Click here to switch to the map view.

The map label for this gene is grpE [H]

Identifier: 15675607

GI number: 15675607

Start: 1459341

End: 1459913

Strand: Reverse

Name: grpE [H]

Synonym: SPy_1761

Alternate gene names: 15675607

Gene position: 1459913-1459341 (Counterclockwise)

Preceding gene: 15675608

Following gene: 15675606

Centisome position: 78.81

GC content: 40.66

Gene sequence:

>573_bases
ATGGCAGTATTCAATAAGTTATTTAAAAGGAGGCACTCAGTGTCAGAAGAAATTAAAAAAGACGATTTACAAGAAGAAGT
GGAAGCCACTGAAACTGAAGAGACAGTTGAGGAAGTTATTGAAGAAACTCCTGAAAAATCAGAGTTAGAACTTGCAAACG
AACGTGCTGATGAGTTTGAAAATAAATACTTGCGAGCTCATGCAGAAATGCAGAATATACAGCGTCGCTCTAGTGAAGAA
CGTCAACAACTTCAACGCTATCGTTCTCAGGATCTGGCAAAAGCAATCTTACCATCGCTTGACAATCTAGAACGTGCCTT
GGCTGTTGAAGGGTTGACAGATGATGTTAAAAAAGGTTTAGAAATGACTCGTGATAGCCTTATTCAAGCACTAAAGGAAG
AAGGCGTAGAAGAAGTAGAAGTCGACTCGTTTGACCACAACTTTCATATGGCAGTTCAGACCCTCCCAGCCGATGATGAA
CATCCAGCCGATAGCATTGCAGAAGTCTTTCAAAAGGGATATAAATTGCACGAGCGTCTGCTAAGACCAGCGATGGTAGT
TGTTTATAACTAA

Upstream 100 bases:

>100_bases
GGTCTGTTAGTTTGGTAAACATTATTGGAAAGGTATTGGCTGCTAAATTAGGTGATTATTATCGATACCTAAATTCTAAC
CATTATGAAGTTCACTGATA

Downstream 100 bases:

>100_bases
GGTAAATGAGTTTTCGTTTTTGTCCGTAATGACAGTAAACTAGATAGCAAGTTAGAAGCTATTTCGCTTGCTGATTAAAC
TATAGTGATTGCTTAGAATT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETPEKSELELANERADEFENKYLRAHAEMQNIQRRSSEE
RQQLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
HPADSIAEVFQKGYKLHERLLRPAMVVVYN

Sequences:

>Translated_190_residues
MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETPEKSELELANERADEFENKYLRAHAEMQNIQRRSSEE
RQQLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
HPADSIAEVFQKGYKLHERLLRPAMVVVYN
>Mature_189_residues
AVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETPEKSELELANERADEFENKYLRAHAEMQNIQRRSSEER
QQLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDEH
PADSIAEVFQKGYKLHERLLRPAMVVVYN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=176, Percent_Identity=32.3863636363636, Blast_Score=75, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324806, Length=178, Percent_Identity=30.8988764044944, Blast_Score=76, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24653432, Length=170, Percent_Identity=32.3529411764706, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22055; Mature: 21924

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETPEKSELELANERADEFE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHH
EMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDEHPADSIAEVFQKGYKLHERL
HHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LRPAMVVVYN
HHHHHHHCCC
>Mature Secondary Structure 
AVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETPEKSELELANERADEFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHH
EMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDEHPADSIAEVFQKGYKLHERL
HHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LRPAMVVVYN
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12354221 [H]