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The map label for this gene is nrdE.2 [H]

Identifier: 15675307

GI number: 15675307

Start: 1141909

End: 1144068

Strand: Direct

Name: nrdE.2 [H]

Synonym: SPy_1375

Alternate gene names: 15675307

Gene position: 1141909-1144068 (Clockwise)

Preceding gene: 15675306

Following gene: 15675308

Centisome position: 61.64

GC content: 37.96

Gene sequence:

>2160_bases
ATGAGTCTCAAAGATCTTGGCGATATTTCATATTTTCGCCTAAATAATGAAATTAACCGTCCTGTTAATGGTAAAATTCC
ACTTCATAAAGACAAAGAAGCTTTAAAAGCTTTTTCCGCTGAAAATGTGCTGCCAAACACCATGTCTTTTACTTCCATTA
CGGAAAAAATTGAGTATTTAATCTCAAATGATTACATTGAATCAGCTTTTATTCAGAAATACCGCCCTGAATTTATTACT
GAATTAGATAGCATAATCAAATCAGAAAATTTTCGCTTTAAATCATTTATGGCAGCCTACAAGTTCTACCAGCAATACGC
CTTAAAAACAAATGATGGAGAGCATTATTTAGAAAACCTTGAAGACCGTGTCTTGTTTAATGCTTTGTATTTTGCAGATG
GTCAAGAAGACTTAGCAAAAGATTTAGCCGTTGAAATGATTAACCAACGTTACCAACCGGCTACTCCTTCCTTTTTAAAT
GCTGGTCGAAGCCGTCGTGGTGAATTGGTCTCTTGTTTCTTGATTCAAGTAACTGATGACATGAACTCTATCGGACGTTC
TATCAACTCTGCTTTGCAATTATCCCGTATTGGTGGAGGAGTTGGGATTACCTTGTCTAACCTCCGTGAAGCTGGCGCAC
CAATCAAAGGCTATGCTGGTGCAGCCTCAGGAGTTGTTCCTGTTATGAAATTATTTGAAGATAGTTTTTCTTATTCAAAT
CAACTTGGGCAACGTCAAGGAGCTGGTGTTGTTTACCTAAATGTTTTTCATCCTGATATCATTGCTTTCTTATCTACTAA
AAAAGAAAATGCCGATGAAAAGGTGCGTGTTAAAACCTTGTCACTAGGGATTACCGTTCCTGATAAATTCTACGAATTAG
CTCGTAAAAACGAGGACATGTATCTCTTTAGTCCTTACAATGTTGAAAAAGAATATGGCATTCCCTTTAACTATCTCGAC
ATTACCAATATGTACGATGAGTTAGTGGCGAACCCTAAAATTACTAAGACTAAAATTAAAGCTCGTGATCTTGAAACAGA
GATTTCAAAATTACAACAAGAATCTGGTTACCCTTATATCATCAATATTGATACAGCTAATAAAGCTAATCCTATCGATG
GAAAAATCATCATGAGCAACTTGTGTTCTGAAATTTTACAAGTTCAAACACCTAGCCTTATCAATGATGCGCAAGAGTTT
GTAGAAATGGGAACTGATATTTCATGTAACTTAGGTTCCACTAATATCCTGAACATGATGACCTCACCAGACTTTGGCCG
TTCTATTAAGACCATGACACGTGCCCTAACTTTTGTTACTGATTCATCAAGCATTGAAGCTGTTCCAACCATTAAACATG
GCAATAGCCAAGCTCATACTTTTGGCCTTGGAGCTATGGGACTACATTCTTACCTTGCTCAACATCATATTGAATATGGC
AGTCCAGAATCCATCGAGTTTACTGATATTTACTTTATGCTCCTGAATTATTGGACCTTGGTCGAATCCAATAACATCGC
TCGTGAGCGCCAAACTACCTTTGTTGGCTTTGAGAACTCTAAGTACGCTAATGGTAGTTACTTTGATAAATACGTTACAG
GACACTTTGTTCCAAAATCTGATTTGGTGAAAGATCTGTTCAAAGACCATTTTATTCCGCAAGCTTCAGATTGGGAGGCT
CTTCGCGACGCCGTTCAAAAAGATGGTCTTTATCATCAAAACCGACTAGCAGTTGCTCCAAATGGCTCTATTTCTTATAT
CAATGACTGCTCTGCTTCTATTCACCCAATCACACAACGCATCGAAGAGCGTCAAGAAAAGAAAATTGGTAAAATCTACT
ATCCTGCAAATGGTTTGTCTACGGATACCATTCCTTACTATACATCTGCTTACGATATGGACATGCGCAAAGTTATTGAT
GTCTATGCCGCTGCGACCGAACATGTGGACCAAGGCTTGTCATTAACTCTATTCCTTCGTAGTGAGTTGCCTATGGAGCT
TTATGAGTGGAAAACACAAAGCAAACAAACCACTCGTGATTTATCCATCTTACGAAACTACGCTTTCAATAAAGGCATTA
AATCTATCTACTATATCCGTACCTTTACGGATGATGGGGAAGAAGTGGGCGCAAACCAATGTGAATCTTGTGTCATTTAA

