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The map label for this gene is rpoE

Identifier: 15675708

GI number: 15675708

Start: 1571206

End: 1571781

Strand: Reverse

Name: rpoE

Synonym: SPy_1895

Alternate gene names: 15675708

Gene position: 1571781-1571206 (Counterclockwise)

Preceding gene: 15675709

Following gene: 15675707

Centisome position: 84.85

GC content: 36.46

Gene sequence:

>576_bases
TTGAAATTAGACGTATTTGCAGGACAAGAAAAAAGCGAGCTTTCCATGATTGAAGTGGCGCGTGCCATTTTGGAAGAACG
CGGTCGCGACAATGAGATGTACTTTAGCGATTTAGTCAATGAGATTCAAAATTACTTAGGAAAATCTGATGCAGGTATTC
GTCATGCATTACCATTTTTCTATACTGACTTAAATACGGATGGCTCATTTATTCCTCTAGGTGAAAACAAATGGGGACTT
CGTTCGTGGTATGCTATTGATGAAATTGATGAAGAAATTATTACTCTAGAAGAAGATGAAGATGGCGCACAAAAACGTAA
GAAAAAACGCGTTAATGCTTTCATGGATGGAGATGAAGACGCTATTGATTATAGAGATGATGATCCAGAAGATGAAGATT
TCACAGAAGAATCAGCTGAGGTTGAATATGATGAAGAAGATCCAGATGATGAAAAATCAGAAGTGGAGTCATATGATTCA
GAATTGAATGAAATTATCCCTGAAGATGATTTTGAAGAAGTTGATATCAATGAAGAAGACGAAGAAGATGAAGAAGATGA
AGAACCAGTTCTCTAA

Upstream 100 bases:

>100_bases
AAGATTTGTCAAGAAGAAGTGATATGAGTTGATAAAAGTTTTGACGAATTACTTTTTTTAGCGTAAAATGGTAGAGAATG
ATAAAATAAGGAGAACTGCC

Downstream 100 bases:

>100_bases
TGTCTTTAGTTCAGAAATAAAAATGTAATTATCATTTGACAAATTGTCTATTATAGTTTATATTATTATTCGGGCACCTC
TTTATGAGGTCAGGCAAAAG

Product: DNA-directed RNA polymerase subunit delta

Products: diphosphate; RNAn+1

Alternate protein names: RNAP delta factor

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQNYLGKSDAGIRHALPFFYTDLNTDGSFIPLGENKWGL
RSWYAIDEIDEEIITLEEDEDGAQKRKKKRVNAFMDGDEDAIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDS
ELNEIIPEDDFEEVDINEEDEEDEEDEEPVL

Sequences:

>Translated_191_residues
MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQNYLGKSDAGIRHALPFFYTDLNTDGSFIPLGENKWGL
RSWYAIDEIDEEIITLEEDEDGAQKRKKKRVNAFMDGDEDAIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDS
ELNEIIPEDDFEEVDINEEDEEDEEDEEPVL
>Mature_191_residues
MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQNYLGKSDAGIRHALPFFYTDLNTDGSFIPLGENKWGL
RSWYAIDEIDEEIITLEEDEDGAQKRKKKRVNAFMDGDEDAIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDS
ELNEIIPEDDFEEVDINEEDEEDEEDEEPVL

Specific function: Participates in both the initiation and recycling phases of transcription. In the presence of the delta subunit, RNAP displays an increased specificity of transcription, a decreased affinity for nucleic acids, and an increased efficiency of RNA synthesis

COG id: COG3343

COG function: function code K; DNA-directed RNA polymerase, delta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rpoE family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RPOE_STRP1 (P66719)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269882.1
- RefSeq:   YP_282974.2
- ProteinModelPortal:   P66719
- EnsemblBacteria:   EBSTRT00000000326
- EnsemblBacteria:   EBSTRT00000027613
- GeneID:   3571270
- GeneID:   902099
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1895
- KEGG:   spz:M5005_Spy_1611
- GeneTree:   EBGT00050000028344
- HOGENOM:   HBG535252
- OMA:   TDMNTDG
- ProtClustDB:   PRK02363
- BioCyc:   SPYO160490:SPY1895-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1611-MONOMER
- HAMAP:   MF_00357
- InterPro:   IPR007759
- PANTHER:   PTHR23405:SF1

Pfam domain/function: PF05066 RNA_pol_delta

EC number: 2.7.7.6

Molecular weight: Translated: 22265; Mature: 22265

Theoretical pI: Translated: 3.57; Mature: 3.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQNYLGKSDAGIRHALPFF
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCHHH
YTDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDGAQKRKKKRVNAFMDGDED
EEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCCC
AIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDSELNEIIPEDDFEEVDINEED
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
EEDEEDEEPVL
CCCCCCCCCCC
>Mature Secondary Structure
MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQNYLGKSDAGIRHALPFF
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCHHH
YTDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDGAQKRKKKRVNAFMDGDED
EEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCCC
AIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDSELNEIIPEDDFEEVDINEED
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
EEDEEDEEPVL
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nucleoside triphosphate; RNAn

Specific reaction: nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296