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The map label for this gene is liaR [H]

Identifier: 15675498

GI number: 15675498

Start: 1343249

End: 1343890

Strand: Reverse

Name: liaR [H]

Synonym: SPy_1621

Alternate gene names: 15675498

Gene position: 1343890-1343249 (Counterclockwise)

Preceding gene: 15675499

Following gene: 15675497

Centisome position: 72.55

GC content: 39.88

Gene sequence:

>642_bases
ATGAGTAAGATAAAAGTGATATTGGTCGATGATCATGAAATGGTCCGCATGGGACTCAAGAGTTTTTTGAATTTACAAGC
TGATATTGATGTCGTTGGTGAGGCCTCTAATGGACGTGAAGGGGTTGATTTGGCATTGGCTTTGAAGCCAGATGTTTTGG
TTATGGATCTAGTGATGCCAGAGTTAGGCGGTGTTGAGGCAACTTTAGAAGTCCTAAAAAAATGGAAAGAGGCTAAGGTA
CTTGTGTTAACTTCCTATCTAGATAATGAAAAGATATACCCTGTCATTGATGCAGGAGCGAAGGGTTATATGTTAAAAAC
ATCGAGTGCGGCTGAAATTTTAAATGCCATTCGCAAGGTTTCAAAGGGAGAGTTAGCTATTGAAACAGAAGTTGACAAAA
AAATTAAGGCGCATGATCAACACCCTGACTTGCATGAGGAACTAACAGCGCGTGAGTATGACATTTTACACCTTTTAGCT
AAAGGGTATGATAATCAGACCATCGCTGATGAACTCTTTATTTCCTTAAAAACCGTCAAAACACATGTGTCCAATATTCT
AGCCAAGTTAGAAGTTGGTGACCGAACCCAAGCGGTTGTCTATGCTTTTCGACATCATTTAGTCCCCCAAGACGATAATT
AA

Upstream 100 bases:

>100_bases
GACCGTGTCAATGATTTAGCAGGAAACCTACATTTAATTAGTCAAAAAGGCAAAGGAGTTTCCATGGATATTAGACTGCC
GATAGTGAAGGGAGATGACG

Downstream 100 bases:

>100_bases
TGCTATAATATAGGGTATAGCAAATTGGAGGAAAGTATGGACGCAAAACTAAAATACAAAGCTAAAAAAATCAAAATGGT
GTTCTTTGATATTGATGATA

Product: putative two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSKIKVILVDDHEMVRMGLKSFLNLQADIDVVGEASNGREGVDLALALKPDVLVMDLVMPELGGVEATLEVLKKWKEAKV
LVLTSYLDNEKIYPVIDAGAKGYMLKTSSAAEILNAIRKVSKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLA
KGYDNQTIADELFISLKTVKTHVSNILAKLEVGDRTQAVVYAFRHHLVPQDDN

Sequences:

>Translated_213_residues
MSKIKVILVDDHEMVRMGLKSFLNLQADIDVVGEASNGREGVDLALALKPDVLVMDLVMPELGGVEATLEVLKKWKEAKV
LVLTSYLDNEKIYPVIDAGAKGYMLKTSSAAEILNAIRKVSKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLA
KGYDNQTIADELFISLKTVKTHVSNILAKLEVGDRTQAVVYAFRHHLVPQDDN
>Mature_212_residues
SKIKVILVDDHEMVRMGLKSFLNLQADIDVVGEASNGREGVDLALALKPDVLVMDLVMPELGGVEATLEVLKKWKEAKVL
VLTSYLDNEKIYPVIDAGAKGYMLKTSSAAEILNAIRKVSKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLAK
GYDNQTIADELFISLKTVKTHVSNILAKLEVGDRTQAVVYAFRHHLVPQDDN

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=209, Percent_Identity=35.8851674641148, Blast_Score=123, Evalue=8e-30,
Organism=Escherichia coli, GI1788222, Length=209, Percent_Identity=33.4928229665072, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1787473, Length=208, Percent_Identity=33.6538461538462, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1790102, Length=196, Percent_Identity=33.1632653061224, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1788712, Length=206, Percent_Identity=26.2135922330097, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1786747, Length=212, Percent_Identity=28.3018867924528, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI87082052, Length=110, Percent_Identity=35.4545454545455, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23694; Mature: 23563

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIKVILVDDHEMVRMGLKSFLNLQADIDVVGEASNGREGVDLALALKPDVLVMDLVMP
CCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEECCCEEEHHHHHC
ELGGVEATLEVLKKWKEAKVLVLTSYLDNEKIYPVIDAGAKGYMLKTSSAAEILNAIRKV
CCCCHHHHHHHHHHHHHCEEEEEEEECCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHC
SKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLAKGYDNQTIADELFISLKTVK
CCCCEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
THVSNILAKLEVGDRTQAVVYAFRHHLVPQDDN
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKIKVILVDDHEMVRMGLKSFLNLQADIDVVGEASNGREGVDLALALKPDVLVMDLVMP
CCEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEECCCEEEHHHHHC
ELGGVEATLEVLKKWKEAKVLVLTSYLDNEKIYPVIDAGAKGYMLKTSSAAEILNAIRKV
CCCCHHHHHHHHHHHHHCEEEEEEEECCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHC
SKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLAKGYDNQTIADELFISLKTVK
CCCCEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
THVSNILAKLEVGDRTQAVVYAFRHHLVPQDDN
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]