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The map label for this gene is pstI [H]

Identifier: 15675304

GI number: 15675304

Start: 1139277

End: 1141010

Strand: Reverse

Name: pstI [H]

Synonym: SPy_1372

Alternate gene names: 15675304

Gene position: 1141010-1139277 (Counterclockwise)

Preceding gene: 15675305

Following gene: 15675303

Centisome position: 61.59

GC content: 43.08

Gene sequence:

>1734_bases
ATGACAGAAATGCTTAAAGGAATTGCAGCCTCAGACGGCGTTGCTGTTGCTAAAGCATATCTACTAGTTCAGCCGGATTT
GTCATTTGAGACTGTTACAGTCGCAGATACAAATGCAGAAGAAGCTCGCCTTGATGTTGCACTCCAAGCTGCACAAGACG
AGCTTTCTGTTATCCGTGAAAATGCAGTAGAAAGCTTAGGTGAAGAAGCAGCAGCCGTTTTTGATGCCCATTTGATGGTT
CTTGCTGATCCAGAAATGATCAGCCAGGTTAAAGAAACGATTCGCGCAAAACAAACGAATGCAGAAACAGGTCTTAAAGA
AGTGACTGACATGTTCATCACCATCTTTGAAGGCATGGAAGATAACCCATACATGCAAGAACGTGCAGCGGACATCCGCG
ACGTTGCAAAACGTGTGTTGGCTCACCTTTTAGGTGTAAAACTTCCAAATCCAGCTACAATCAATGAAGAATCAATCGTT
ATCGCACACGATTTGACACCTTCAGATACTGCTCAACTTAACAAACAATTTGTAAAAGCATTTGTTACAAATATCGGTGG
TCGTACAAGTCACTCAGCTATCATGGCACGTACACTTGAGATCGCTGCGGTACTTGGAACAAATGATATTACAAAACGTG
TTAAAGATGGTGATGTGATTGCCGTTAATGGTATCACTGGTGAAGTGATTATCGATCCAAGCGAAGATCAAGTACTTGCT
TTTAAAGAAGCTGGTGCGGCTTATGCCAAACAAAAAGCAGAGTGGTCTCTCCTTAAAGATGCGCACACTGAAACAGCTGA
TGGCAAACACTTTGAATTGGCTGCTAATATCGGTACGCCTAAAGACGTTGAAGGTGTTAATGACAATGGTGCTGAAGCTG
TTGGCCTTTACCGTACTGAGTTCTTGTACATGGATTCTCAAGACTTCCCAACTGAAGACGAACAATACGAAGCTTACAAG
GCAGTGCTTGAAGGCATGAATGGCAAACCTGTTGTGGTTCGTACGATGGATATTGGTGGCGACAAGGAACTTCCTTACTT
TGACCTTCCAAAAGAAATGAATCCATTCCTTGGTTTCCGTGCTCTTCGTATTTCCATCTCTGAAACTGGGGATGCCATGT
TCCGCACACAAATGCGTGCGCTTCTTCGTGCCTCTGTTCACGGACAACTTCGTATTATGTTCCCAATGGTTGCGCTTCTT
AAAGAATTCCGTGCTGCAAAAGCAGTCTTTGACGAAGAAAAAGCAAACTTGCTTGCAGAAGGCGTTGCGGTTGCTGATGA
CATCCAAGTTGGTATCATGATTGAGATTCCTGCAGCTGCTATGCTTGCAGACCAATTTGCTAAGGAAGTTGATTTCTTCT
CAATTGGAACAAACGACCTTATCCAATACACTATGGCAGCAGACCGTATGAACGAACAAGTATCATACCTTTACCAACCA
TACAACCCATCAATATTACGTTTGATCAACAATGTGATCAAAGCAGCGCACGCTGAAGGTAAATGGGCAGGTATGTGTGG
TGAGATGGCAGGTGACCAACAAGCTGTTCCACTTCTTGTCGGAATGGGCTTGGATGAGTTTTCTATGTCAGCAACTTCAG
TACTTCGTACGCGTAGTTTAATGAAGAAACTTGACTCTGCTAAGATGGAAGAATATGCAAATCGTGCGCTTACAGAATGT
TCAACAGCAGAAGAAGTTCTTGAACTTTCTAAAGAATACGTTTCTGAAGATTAA

Upstream 100 bases:

>100_bases
CAAGGTGCAGATGTTACTATCTCAGCTGAAGGTGCAGATGCTGAAGATGCCATTGCAGCAATTGAAGAAACAATGACTAA
AGAGGGATTGGCATAAGACT

Downstream 100 bases:

