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The map label for this gene is kup

Identifier: 15675333

GI number: 15675333

Start: 1171773

End: 1173773

Strand: Reverse

Name: kup

Synonym: SPy_1414

Alternate gene names: 15675333

Gene position: 1173773-1171773 (Counterclockwise)

Preceding gene: 15675334

Following gene: 15675330

Centisome position: 63.36

GC content: 39.98

Gene sequence:

>2001_bases
ATGTCTGATAGCCATCTTACTGCCTTTGACAAAGCTTCAAAAGCAGGTTTTATTATTGCTTTAGGTATTGTTTATGGAGA
TATTGGAACAAGCCCCCTTTATACGATACAGTCTCTAGTTGAAAATCAAGGAGGGGTTAATCAGGTTTCTGAATCCTTTA
TTTTAGGATCAATCTCTTTAATTATTTGGACACTGACCTTAATCACAACAATCAAGTACGTTTTGATTGCTCTAAAAGCG
GATAACCACCATGAAGGTGGGATTTTTTCTTTATTCACCTTGGTACGTAAAATGTCACCGTGGCTCATTATTCCGGCGAT
GATTGGAGGTGCAACACTTTTGTCTGATGGGGCTCTTACACCTGCGGTAACAGTTACTTCAGCTATTGAGGGCTTGAAAG
CCGTTCCTGGTCTGAGTCACATCTACCAAAATCAAACCAATGTCATTATCACAACTCTAGTGATTTTAATAGTCTTGTTT
GGTATTCAGCGGTTTGGAACAGGTTTTATTGGTAAAATTTTTGGACCAGTTATGTTTATCTGGTTTAGTTTTCTAGGTGT
TAGTGGTTTTTTTAATACGTTGGGGCATCTGGAAATTTTCAAGGCTATCAATCCTTACTATGCTCTGCATTTGCTGTTTA
GTCCTGAAAACCATCGTGGGATTTTTATCTTGGGGTCCATTTTTCTTGCGACAACAGGAGCTGAAGCTCTTTATTCGGAT
TTAGGACATGTTGGTCGAGGAAATATTTACGTGAGCTGGCCCTTTGTTAAGATGTGTATTGTGTTGTCTTACTGTGGCCA
GGCTGCGTGGATTTTAGCGAATAAACACTCAGGTATTGAACTAAACCCTTTCTTTGCTAGTGTGCCATCACAATTAAGAG
TTTACCTTGTTAGTTTGGCAACCTTGGCAGCTATTATTGCCTCACAGGCTCTTATTTCAGGCTCCTTTACCTTGGTTTCA
GAGGCCATGCGTTTGAAAATTTTCCCTTTGTTTCGTGTGACTTACCCAGGGGCAAATTTGGGGCAATTGTATATTCCTGT
CATTAACTGGATTTTATTTGCAGTGACCTCTTGTACTGTTTTGGCTTTTAGAACATCAGCCCACATGGAGGCAGCTTATG
GTCTTGCTATTACCATCACCATGTTGATGACAACAATCCTCTTAAAGTATTACTTAATCAAGAAAGGAACGAGACCCATC
TTGGCTCATTTAGTGATGGCTTTCTTTGCCCTTGTAGAATTCATTTTCTTCTTGGCTTCAGCTATCAAATTTATGCACGG
TGGCTATGCGGTTGTTATCTTGGCACTTGCAATTGTCTTTGTCATGTTTATCTGGCATGCGGGCACACGGATTGTTTTCA
AGTATGTGAAATCTCTTAATTTGAATGATTACAAGGAGCAGATTAAACAACTTCGTGACGACGTTTGTTTTGACCTCTAC
CAAACAAATGTTGTCTACCTATCCAATCGTATGCAGGATCATATGATTGACCGGTCTATTTTATACTCCATTTTAGACAA
GCGGCCAAAACGCGCTCAAGTTTATTGGTTTGTAAATGTCCAGGTAACTGATGAACCTTATACAGCTAAGTACAAGGTAG
ATATGATGGGAACGGATTATATGGTTCGAGTCAACCTTTATCTTGGTTTTAGAATGCCACAGACTGTGCCACGCTATTTG
CGCACGATTGTTCAGGATTTGATGGAAAGTGGCCGTTTACCCAAACAAGAACAGGAATATACCATCACACCTGGTCGTGA
TGTTGGTGATTTTCGCTTTGTTTTAATAGAAGAACGGGTCTCCAATGCACGTCAATTGAGTAACTTTGAACGCTTTATTA
TGCAGACTAAGGCAAGCATTAAACATGTTACCGCTAGTCCAATGCGTTGGTTTGGGCTTCAATATTCAGAAGTAACCCTT
GAAGTGGTTCCCTTGATTCTATCAGATGTCTTGAAGTTACCAATCAAAGAATTGGTGCCTGTGGAAGACAGTGAAGCATA
A

Upstream 100 bases:

>100_bases
TCTTTTTAATTTTCTGAAAAAGTTGCTTTTATAATGGAATCTGTTATAATGGTTTCAGTTATTTTTGTGATAATTTTTAA
AAAATTAGGAGGAGTATAAG

Downstream 100 bases:

>100_bases
AAAAATCATTGAGATGATAAGTCTCAATGATTTTTCTTTTCTTTTTGATGAGTAGCAATATAGGCCAATTTCTGGCTTCT
TGTTTTTCGTTTTTTAAAGA

Product: putative cation (K+) transport protein

Products: K (I) [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 666; Mature: 665

Protein sequence:

>666_residues
MSDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTIQSLVENQGGVNQVSESFILGSISLIIWTLTLITTIKYVLIALKA
DNHHEGGIFSLFTLVRKMSPWLIIPAMIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLF
GIQRFGTGFIGKIFGPVMFIWFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENHRGIFILGSIFLATTGAEALYSD
LGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKHSGIELNPFFASVPSQLRVYLVSLATLAAIIASQALISGSFTLVS
EAMRLKIFPLFRVTYPGANLGQLYIPVINWILFAVTSCTVLAFRTSAHMEAAYGLAITITMLMTTILLKYYLIKKGTRPI
LAHLVMAFFALVEFIFFLASAIKFMHGGYAVVILALAIVFVMFIWHAGTRIVFKYVKSLNLNDYKEQIKQLRDDVCFDLY
QTNVVYLSNRMQDHMIDRSILYSILDKRPKRAQVYWFVNVQVTDEPYTAKYKVDMMGTDYMVRVNLYLGFRMPQTVPRYL
RTIVQDLMESGRLPKQEQEYTITPGRDVGDFRFVLIEERVSNARQLSNFERFIMQTKASIKHVTASPMRWFGLQYSEVTL
EVVPLILSDVLKLPIKELVPVEDSEA

Sequences:

>Translated_666_residues
MSDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTIQSLVENQGGVNQVSESFILGSISLIIWTLTLITTIKYVLIALKA
DNHHEGGIFSLFTLVRKMSPWLIIPAMIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLF
GIQRFGTGFIGKIFGPVMFIWFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENHRGIFILGSIFLATTGAEALYSD
LGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKHSGIELNPFFASVPSQLRVYLVSLATLAAIIASQALISGSFTLVS
EAMRLKIFPLFRVTYPGANLGQLYIPVINWILFAVTSCTVLAFRTSAHMEAAYGLAITITMLMTTILLKYYLIKKGTRPI
LAHLVMAFFALVEFIFFLASAIKFMHGGYAVVILALAIVFVMFIWHAGTRIVFKYVKSLNLNDYKEQIKQLRDDVCFDLY
QTNVVYLSNRMQDHMIDRSILYSILDKRPKRAQVYWFVNVQVTDEPYTAKYKVDMMGTDYMVRVNLYLGFRMPQTVPRYL
RTIVQDLMESGRLPKQEQEYTITPGRDVGDFRFVLIEERVSNARQLSNFERFIMQTKASIKHVTASPMRWFGLQYSEVTL
EVVPLILSDVLKLPIKELVPVEDSEA
>Mature_665_residues
SDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTIQSLVENQGGVNQVSESFILGSISLIIWTLTLITTIKYVLIALKAD
NHHEGGIFSLFTLVRKMSPWLIIPAMIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLFG
IQRFGTGFIGKIFGPVMFIWFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENHRGIFILGSIFLATTGAEALYSDL
GHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKHSGIELNPFFASVPSQLRVYLVSLATLAAIIASQALISGSFTLVSE
AMRLKIFPLFRVTYPGANLGQLYIPVINWILFAVTSCTVLAFRTSAHMEAAYGLAITITMLMTTILLKYYLIKKGTRPIL
AHLVMAFFALVEFIFFLASAIKFMHGGYAVVILALAIVFVMFIWHAGTRIVFKYVKSLNLNDYKEQIKQLRDDVCFDLYQ
TNVVYLSNRMQDHMIDRSILYSILDKRPKRAQVYWFVNVQVTDEPYTAKYKVDMMGTDYMVRVNLYLGFRMPQTVPRYLR
TIVQDLMESGRLPKQEQEYTITPGRDVGDFRFVLIEERVSNARQLSNFERFIMQTKASIKHVTASPMRWFGLQYSEVTLE
VVPLILSDVLKLPIKELVPVEDSEA

Specific function: Transport of potassium into the cell

COG id: COG3158

COG function: function code P; K+ transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAK/KUP transporter (TC 2.A.72) family

Homologues:

Organism=Escherichia coli, GI48994960, Length=525, Percent_Identity=32.9523809523809, Blast_Score=252, Evalue=5e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KUP_STRP1 (Q99Z39)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- EMBL:   CP000017
- RefSeq:   NP_269507.1
- RefSeq:   YP_282515.1
- RefSeq:   YP_282516.1
- ProteinModelPortal:   Q99Z39
- EnsemblBacteria:   EBSTRT00000001697
- EnsemblBacteria:   EBSTRT00000064128
- GeneID:   3571737
- GeneID:   3571738
- GeneID:   901476
- GenomeReviews:   AE004092_GR
- KEGG:   spy:SPy_1414
- GeneTree:   EBGT00050000028227
- HOGENOM:   HBG351154
- OMA:   HLLFSPE
- BioCyc:   SPYO160490:SPY1414-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1152-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1153-MONOMER
- HAMAP:   MF_01522
- InterPro:   IPR003855
- InterPro:   IPR023051

Pfam domain/function: PF02705 K_trans

EC number: NA

Molecular weight: Translated: 74608; Mature: 74477

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x40641e54)-; HASH(0xd9eba44)-; HASH(0xde0348c)-; HASH(0xddc4298)-; HASH(0xe12650c)-; HASH(0xd622298)-; HASH(0xe14a790)-; HASH(0xc9e5198)-; HASH(0xc29df60)-; HASH(0xde26f9c)-; HASH(0xd959ec8)-; HASH(0xd1317f8)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTIQSLVENQGGVNQVSESFILGSISL
CCCCCCCHHHCCCCCCEEEEEEEHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IIWTLTLITTIKYVLIALKADNHHEGGIFSLFTLVRKMSPWLIIPAMIGGATLLSDGALT
HHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCEEEHHHHCCHHHCCCCCCC
PAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLFGIQRFGTGFIGKIFGPVMFI
HHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
WFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENHRGIFILGSIFLATTGAEALYSD
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEEHHHHHHCCHHHHHHH
LGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKHSGIELNPFFASVPSQLRVYLVSLA
HCCCCCCCEEEECHHHHHHHHHHHCCCEEEEEEECCCCEEECCHHHHCHHHHHHHHHHHH
TLAAIIASQALISGSFTLVSEAMRLKIFPLFRVTYPGANLGQLYIPVINWILFAVTSCTV
HHHHHHHHHHHHCCCHHHHHHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
LAFRTSAHMEAAYGLAITITMLMTTILLKYYLIKKGTRPILAHLVMAFFALVEFIFFLAS
HEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AIKFMHGGYAVVILALAIVFVMFIWHAGTRIVFKYVKSLNLNDYKEQIKQLRDDVCFDLY
HHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QTNVVYLSNRMQDHMIDRSILYSILDKRPKRAQVYWFVNVQVTDEPYTAKYKVDMMGTDY
HCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCEEEEEEEEECCCE
MVRVNLYLGFRMPQTVPRYLRTIVQDLMESGRLPKQEQEYTITPGRDVGDFRFVLIEERV
EEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEEEHHHH
SNARQLSNFERFIMQTKASIKHVTASPMRWFGLQYSEVTLEVVPLILSDVLKLPIKELVP
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCC
VEDSEA
CCCCCH
>Mature Secondary Structure 
SDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTIQSLVENQGGVNQVSESFILGSISL
CCCCCCHHHCCCCCCEEEEEEEHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IIWTLTLITTIKYVLIALKADNHHEGGIFSLFTLVRKMSPWLIIPAMIGGATLLSDGALT
HHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCEEEHHHHCCHHHCCCCCCC
PAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLFGIQRFGTGFIGKIFGPVMFI
HHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
WFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENHRGIFILGSIFLATTGAEALYSD
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEEHHHHHHCCHHHHHHH
LGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKHSGIELNPFFASVPSQLRVYLVSLA
HCCCCCCCEEEECHHHHHHHHHHHCCCEEEEEEECCCCEEECCHHHHCHHHHHHHHHHHH
TLAAIIASQALISGSFTLVSEAMRLKIFPLFRVTYPGANLGQLYIPVINWILFAVTSCTV
HHHHHHHHHHHHCCCHHHHHHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
LAFRTSAHMEAAYGLAITITMLMTTILLKYYLIKKGTRPILAHLVMAFFALVEFIFFLAS
HEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AIKFMHGGYAVVILALAIVFVMFIWHAGTRIVFKYVKSLNLNDYKEQIKQLRDDVCFDLY
HHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QTNVVYLSNRMQDHMIDRSILYSILDKRPKRAQVYWFVNVQVTDEPYTAKYKVDMMGTDY
HCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCEEEEEEEEECCCE
MVRVNLYLGFRMPQTVPRYLRTIVQDLMESGRLPKQEQEYTITPGRDVGDFRFVLIEERV
EEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEEEHHHH
SNARQLSNFERFIMQTKASIKHVTASPMRWFGLQYSEVTLEVVPLILSDVLKLPIKELVP
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCC
VEDSEA
CCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: K (I) [Periplasm] [C]

Specific reaction: K (I) [Periplasm] = K (I) [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11296296