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The map label for this gene is aroB

Identifier: 15675468

GI number: 15675468

Start: 1299152

End: 1300225

Strand: Direct

Name: aroB

Synonym: SPy_1577

Alternate gene names: 15675468

Gene position: 1299152-1300225 (Clockwise)

Preceding gene: 15675467

Following gene: 15675481

Centisome position: 70.13

GC content: 42.27

Gene sequence:

>1074_bases
ATGCCACAAACACTTCATGTCCATTCTCGGGTCAAGGATTATGATATCCTGTTTACCGACCATGTTTTAAAAACCTTAGC
AGATTGCCTTGGTGAGCGCAAGCAGCGTAAACTCTTGTTTATCACCGATCAAACGGTCTACCACTTGTATCAGACTCTCT
TTGAAGAATTTGCTCAGCAATACAACGCTTTTGTCCATGTCTGCCCTCCGGGAGGTCAATCCAAATCCTTGGAACGTGTC
AGTGCTATCTACGACCAGCTCATCGCTGAAAATTTTTCCAAAAAAGATATGATTGTTACTATTGGTGGTGGTGTTGTTGG
GGATCTGGGTGGTTTTGTCGCTGCAACCTATTACCGTGGTATTCCCTACATTCAAATCCCAACGACTTTACTTAGTCAGG
TGGATAGCAGTATCGGGGGCAAAGTTGGAGTCCATTTTAAAGGTCTAACCAATATGATTGGCAGCATTTACCCTCCAGAA
GCTATCATTATTTCAACCACCTTTTTGGAGACCTTACCGCAACGGGAATTCTCCTGCGGCATCAGTGAGATGCTCAAAAT
CGGCTTCATTCATGATAGACCGCTTTTTCAGCAGTTACGTGATTTTCAAAAAGAAACCGATAAGCAGGGACTTGAACGTC
TGATTTACCAATCCATTTCCAATAAAAAACGGATTGTTGAACAAGATGAATTTGAAAATGGTTTGCGCATGTCTTTAAAT
TTTGGGCATACCTTGGGCCACGCGATTGAATCGCTCTGCCACCATGATTTTTACCATCACGGTGAAGCCATTGCTATCGG
TATGGTGGTTGATGCTAAACTGGCTGTTTCCAAAGGACTCTTGCCAAAAGAGGACTTGGATAGCCTCCTTCAAGTTTTTG
AACGTTACCAGTTACCAACAACACTTGAAAGAGCTGATGTCTCTGCGACATCCCTCTTTGACGTCTTTAAAACAGATAAG
AAGAATTCTGAGCAACATATTATCTTTATATTACCCACAGAAACCGGTTTTACCACCCTAGCCATTAACAAAGACGATCA
CCAATTTGTCGAGAAGCTCGACAGTTTATTATAA

Upstream 100 bases:

>100_bases
TATAAGCAATTAGAACAGTTGGGGCAGGAACTTTGGCAAGATTAAGCTCTAAATATCACTGCGTTATCTCTGTTACCTAG
TACTGATGAAAGGAGTGCTT

Downstream 100 bases:

>100_bases
ATAGTGACTCCACTCTAGCTAGTTAAGCTTGCTAAGAGAATAACGGTATCCTGGAACTCCTAAAACATGTTACTACAGAC
AAGTCTTGATAACAATATAA

Product: 3-dehydroquinate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MPQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQTVYHLYQTLFEEFAQQYNAFVHVCPPGGQSKSLERV
SAIYDQLIAENFSKKDMIVTIGGGVVGDLGGFVAATYYRGIPYIQIPTTLLSQVDSSIGGKVGVHFKGLTNMIGSIYPPE
AIIISTTFLETLPQREFSCGISEMLKIGFIHDRPLFQQLRDFQKETDKQGLERLIYQSISNKKRIVEQDEFENGLRMSLN
FGHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAVSKGLLPKEDLDSLLQVFERYQLPTTLERADVSATSLFDVFKTDK
KNSEQHIIFILPTETGFTTLAINKDDHQFVEKLDSLL

Sequences:

>Translated_357_residues
MPQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQTVYHLYQTLFEEFAQQYNAFVHVCPPGGQSKSLERV
SAIYDQLIAENFSKKDMIVTIGGGVVGDLGGFVAATYYRGIPYIQIPTTLLSQVDSSIGGKVGVHFKGLTNMIGSIYPPE
AIIISTTFLETLPQREFSCGISEMLKIGFIHDRPLFQQLRDFQKETDKQGLERLIYQSISNKKRIVEQDEFENGLRMSLN
FGHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAVSKGLLPKEDLDSLLQVFERYQLPTTLERADVSATSLFDVFKTDK
KNSEQHIIFILPTETGFTTLAINKDDHQFVEKLDSLL
>Mature_356_residues
PQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQTVYHLYQTLFEEFAQQYNAFVHVCPPGGQSKSLERVS
AIYDQLIAENFSKKDMIVTIGGGVVGDLGGFVAATYYRGIPYIQIPTTLLSQVDSSIGGKVGVHFKGLTNMIGSIYPPEA
IIISTTFLETLPQREFSCGISEMLKIGFIHDRPLFQQLRDFQKETDKQGLERLIYQSISNKKRIVEQDEFENGLRMSLNF
GHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAVSKGLLPKEDLDSLLQVFERYQLPTTLERADVSATSLFDVFKTDKK
NSEQHIIFILPTETGFTTLAINKDDHQFVEKLDSLL

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; second step. [C]

COG id: COG0337

COG function: function code E; 3-dehydroquinate synthetase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dehydroquinate synthase family

Homologues:

Organism=Escherichia coli, GI1789791, Length=330, Percent_Identity=34.5454545454545, Blast_Score=177, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6320332, Length=286, Percent_Identity=33.9160839160839, Blast_Score=159, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROB_STRP1 (Q99YR3)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269642.1
- RefSeq:   YP_282661.1
- ProteinModelPortal:   Q99YR3
- SMR:   Q99YR3
- EnsemblBacteria:   EBSTRT00000001267
- EnsemblBacteria:   EBSTRT00000027455
- GeneID:   3571612
- GeneID:   901826
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1577
- KEGG:   spz:M5005_Spy_1298
- GeneTree:   EBGT00050000027456
- HOGENOM:   HBG632303
- OMA:   CEIKAEV
- ProtClustDB:   PRK00002
- BioCyc:   SPYO160490:SPY1577-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1298-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00110
- InterPro:   IPR016303
- InterPro:   IPR002658
- InterPro:   IPR016037
- PANTHER:   PTHR21090:SF1
- PIRSF:   PIRSF001455
- TIGRFAMs:   TIGR01357

Pfam domain/function: PF01761 DHQ_synthase

EC number: =4.2.3.4

Molecular weight: Translated: 40360; Mature: 40229

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQTVYHLYQTLFEEFAQQ
CCCCEEHHHCCCCHHEEHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHH
YNAFVHVCPPGGQSKSLERVSAIYDQLIAENFSKKDMIVTIGGGVVGDLGGFVAATYYRG
CCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHCC
IPYIQIPTTLLSQVDSSIGGKVGVHFKGLTNMIGSIYPPEAIIISTTFLETLPQREFSCG
CCEEEEHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCEEEEEHHHHHHHCCHHHHCC
ISEMLKIGFIHDRPLFQQLRDFQKETDKQGLERLIYQSISNKKRIVEQDEFENGLRMSLN
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHCCCCEEEEE
FGHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAVSKGLLPKEDLDSLLQVFERYQLPT
CCHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
TLERADVSATSLFDVFKTDKKNSEQHIIFILPTETGFTTLAINKDDHQFVEKLDSLL
HHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure 
PQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQTVYHLYQTLFEEFAQQ
CCCEEHHHCCCCHHEEHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHH
YNAFVHVCPPGGQSKSLERVSAIYDQLIAENFSKKDMIVTIGGGVVGDLGGFVAATYYRG
CCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHCC
IPYIQIPTTLLSQVDSSIGGKVGVHFKGLTNMIGSIYPPEAIIISTTFLETLPQREFSCG
CCEEEEHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCEEEEEHHHHHHHCCHHHHCC
ISEMLKIGFIHDRPLFQQLRDFQKETDKQGLERLIYQSISNKKRIVEQDEFENGLRMSLN
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHCCCCEEEEE
FGHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAVSKGLLPKEDLDSLLQVFERYQLPT
CCHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
TLERADVSATSLFDVFKTDKKNSEQHIIFILPTETGFTTLAINKDDHQFVEKLDSLL
HHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296