>100_bases
AGGGTTAAAGAGTTGGTTTAAACCAGCTCTTTTTTGCTAGATAAAAATACAATTACTTTTTGGTGTGAAATCATTTGAGT
TATCAAATAATTATCAGAAT

Product: putative phosphoenolpyruvate:sugar phosphotransferase system enzyme I

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARLDVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMV
LADPEMISQVKETIRAKQTNAETGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGDVIAVNGITGEVIIDPSEDQVLA
FKEAGAAYAKQKAEWSLLKDAHTETADGKHFELAANIGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYK
AVLEGMNGKPVVVRTMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRIMFPMVALL
KEFRAAKAVFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQP
YNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTEC
STAEEVLELSKEYVSED

Sequences:

>Translated_577_residues
MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARLDVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMV
LADPEMISQVKETIRAKQTNAETGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGDVIAVNGITGEVIIDPSEDQVLA
FKEAGAAYAKQKAEWSLLKDAHTETADGKHFELAANIGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYK
AVLEGMNGKPVVVRTMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRIMFPMVALL
KEFRAAKAVFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQP
YNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTEC
STAEEVLELSKEYVSED
>Mature_576_residues
TEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARLDVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMVL
ADPEMISQVKETIRAKQTNAETGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIVI
AHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGDVIAVNGITGEVIIDPSEDQVLAF
KEAGAAYAKQKAEWSLLKDAHTETADGKHFELAANIGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKA
VLEGMNGKPVVVRTMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRIMFPMVALLK
EFRAAKAVFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQPY
NPSILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTECS
TAEEVLELSKEYVSED

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=575, Percent_Identity=45.9130434782609, Blast_Score=513, Evalue=1e-147,
Organism=Escherichia coli, GI48994992, Length=504, Percent_Identity=36.9047619047619, Blast_Score=338, Evalue=6e-94,
Organism=Escherichia coli, GI1788726, Length=590, Percent_Identity=36.271186440678, Blast_Score=328, Evalue=7e-91,
Organism=Escherichia coli, GI1789193, Length=573, Percent_Identity=32.6352530541012, Blast_Score=278, Evalue=9e-76,
Organism=Escherichia coli, GI1787994, Length=417, Percent_Identity=29.9760191846523, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI226510935, Length=176, Percent_Identity=30.1136363636364, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63137; Mature: 63005

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARLDVALQAAQDELSVIRE
CCHHHHHHCCCCCHHEEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
NAVESLGEEAAAVFDAHLMVLADPEMISQVKETIRAKQTNAETGLKEVTDMFITIFEGME
HHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
DNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIVIAHDLTPSDTAQLNKQFVKA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHH
FVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGDVIAVNGITGEVIIDPSEDQVLA
HHHHCCCCCCHHHHHHHHHHHHHEECCCHHHHHCCCCCEEEEECCCEEEEECCCCCCEEE
FKEAGAAYAKQKAEWSLLKDAHTETADGKHFELAANIGTPKDVEGVNDNGAEAVGLYRTE
EHHHCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEE
FLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVRTMDIGGDKELPYFDLPKEMNPFLGFR
EEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHCEE
ALRISISETGDAMFRTQMRALLRASVHGQLRIMFPMVALLKEFRAAKAVFDEEKANLLAE
EEEEEECCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVAVADDIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQP
CCEEECCCEEEEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCC
YNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSL
CCCHHHHHHHHHHHHHCCCCCHHHCHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
MKKLDSAKMEEYANRALTECSTAEEVLELSKEYVSED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARLDVALQAAQDELSVIRE
CHHHHHHCCCCCHHEEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
NAVESLGEEAAAVFDAHLMVLADPEMISQVKETIRAKQTNAETGLKEVTDMFITIFEGME
HHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
DNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIVIAHDLTPSDTAQLNKQFVKA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHH
FVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGDVIAVNGITGEVIIDPSEDQVLA
HHHHCCCCCCHHHHHHHHHHHHHEECCCHHHHHCCCCCEEEEECCCEEEEECCCCCCEEE
FKEAGAAYAKQKAEWSLLKDAHTETADGKHFELAANIGTPKDVEGVNDNGAEAVGLYRTE
EHHHCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEE
FLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVRTMDIGGDKELPYFDLPKEMNPFLGFR
EEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHCEE
ALRISISETGDAMFRTQMRALLRASVHGQLRIMFPMVALLKEFRAAKAVFDEEKANLLAE
EEEEEECCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVAVADDIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQP
CCEEECCCEEEEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCC
YNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSL
CCCHHHHHHHHHHHHHCCCCCHHHCHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
MKKLDSAKMEEYANRALTECSTAEEVLELSKEYVSED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA