRTDAETALKENPLDFALWKSAKAGEVSWDSPWGFGRPGWHIECSVMATEILGDTIDIHGGGADLEFPHHTNE
IAQSEAKTGKTFANYWMHNGFVTVDNEKMSKSLGNFVTVHDMLQTVDGQVLRFFLATQQYRKPINFTEKTIHDAEINLKY
LKNTLQQPLTETADEQELKQFVIAFQDAMDDDFNTANGITVVFDMAKWINSGSYTEPVKSAFEKMLAVFGIIFEEEVLEV
DIEALIAKRQEARANRDFATADAIRDQLAVQGIKLLDTKDGVRWLRD
>Mature_447_residues
MIKIYDTMTRSLRKFVPLTENTVNIYVCGPTVYNYIHIGNARSAVAFDTIRRYFEYTGYQVNYISNFTDVDDKIIKAATQ
AGVSPKELSDRFIAAFIEDTKALGVKPATQNPRVMDYIAEIISFVESLIEKDFAYEADGDVYFRVEKSEHYAKLANKTLS
ELEVGASGRTDAETALKENPLDFALWKSAKAGEVSWDSPWGFGRPGWHIECSVMATEILGDTIDIHGGGADLEFPHHTNE
IAQSEAKTGKTFANYWMHNGFVTVDNEKMSKSLGNFVTVHDMLQTVDGQVLRFFLATQQYRKPINFTEKTIHDAEINLKY
LKNTLQQPLTETADEQELKQFVIAFQDAMDDDFNTANGITVVFDMAKWINSGSYTEPVKSAFEKMLAVFGIIFEEEVLEV
DIEALIAKRQEARANRDFATADAIRDQLAVQGIKLLDTKDGVRWLRD

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=487, Percent_Identity=34.9075975359343, Blast_Score=252, Evalue=5e-67,
Organism=Homo sapiens, GI303304987, Length=348, Percent_Identity=33.9080459770115, Blast_Score=189, Evalue=6e-48,
Organism=Homo sapiens, GI21269877, Length=348, Percent_Identity=33.9080459770115, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI62240992, Length=348, Percent_Identity=33.9080459770115, Blast_Score=188, Evalue=8e-48,
Organism=Homo sapiens, GI10835051, Length=348, Percent_Identity=33.9080459770115, Blast_Score=188, Evalue=9e-48,
Organism=Escherichia coli, GI1786737, Length=466, Percent_Identity=44.6351931330472, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17509797, Length=246, Percent_Identity=39.4308943089431, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17509795, Length=246, Percent_Identity=39.4308943089431, Blast_Score=173, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6324082, Length=231, Percent_Identity=45.021645021645, Blast_Score=200, Evalue=4e-52,
Organism=Drosophila melanogaster, GI19922218, Length=507, Percent_Identity=32.9388560157791, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI19922364, Length=237, Percent_Identity=42.1940928270042, Blast_Score=192, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_STRP1 (Q99XZ9)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269918.1
- RefSeq:   YP_283018.1
- ProteinModelPortal:   Q99XZ9
- SMR:   Q99XZ9
- EnsemblBacteria:   EBSTRT00000000072
- EnsemblBacteria:   EBSTRT00000028668
- GeneID:   3571248
- GeneID:   901621
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1941
- KEGG:   spz:M5005_Spy_1655
- GeneTree:   EBGT00050000027085
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   SPYO160490:SPY1941-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1655-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 50491; Mature: 50491

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIYDTMTRSLRKFVPLTENTVNIYVCGPTVYNYIHIGNARSAVAFDTIRRYFEYTGYQ
CEEEHHHHHHHHHHHCCCCCCEEEEEEECCHHEEEEEECCCCCHHHHHHHHHHHHHCCEE
VNYISNFTDVDDKIIKAATQAGVSPKELSDRFIAAFIEDTKALGVKPATQNPRVMDYIAE
EEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
IISFVESLIEKDFAYEADGDVYFRVEKSEHYAKLANKTLSELEVGASGRTDAETALKENP
HHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
LDFALWKSAKAGEVSWDSPWGFGRPGWHIECSVMATEILGDTIDIHGGGADLEFPHHTNE
CCEEHCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCEEECCCCCCCCCCCCHHH
IAQSEAKTGKTFANYWMHNGFVTVDNEKMSKSLGNFVTVHDMLQTVDGQVLRFFLATQQY
HHHHHHHCCHHHHHHHHHCCEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RKPINFTEKTIHDAEINLKYLKNTLQQPLTETADEQELKQFVIAFQDAMDDDFNTANGIT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCE
VVFDMAKWINSGSYTEPVKSAFEKMLAVFGIIFEEEVLEVDIEALIAKRQEARANRDFAT
EEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ADAIRDQLAVQGIKLLDTKDGVRWLRD
HHHHHHHHHHCCHHEEECCCCHHHHCC
>Mature Secondary Structure
MIKIYDTMTRSLRKFVPLTENTVNIYVCGPTVYNYIHIGNARSAVAFDTIRRYFEYTGYQ
CEEEHHHHHHHHHHHCCCCCCEEEEEEECCHHEEEEEECCCCCHHHHHHHHHHHHHCCEE
VNYISNFTDVDDKIIKAATQAGVSPKELSDRFIAAFIEDTKALGVKPATQNPRVMDYIAE
EEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
IISFVESLIEKDFAYEADGDVYFRVEKSEHYAKLANKTLSELEVGASGRTDAETALKENP
HHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
LDFALWKSAKAGEVSWDSPWGFGRPGWHIECSVMATEILGDTIDIHGGGADLEFPHHTNE
CCEEHCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCEEECCCCCCCCCCCCHHH
IAQSEAKTGKTFANYWMHNGFVTVDNEKMSKSLGNFVTVHDMLQTVDGQVLRFFLATQQY
HHHHHHHCCHHHHHHHHHCCEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RKPINFTEKTIHDAEINLKYLKNTLQQPLTETADEQELKQFVIAFQDAMDDDFNTANGIT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCE
VVFDMAKWINSGSYTEPVKSAFEKMLAVFGIIFEEEVLEVDIEALIAKRQEARANRDFAT
EEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ADAIRDQLAVQGIKLLDTKDGVRWLRD
HHHHHHHHHHCCHHEEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296