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The map label for this gene is rnjA [H]

Identifier: 15675695

GI number: 15675695

Start: 1556128

End: 1557810

Strand: Direct

Name: rnjA [H]

Synonym: SPy_1876

Alternate gene names: 15675695

Gene position: 1556128-1557810 (Clockwise)

Preceding gene: 15675694

Following gene: 15675710

Centisome position: 84.0

GC content: 40.05

Gene sequence:

>1683_bases
ATGACAAATATCAGTTTAAAACCTAATGAAGTTGGGGTGTTTGCCATTGGTGGTCTGGGAGAGATTGGGAAGAATACTTA
TGGTATTGAGTATCAAGACGAAATCATTATCGTTGATGCCGGTATCAAATTCCCAGAAGATGATCTTTTGGGAATTGATT
ACGTTATTCCAGACTATTCTTACATTGTCGATAACTTAGACCGGGTCAAAGCTCTTGTTATTACACATGGTCACGAAGAC
CACATTGGTGGTATTCCATTTCTTCTCAAACAGGCTAATATCCCTATCTACGCAGGACCTTTAGCACTTGCTCTTATCCG
TGGTAAATTGGAAGAACATGGTCTTTGGCGTGAAGCAACTGTTTACGAAATCAATCACAACACCGAGTTAACTTTTAAAA
ACATGAGCGTCACTTTCTTCAAGACTACTCATTCCATTCCAGAACCGGTCGGTATTGTTATCCACACCCCTCAAGGTAAG
ATTATCTGTACTGGTGATTTTAAATTTGACTTCACGCCAGTAGGAGATCCTGCAGATTTACAACGTATGGCAGCGCTTGG
TGAAGAAGGTGTCCTTTGCTTATTATCAGACTCAACTAATGCTGAAATCCCAACCTTTACTAACTCTGAAAAAGTTGTTG
GCCAATCCATTTTAAAAATTATCGAAGGTATTCATGGTCGTATTATTTTTGCTTCCTTCGCTTCAAACATCTACCGTTTG
CAACAGGCTGCAGAAGCTGCTGTTAAAACGGGACGTAAAATTGCCGTTTTTGGTCGTTCTATGGAAAAAGCAATTGTTAA
CGGTATTGAACTTGGCTATATTAAAGTTCCAAAAGGTACCTTCATTGAGCCAAGCGAACTTAAAAATCTTCATGCTAGTG
AAGTTTTAATTATGTGTACAGGTAGTCAAGGTGAGTCTATGGCAGCACTTGCTCGTATCGCTAATGGCACTCATCGTCAA
GTAACTTTACAGCCTGGTGATACTGTGATTTTCTCATCAAGTCCAATCCCTGGTAATACCACAAGTGTCAATAAATTAAT
CAATACCATCCAAGAAGCTGGTGTTGATGTTATTCATGGAAAAGTCAATAATATTCATACTTCTGGTCATGGAGGACAGC
AAGAACAAAAGCTCATGCTTAGTTTGATTAAACCAAAATATTTCATGCCAGTACATGGTGAGTATCGCATGCAAAAAGTT
CATGCAGGACTGGCGATGGATATTGGTATTCCAAAAGAAAATATCTTTATTATGGAAAATGGAGACGTTCTAGCTTTAAC
CAGTGACAGTGCGCGTATTGCTGGTCACTTTAATGCCCAAGACATCTACGTGGATGGTAATGGCATCGGGGATATTGGAG
CAGCCGTTTTACGGGACCGCCGTGATTTGTCAGAAGATGGTGTTGTCTTAGCTGTTGCAACTGTTGATTTTAACACACAA
ATGATTTTAGCTGGACCAGATATTCTGAGCCGTGGTTTCATTTATATGCGTGAGTCAGGTGACTTGATCCGCGAAAGCCA
ACGTGTGCTCTTTAACGCTATCCGTATTGCTTTAAAAAATAAGGATGCTAGCATCCAATCAGTTAACGGTGCTATCGTCA
ATGCTCTTCGTCCATTCTTGTATGAAAAGACTGAGCGTGAACCAATTATCATTCCGATGGTCTTAACACCTGATAAGCAC
TAA

Upstream 100 bases:

>100_bases
GATAACACCTATTATAACGTGGAATTTATCGAGCTACTTTCTGACAAACACCTCGATTACGAAAAAGAGACCGGTGTTTT
TGAATTAACGGAGTTCTAAG

Downstream 100 bases:

>100_bases
AATCAAATAAGACTCATTTCTTAATACTATCTGTCTATATCTTTAACATATCAGTTCTAGTTCAGATAATTATCAAACTA
AAACCAGTCCTCATAGAACA

Product: Zn-dependent hydrolase

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MTNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFPEDDLLGIDYVIPDYSYIVDNLDRVKALVITHGHED
HIGGIPFLLKQANIPIYAGPLALALIRGKLEEHGLWREATVYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGK
IICTGDFKFDFTPVGDPADLQRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSILKIIEGIHGRIIFASFASNIYRL
QQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKVPKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQ
VTLQPGDTVIFSSSPIPGNTTSVNKLINTIQEAGVDVIHGKVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVHGEYRMQKV
HAGLAMDIGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIGDIGAAVLRDRRDLSEDGVVLAVATVDFNTQ
MILAGPDILSRGFIYMRESGDLIRESQRVLFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDKH

Sequences:

>Translated_560_residues
MTNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFPEDDLLGIDYVIPDYSYIVDNLDRVKALVITHGHED
HIGGIPFLLKQANIPIYAGPLALALIRGKLEEHGLWREATVYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGK
IICTGDFKFDFTPVGDPADLQRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSILKIIEGIHGRIIFASFASNIYRL
QQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKVPKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQ
VTLQPGDTVIFSSSPIPGNTTSVNKLINTIQEAGVDVIHGKVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVHGEYRMQKV
HAGLAMDIGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIGDIGAAVLRDRRDLSEDGVVLAVATVDFNTQ
MILAGPDILSRGFIYMRESGDLIRESQRVLFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDKH
>Mature_559_residues
TNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFPEDDLLGIDYVIPDYSYIVDNLDRVKALVITHGHEDH
IGGIPFLLKQANIPIYAGPLALALIRGKLEEHGLWREATVYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGKI
ICTGDFKFDFTPVGDPADLQRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSILKIIEGIHGRIIFASFASNIYRLQ
QAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKVPKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQV
TLQPGDTVIFSSSPIPGNTTSVNKLINTIQEAGVDVIHGKVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVHGEYRMQKVH
AGLAMDIGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIGDIGAAVLRDRRDLSEDGVVLAVATVDFNTQM
ILAGPDILSRGFIYMRESGDLIRESQRVLFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDKH

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61098; Mature: 60967

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFPEDDLLGIDYVIPDYS
CCCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCEEEEEECCCHH
YIVDNLDRVKALVITHGHEDHIGGIPFLLKQANIPIYAGPLALALIRGKLEEHGLWREAT
HHHCCHHHEEEEEEECCCCCCCCCCCCEEEECCCCEEECHHHHHHHHHHHHHCCCCCEEE
VYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGKIICTGDFKFDFTPVGDPADL
EEEECCCCEEEEECEEEEEEEECCCCCCCCEEEEECCCCCEEEEECCEEEEECCCCCHHH
QRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSILKIIEGIHGRIIFASFASNIYRL
HHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHH
QQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKVPKGTFIEPSELKNLHASEVLIMCT
HHHHHHHHHCCCEEEEECCCHHHHHHCCEEEEEEECCCCCCCCHHHHCCCCCCEEEEEEE
GSQGESMAALARIANGTHRQVTLQPGDTVIFSSSPIPGNTTSVNKLINTIQEAGVDVIHG
CCCCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEC
KVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVHGEYRMQKVHAGLAMDIGIPKENIFIMEN
CCCEEEECCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHCCEEEEECCCCCCEEEEEC
GDVLALTSDSARIAGHFNAQDIYVDGNGIGDIGAAVLRDRRDLSEDGVVLAVATVDFNTQ
CCEEEEECCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCE
MILAGPDILSRGFIYMRESGDLIRESQRVLFNAIRIALKNKDASIQSVNGAIVNALRPFL
EEEECCCHHHCCEEEEECCCCHHHHHHHHHHHHHHEEEECCCCCEECCCHHHHHHHHHHH
YEKTEREPIIIPMVLTPDKH
HHCCCCCCEEEEEEECCCCC
>Mature Secondary Structure 
TNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFPEDDLLGIDYVIPDYS
CCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCEEEEEECCCHH
YIVDNLDRVKALVITHGHEDHIGGIPFLLKQANIPIYAGPLALALIRGKLEEHGLWREAT
HHHCCHHHEEEEEEECCCCCCCCCCCCEEEECCCCEEECHHHHHHHHHHHHHCCCCCEEE
VYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGKIICTGDFKFDFTPVGDPADL
EEEECCCCEEEEECEEEEEEEECCCCCCCCEEEEECCCCCEEEEECCEEEEECCCCCHHH
QRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSILKIIEGIHGRIIFASFASNIYRL
HHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHH
QQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKVPKGTFIEPSELKNLHASEVLIMCT
HHHHHHHHHCCCEEEEECCCHHHHHHCCEEEEEEECCCCCCCCHHHHCCCCCCEEEEEEE
GSQGESMAALARIANGTHRQVTLQPGDTVIFSSSPIPGNTTSVNKLINTIQEAGVDVIHG
CCCCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEC
KVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVHGEYRMQKVHAGLAMDIGIPKENIFIMEN
CCCEEEECCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHCCEEEEECCCCCCEEEEEC
GDVLALTSDSARIAGHFNAQDIYVDGNGIGDIGAAVLRDRRDLSEDGVVLAVATVDFNTQ
CCEEEEECCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCE
MILAGPDILSRGFIYMRESGDLIRESQRVLFNAIRIALKNKDASIQSVNGAIVNALRPFL
EEEECCCHHHCCEEEEECCCCHHHHHHHHHHHHHHEEEECCCCCEECCCHHHHHHHHHHH
YEKTEREPIIIPMVLTPDKH
HHCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]