Upstream 100 bases:

>100_bases
TTCACATCTGCTCCTGTTATCGAAGCAGATAACCTCGTATTTTCTGGCTTCCAACCTGCAAAGTTAAAAGAATTGATTTA
ATAAGACAAGGAAAAATAAT

Downstream 100 bases:

>100_bases
TTGGTTAAGAAGTAATGATGCTAAAGCATCTATTACTTCTACGGCAGTCGCTTTAGGCGATCTGCCTAAACCACTCACTA
ACTACGTAAGGAAAGTATTA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 719; Mature: 718

Protein sequence:

>719_residues
MSLKDLGDISYFRLNNEINRPVNGKIPLHKDKEALKAFSAENVLPNTMSFTSITEKIEYLISNDYIESAFIQKYRPEFIT
ELDSIIKSENFRFKSFMAAYKFYQQYALKTNDGEHYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLN
AGRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYAGAASGVVPVMKLFEDSFSYSN
QLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLD
ITNMYDELVANPKITKTKIKARDLETEISKLQQESGYPYIINIDTANKANPIDGKIIMSNLCSEILQVQTPSLINDAQEF
VEMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSSIEAVPTIKHGNSQAHTFGLGAMGLHSYLAQHHIEYG
SPESIEFTDIYFMLLNYWTLVESNNIARERQTTFVGFENSKYANGSYFDKYVTGHFVPKSDLVKDLFKDHFIPQASDWEA
LRDAVQKDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTIPYYTSAYDMDMRKVID
VYAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLSILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI

Sequences:

>Translated_719_residues
MSLKDLGDISYFRLNNEINRPVNGKIPLHKDKEALKAFSAENVLPNTMSFTSITEKIEYLISNDYIESAFIQKYRPEFIT
ELDSIIKSENFRFKSFMAAYKFYQQYALKTNDGEHYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLN
AGRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYAGAASGVVPVMKLFEDSFSYSN
QLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLD
ITNMYDELVANPKITKTKIKARDLETEISKLQQESGYPYIINIDTANKANPIDGKIIMSNLCSEILQVQTPSLINDAQEF
VEMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSSIEAVPTIKHGNSQAHTFGLGAMGLHSYLAQHHIEYG
SPESIEFTDIYFMLLNYWTLVESNNIARERQTTFVGFENSKYANGSYFDKYVTGHFVPKSDLVKDLFKDHFIPQASDWEA
LRDAVQKDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTIPYYTSAYDMDMRKVID
VYAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLSILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI
>Mature_718_residues
SLKDLGDISYFRLNNEINRPVNGKIPLHKDKEALKAFSAENVLPNTMSFTSITEKIEYLISNDYIESAFIQKYRPEFITE
LDSIIKSENFRFKSFMAAYKFYQQYALKTNDGEHYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLNA
GRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYAGAASGVVPVMKLFEDSFSYSNQ
LGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLDI
TNMYDELVANPKITKTKIKARDLETEISKLQQESGYPYIINIDTANKANPIDGKIIMSNLCSEILQVQTPSLINDAQEFV
EMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSSIEAVPTIKHGNSQAHTFGLGAMGLHSYLAQHHIEYGS
PESIEFTDIYFMLLNYWTLVESNNIARERQTTFVGFENSKYANGSYFDKYVTGHFVPKSDLVKDLFKDHFIPQASDWEAL
RDAVQKDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTIPYYTSAYDMDMRKVIDV
YAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLSILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=616, Percent_Identity=25.1623376623377, Blast_Score=150, Evalue=4e-36,
Organism=Escherichia coli, GI1789030, Length=698, Percent_Identity=49.5702005730659, Blast_Score=712, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=599, Percent_Identity=25.3756260434057, Blast_Score=161, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17555242, Length=622, Percent_Identity=25.5627009646302, Blast_Score=161, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6322123, Length=595, Percent_Identity=24.7058823529412, Blast_Score=148, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320914, Length=602, Percent_Identity=23.421926910299, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI17352481, Length=479, Percent_Identity=26.9311064718163, Blast_Score=147, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 81353; Mature: 81222

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKDLGDISYFRLNNEINRPVNGKIPLHKDKEALKAFSAENVLPNTMSFTSITEKIEYL
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ISNDYIESAFIQKYRPEFITELDSIIKSENFRFKSFMAAYKFYQQYALKTNDGEHYLENL
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
EDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLNAGRSRRGELVSCFLIQVTDD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEEEEEECHH
MNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYAGAASGVVPVMKLFEDSFSYSN
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGITVPDKFYELARKNEDM
HHHHCCCCCEEEEEECCHHHHHHHHCCCCCCCCEEEEEEEEEEEECCHHHHHHHHCCCCE
YLFSPYNVEKEYGIPFNYLDITNMYDELVANPKITKTKIKARDLETEISKLQQESGYPYI
EEECCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
INIDTANKANPIDGKIIMSNLCSEILQVQTPSLINDAQEFVEMGTDISCNLGSTNILNMM
EEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHCC
TSPDFGRSIKTMTRALTFVTDSSSIEAVPTIKHGNSQAHTFGLGAMGLHSYLAQHHIEYG
CCCCCCHHHHHHHHHHHEEECCCCCCCCEEEECCCCCCEEEECHHHHHHHHHHHHHCCCC
SPESIEFTDIYFMLLNYWTLVESNNIARERQTTFVGFENSKYANGSYFDKYVTGHFVPKS
CCCCEEHHHHHHHHHHHHHHCCCCCCCHHHCCEEEECCCCCCCCCCHHHHHHCCCCCCHH
DLVKDLFKDHFIPQASDWEALRDAVQKDGLYHQNRLAVAPNGSISYINDCSASIHPITQR
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEECCEEEECCCCCEEEECCCCCCHHHHHHH
IEERQEKKIGKIYYPANGLSTDTIPYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLR
HHHHHHHCCCEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEE
SELPMELYEWKTQSKQTTRDLSILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHCCHHHHHCCC
>Mature Secondary Structure 
SLKDLGDISYFRLNNEINRPVNGKIPLHKDKEALKAFSAENVLPNTMSFTSITEKIEYL
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ISNDYIESAFIQKYRPEFITELDSIIKSENFRFKSFMAAYKFYQQYALKTNDGEHYLENL
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
EDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLNAGRSRRGELVSCFLIQVTDD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEEEEEECHH
MNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYAGAASGVVPVMKLFEDSFSYSN
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGITVPDKFYELARKNEDM
HHHHCCCCCEEEEEECCHHHHHHHHCCCCCCCCEEEEEEEEEEEECCHHHHHHHHCCCCE
YLFSPYNVEKEYGIPFNYLDITNMYDELVANPKITKTKIKARDLETEISKLQQESGYPYI
EEECCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
INIDTANKANPIDGKIIMSNLCSEILQVQTPSLINDAQEFVEMGTDISCNLGSTNILNMM
EEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHCC
TSPDFGRSIKTMTRALTFVTDSSSIEAVPTIKHGNSQAHTFGLGAMGLHSYLAQHHIEYG
CCCCCCHHHHHHHHHHHEEECCCCCCCCEEEECCCCCCEEEECHHHHHHHHHHHHHCCCC
SPESIEFTDIYFMLLNYWTLVESNNIARERQTTFVGFENSKYANGSYFDKYVTGHFVPKS
CCCCEEHHHHHHHHHHHHHHCCCCCCCHHHCCEEEECCCCCCCCCCHHHHHHCCCCCCHH
DLVKDLFKDHFIPQASDWEALRDAVQKDGLYHQNRLAVAPNGSISYINDCSASIHPITQR
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEECCEEEECCCCCEEEECCCCCCHHHHHHH
IEERQEKKIGKIYYPANGLSTDTIPYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLR
HHHHHHHCCCEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEE
SELPMELYEWKTQSKQTTRDLSILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]