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The map label for this gene is gmuD [H]

Identifier: 15675482

GI number: 15675482

Start: 1316007

End: 1317449

Strand: Reverse

Name: gmuD [H]

Synonym: SPy_1599

Alternate gene names: 15675482

Gene position: 1317449-1316007 (Counterclockwise)

Preceding gene: 15675483

Following gene: 15675480

Centisome position: 71.12

GC content: 44.35

Gene sequence:

>1443_bases
ATGTTAGCATTTCCAAAAGAGTTTTGGTGGGGAGGAGCCACCTCAGGCCCTCAAAGTGAGGGGCGTTTTGCTAAGCAGCA
CCGCAATCTCTTTGATTATTGGTATGAAGAGGAGCCTGACTTGTTTTACGATTACGTGGGACCTGATACGGCTTCGGATG
CCTATCATCAGATTGAATCTGATTTGACCTTGTTAGCTTCACTAGGTCACAATTCTTACCGTACCTCCATTCAGTGGACA
CGTTTGATTGATGACTTTGAGCAAGCGACGATTAATCCTGATGGCCTTGCCTATTATAATCGAGTGATTGATGCTTGCTT
GGCCAATGGTATTCGTCCTGTTATTAATTTGCATCATTTTGACCTGCCAATTGCCCTCTATCAAGCCTATGGTGGCTGGG
AATCAAAGCATGTTGTGGACTTATTTGTCGCCTTTAGCAAGGTTTGTTTTGAGCAGTTTGGTGATCGGGTTAAGGATTGG
TTTGTCCACAATGAACCTATGGTCGTTGTGGAAGGCTCTTATTTAATGCAATTTCATTACCCTGCTATCGTGGATGGGAA
AAAAGCAGTCCAGGTCGCCTATAATCTAGCCCTAGCCACAGCCAAGGTTATTCAAGCTTACAGGCGAGGTCCAGCAGAAC
TTAGTGATGGGCGTATTGGAACCATTCTCAATCTGACGCCAGCCTACCCAGCTAGTCAATCAGAAGCGGATATGGCTGCT
GCGCATTTTGCAGAACTTTGGAACAATGACTTGTTTATGGAAGCTGCTGTTCATGGGAAATTCCCAGAAGAATTGGTTGC
GGTATTGAAAAAAGATGGGGTTTTGTGGCAATCAACGCCAGAAGAGTTGGCTCTTATTGCTGAGAATAGAGTGGATTATT
TGGGATTGAATTTTTACCACCCTAAGCGCGTGAAGGCTCCTGATGCCATTCCAGTGATTAGCCCATCTTGGAGTCCTGAA
TGGTATTATGATCCCTACCTGATGCCTGGCCGCCGCATGAACGTGGATAAGGGGTGGGAGATCTATCCTGAGGCTGTCTA
TGATATTGCCATCAAGATGCGAGACCATTACGACAATATTCCTTGGTTCTTATCTGAAAATGGTGTAGGGATTTCAGGAG
AAGACCGTTACCGTGATGAAACTGGTCAGATTCAAGATGATTATCGCATCCAATTTTTGAAAGAACATTTAACCTATCTC
CACAAAGGAATAGAAGCAGGCTCGAATTGTTTTGGTTACCATGTATGGACACCAATTGATGGCTGGTCTTGGCTCAATGC
CTACAAAAACCGCTATGGCCTTGTGGAAAATAATATCCATACCCAAGTGCGACGACCTAAAGCTTCAGCTTATTGGTTTA
AAAAAGTAGCTACTCATAACCGCTTGATTTCATTAGAAGTAATGGAGGAGTTTGGAGGCTCTGCTTCTCACCTCAGTGAT
TGA

Upstream 100 bases:

>100_bases
TCTACTATCAACAGCAAGATAAACTAACAGCTAATCAAGGTCAAGTTACCCAAGCAAGACCGGAAGACTATGCTTAGCAT
ACGAGCTAGAAAGGAATGAT

Downstream 100 bases:

>100_bases
ACGTGTGAACGTCTCCTTTGAGACAAGTGAATAATGATGTTACCAATGATTAAACAAGGGCCTTTTTCCTCATGTTAAGA
GGATCGAGGCTCTTGTATTT

Product: putative beta-glucosidase

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYDYVGPDTASDAYHQIESDLTLLASLGHNSYRTSIQWT
RLIDDFEQATINPDGLAYYNRVIDACLANGIRPVINLHHFDLPIALYQAYGGWESKHVVDLFVAFSKVCFEQFGDRVKDW
FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRRGPAELSDGRIGTILNLTPAYPASQSEADMAA
AHFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLWQSTPEELALIAENRVDYLGLNFYHPKRVKAPDAIPVISPSWSPE
WYYDPYLMPGRRMNVDKGWEIYPEAVYDIAIKMRDHYDNIPWFLSENGVGISGEDRYRDETGQIQDDYRIQFLKEHLTYL
HKGIEAGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASAYWFKKVATHNRLISLEVMEEFGGSASHLSD

Sequences:

