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The map label for this gene is ddl

Identifier: 15675338

GI number: 15675338

Start: 1179976

End: 1181022

Strand: Reverse

Name: ddl

Synonym: SPy_1421

Alternate gene names: 15675338

Gene position: 1181022-1179976 (Counterclockwise)

Preceding gene: 15675339

Following gene: 15675337

Centisome position: 63.75

GC content: 39.45

Gene sequence:

>1047_bases
ATGTCCAAACAAACGTTAGTTCTATTATACGGAGGCCGCTCAGCAGAACGTGAAGTCTCAGTGCTTTCAGCAGAGAGTGT
GATGCGAGCAGTCAACTATGATAAGTTCTTAGTAAAAACTTATTTTATTACTCAGATGGGCCAATTTATCAAAACTCAGC
AATTTTCAGAAAAGCCATCGGAATCAGAACGCTTGATGACAAATGAGACGATAGAGCTAACTCAAAAAATAAAACCAAGT
GATATCTATGAAGAAGGTGCAGTTGTATTTCCTGTATTACATGGACCTATGGGAGAAGATGGCTCTATCCAAGGTTTCTT
AGAAGTGCTTAGAATGCCATATATAGGGACTAATGTCATGTCTTCTAGCATAGCTATGGATAAAATTACTACTAAACGTG
TCTTAGAATCAATTGGAATTCCACAAGTTGCCTATACTGTTTATATTGACGGACAAGATTTGGAGGCTTGTTTAGTTGAA
ACTTTAGCAAGACTTACTTTTCCTATCTTCGTAAAACCAGCTAATATGGGTTCTTCTGTGGGGATTTCCAAAGCCCAAAC
AAAAGTAGAATTAAGAAAAGCGATTCAGTTAGCCCTGACCTATGACAGTCGTGTCTTGATTGAACAAGGCGTGGTTGCAA
GAGAAATTGAAGTTGGTCTCCTTGGAAATGATAAGGTTAAATCAACTCTACCAGGAGAAGTAATCAAGGACGTTGATTTT
TACGACTACCAGGCAAAATATGTTGACAATAAGATTACGATGGCCATTCCAGCAGATGTAGACCAGAGTATTGTGACAGA
AATGCGTTCTTATGCTGAAGTAGCCTTTAAAGCCTTAGGTGGTTGTGGGTTGTCCCGTTGTGACTTTTTCTTGACCCAAG
ATGGACAGGTTTACCTTAATGAATTAAACACCATGCCAGGCTTTACCCAATGGTCTATGTATCCACTCTTATGGGAAAAT
ATGGGATTGGCTTATCCAGATTTGATTGAAGAGCTGGTGACACTGGCACAAGAAATGTTTGACCAGCGTGAGAGCCACCT
GATTTAA

Upstream 100 bases:

>100_bases
TGATCATGTTGGTGCCTTTACTCGCAAAGAGAGGTTTTGAAAAGGCTACATCTTTTTGTTATAATAAAAAAACATTCAAT
TCAGATAAGAAAGGTTAGCT

Downstream 100 bases:

>100_bases
ACCTATGAGATGAATAAACATTGACTTGGATGGAACGGCAAACATCGTCAAGATGTGACGTTCGTCCTTTTTTTGTTGCT
CTTTATAGTAGAGTCATTAA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFITQMGQFIKTQQFSEKPSESERLMTNETIELTQKIKPS
DIYEEGAVVFPVLHGPMGEDGSIQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVE
TLARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEVGLLGNDKVKSTLPGEVIKDVDF
YDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAFKALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWEN
MGLAYPDLIEELVTLAQEMFDQRESHLI

Sequences:

>Translated_348_residues
MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFITQMGQFIKTQQFSEKPSESERLMTNETIELTQKIKPS
DIYEEGAVVFPVLHGPMGEDGSIQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVE
TLARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEVGLLGNDKVKSTLPGEVIKDVDF
YDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAFKALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWEN
MGLAYPDLIEELVTLAQEMFDQRESHLI
>Mature_347_residues
SKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFITQMGQFIKTQQFSEKPSESERLMTNETIELTQKIKPSD
IYEEGAVVFPVLHGPMGEDGSIQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVET
LARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEVGLLGNDKVKSTLPGEVIKDVDFY
DYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAFKALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWENM
GLAYPDLIEELVTLAQEMFDQRESHLI

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=360, Percent_Identity=35.5555555555556, Blast_Score=244, Evalue=8e-66,
Organism=Escherichia coli, GI1786280, Length=340, Percent_Identity=32.3529411764706, Blast_Score=160, Evalue=9e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_STRP1 (Q99Z34)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269512.1
- RefSeq:   YP_282521.1
- ProteinModelPortal:   Q99Z34
- SMR:   Q99Z34
- EnsemblBacteria:   EBSTRT00000000353
- EnsemblBacteria:   EBSTRT00000028346
- GeneID:   3571743
- GeneID:   901481
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1421
- KEGG:   spz:M5005_Spy_1158
- GeneTree:   EBGT00050000027793
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   SPYO160490:SPY1421-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1158-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 38969; Mature: 38838

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFITQMGQFIKTQQFSEKPS
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
ESERLMTNETIELTQKIKPSDIYEEGAVVFPVLHGPMGEDGSIQGFLEVLRMPYIGTNVM
CHHHHHCCCHHHHHHCCCCCCHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHH
SSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVETLARLTFPIFVKPANMGSSV
HHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCC
GISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEVGLLGNDKVKSTLPGEVIKDVDF
CCHHHHHHHHHHHHHHHHEECCCEEHHHCCCEEEEEEEEECCCCCCCCCCCHHHHHCCCC
YDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAFKALGGCGLSRCDFFLTQDGQVYLN
CCCCCEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHEE
ELNTMPGFTQWSMYPLLWENMGLAYPDLIEELVTLAQEMFDQRESHLI
HHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFITQMGQFIKTQQFSEKPS
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
ESERLMTNETIELTQKIKPSDIYEEGAVVFPVLHGPMGEDGSIQGFLEVLRMPYIGTNVM
CHHHHHCCCHHHHHHCCCCCCHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHH
SSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVETLARLTFPIFVKPANMGSSV
HHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCC
GISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEVGLLGNDKVKSTLPGEVIKDVDF
CCHHHHHHHHHHHHHHHHEECCCEEHHHCCCEEEEEEEEECCCCCCCCCCCHHHHHCCCC
YDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAFKALGGCGLSRCDFFLTQDGQVYLN
CCCCCEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHEE
ELNTMPGFTQWSMYPLLWENMGLAYPDLIEELVTLAQEMFDQRESHLI
HHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296