>Translated_480_residues
MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYDYVGPDTASDAYHQIESDLTLLASLGHNSYRTSIQWT
RLIDDFEQATINPDGLAYYNRVIDACLANGIRPVINLHHFDLPIALYQAYGGWESKHVVDLFVAFSKVCFEQFGDRVKDW
FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRRGPAELSDGRIGTILNLTPAYPASQSEADMAA
AHFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLWQSTPEELALIAENRVDYLGLNFYHPKRVKAPDAIPVISPSWSPE
WYYDPYLMPGRRMNVDKGWEIYPEAVYDIAIKMRDHYDNIPWFLSENGVGISGEDRYRDETGQIQDDYRIQFLKEHLTYL
HKGIEAGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASAYWFKKVATHNRLISLEVMEEFGGSASHLSD
>Mature_480_residues
MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYDYVGPDTASDAYHQIESDLTLLASLGHNSYRTSIQWT
RLIDDFEQATINPDGLAYYNRVIDACLANGIRPVINLHHFDLPIALYQAYGGWESKHVVDLFVAFSKVCFEQFGDRVKDW
FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRRGPAELSDGRIGTILNLTPAYPASQSEADMAA
AHFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLWQSTPEELALIAENRVDYLGLNFYHPKRVKAPDAIPVISPSWSPE
WYYDPYLMPGRRMNVDKGWEIYPEAVYDIAIKMRDHYDNIPWFLSENGVGISGEDRYRDETGQIQDDYRIQFLKEHLTYL
HKGIEAGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASAYWFKKVATHNRLISLEVMEEFGGSASHLSD

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=491, Percent_Identity=27.4949083503055, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI32481206, Length=490, Percent_Identity=27.7551020408163, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI13273313, Length=482, Percent_Identity=28.2157676348548, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI28376633, Length=208, Percent_Identity=34.6153846153846, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI24497614, Length=439, Percent_Identity=26.1958997722096, Blast_Score=115, Evalue=8e-26,
Organism=Escherichia coli, GI1789070, Length=488, Percent_Identity=30.1229508196721, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI2367174, Length=492, Percent_Identity=29.4715447154472, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI2367270, Length=486, Percent_Identity=27.1604938271605, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17539390, Length=471, Percent_Identity=29.723991507431, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17552856, Length=470, Percent_Identity=29.5744680851064, Blast_Score=195, Evalue=4e-50,
Organism=Drosophila melanogaster, GI21356577, Length=489, Percent_Identity=26.5848670756646, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 55111; Mature: 55111

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYDYVGPDTASDAYHQIES
CCCCCCHHCCCCCCCCCCCCCHHHHHHCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHH
DLTLLASLGHNSYRTSIQWTRLIDDFEQATINPDGLAYYNRVIDACLANGIRPVINLHHF
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHEEECC
DLPIALYQAYGGWESKHVVDLFVAFSKVCFEQFGDRVKDWFVHNEPMVVVEGSYLMQFHY
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCEEEEEEC
PAIVDGKKAVQVAYNLALATAKVIQAYRRGPAELSDGRIGTILNLTPAYPASQSEADMAA
CEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHH
AHFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLWQSTPEELALIAENRVDYLGLNFYH
HHHHHHHCCCEEEEEHHCCCCHHHHHHHHHHCCCEECCCHHHHHHHHCCCCCEEECCCCC
PKRVKAPDAIPVISPSWSPEWYYDPYLMPGRRMNVDKGWEIYPEAVYDIAIKMRDHYDNI
CCCCCCCCCCCEECCCCCCCCEECCEECCCCCCCCCCCCCCCHHHHHHHHHEEHHCCCCC
PWFLSENGVGISGEDRYRDETGQIQDDYRIQFLKEHLTYLHKGIEAGSNCFGYHVWTPID
CEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
GWSWLNAYKNRYGLVENNIHTQVRRPKASAYWFKKVATHNRLISLEVMEEFGGSASHLSD
CHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCCC
>Mature Secondary Structure
MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYDYVGPDTASDAYHQIES
CCCCCCHHCCCCCCCCCCCCCHHHHHHCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHH
DLTLLASLGHNSYRTSIQWTRLIDDFEQATINPDGLAYYNRVIDACLANGIRPVINLHHF
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHEEECC
DLPIALYQAYGGWESKHVVDLFVAFSKVCFEQFGDRVKDWFVHNEPMVVVEGSYLMQFHY
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCEEEEEEC
PAIVDGKKAVQVAYNLALATAKVIQAYRRGPAELSDGRIGTILNLTPAYPASQSEADMAA
CEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHH
AHFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLWQSTPEELALIAENRVDYLGLNFYH
HHHHHHHCCCEEEEEHHCCCCHHHHHHHHHHCCCEECCCHHHHHHHHCCCCCEEECCCCC
PKRVKAPDAIPVISPSWSPEWYYDPYLMPGRRMNVDKGWEIYPEAVYDIAIKMRDHYDNI
CCCCCCCCCCCEECCCCCCCCEECCEECCCCCCCCCCCCCCCHHHHHHHHHEEHHCCCCC
PWFLSENGVGISGEDRYRDETGQIQDDYRIQFLKEHLTYLHKGIEAGSNCFGYHVWTPID
CEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
GWSWLNAYKNRYGLVENNIHTQVRRPKASAYWFKKVATHNRLISLEVMEEFGGSASHLSD
CHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]