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The map label for this gene is aroA

Identifier: 15675285

GI number: 15675285

Start: 1117577

End: 1118869

Strand: Reverse

Name: aroA

Synonym: SPy_1352

Alternate gene names: 15675285

Gene position: 1118869-1117577 (Counterclockwise)

Preceding gene: 15675286

Following gene: 15675284

Centisome position: 60.4

GC content: 41.14

Gene sequence:

>1293_bases
ATGAAACGGATGAAATTGCGAACAAATGCAGGACCATTACAGGGAACGATTCAAGTTCCAGGAGACAAGTCCATTAGTCA
CCGGGCAGTCATATTAGGAGCCGTTGCTAAAGGGGAAACACGAGTAAAAGGACTCTTAAAAGGAGAAGATGTGCTTTCCA
CGATTCAAGCCTTTAGGAATTTGGGGGTCCGTATCGAAGAAAAAGATGACCAGCTTGTGATAGAAGGTCAAGGTTTTCAA
GGATTGAACGCTCCTTGTCAGACACTTAATATGGGAAATTCTGGAACCTCTATGCGCCTCATTGCTGGCTTATTGGCAGG
TCAACCTTTTAGTGTCAAAATGATTGGCGATGAAAGCTTATCCAAACGTCCTATGGATCGGATTGTTTATCCGTTAAAGC
AAATGGGGGTTGAGATATCTGGAGAAACAGATAGGCAATTTCCTCCACTACAATTGCAAGGTAATCGTAATTTACAACCA
ATCACATATACCTTGCCTATCTCTTCTGCTCAGGTTAAGTCAGCTATTTTATTAGCAGCTTTGCAAGCAAAAGGAACAAC
ACAGGTTGTTGAAAAAGAGATTACTCGAAACCATACAGAAGAAATGATTCAACAATTTGGTGGACGATTGATAGTTGATG
GTAAACGCATTACTTTGGTGGGACCCCAGCAGCTCACTGCTCAAGAGATAACTGTCCCCGGAGATATTTCTAGTGCAGCT
TTTTGGCTTGTCGCCGGCTTAATTATTCCTGGTTCGGAGCTCCTTTTAAAGAATGTTGGAGTCAATCCGACACGAACAGG
GATTCTTGAAGTGGTTGAAAAAATGGGAGCACAGATTGTCTATGAAGATATGAATAAGAAAGAGCAAGTAACTAGTATTC
GAGTGGTATATTCTAACATGAAAGGAACTATCATATCAGGAGGTCTTATCCCTCGATTGATTGATGAACTGCCCATCATT
GCACTTCTTGCAACCCAAGCACAAGGAACAACCTGTATTAAAGATGCTCAAGAATTACGAGTCAAAGAAACAGATCGTAT
TCAAGTAGTAACTGATATTCTTAACAGTATGGGAGCTAATATCAAAGCGACAGCAGATGGCATGATTATCAAAGGCCCTA
CGGTACTATATGGGGCTAACACAAGTACCTATGGAGATCATCGTATTGGTATGATGACAGCAATTGCAGCCTTGCTTGTT
AAACAAGGACAGGTCCATTTGGATAAAGAAGAAGCCATCATGACGTCTTATCCAACGTTTTTCAAAGATTTAGAAAGGCT
GTGTCATGACTAA

Upstream 100 bases:

>100_bases
ACAAAAAAGATAATAAGTCGTGGAGTGAGAAACTATTTTCTTGCTTCATTTTTTATGAGGATGAATAGAAAATCATTGAT
CTTAGCAAATAGAAAGGCAG

Downstream 100 bases:

>100_bases
AGTGTTATTGGGATTTATGGGAGTGGGGAAAACAACGGTTTCGAAACACCTAAGTATGCACTGTAAAGATATGGATGCTA
TTATTGAAGCTAAAATCGGT

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKRMKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLKGEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQ
GLNAPCQTLNMGNSGTSMRLIAGLLAGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQP
ITYTLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVGPQQLTAQEITVPGDISSAA
FWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQIVYEDMNKKEQVTSIRVVYSNMKGTIISGGLIPRLIDELPII
ALLATQAQGTTCIKDAQELRVKETDRIQVVTDILNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAIAALLV
KQGQVHLDKEEAIMTSYPTFFKDLERLCHD

Sequences:

>Translated_430_residues
MKRMKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLKGEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQ
GLNAPCQTLNMGNSGTSMRLIAGLLAGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQP
ITYTLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVGPQQLTAQEITVPGDISSAA
FWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQIVYEDMNKKEQVTSIRVVYSNMKGTIISGGLIPRLIDELPII
ALLATQAQGTTCIKDAQELRVKETDRIQVVTDILNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAIAALLV
KQGQVHLDKEEAIMTSYPTFFKDLERLCHD
>Mature_430_residues
MKRMKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLKGEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQ
GLNAPCQTLNMGNSGTSMRLIAGLLAGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQP
ITYTLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVGPQQLTAQEITVPGDISSAA
FWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQIVYEDMNKKEQVTSIRVVYSNMKGTIISGGLIPRLIDELPII
ALLATQAQGTTCIKDAQELRVKETDRIQVVTDILNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAIAALLV
KQGQVHLDKEEAIMTSYPTFFKDLERLCHD

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=431, Percent_Identity=29.2343387470998, Blast_Score=128, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6320332, Length=433, Percent_Identity=24.4803695150115, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_STRP1 (Q99Z83)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269459.1
- RefSeq:   YP_282464.1
- ProteinModelPortal:   Q99Z83
- SMR:   Q99Z83
- EnsemblBacteria:   EBSTRT00000000861
- EnsemblBacteria:   EBSTRT00000028278
- GeneID:   3571803
- GeneID:   901427
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1352
- KEGG:   spz:M5005_Spy_1101
- GeneTree:   EBGT00050000028044
- HOGENOM:   HBG646626
- OMA:   LSRNHTE
- ProtClustDB:   PRK02427
- BioCyc:   SPYO160490:SPY1352-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1101-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 46693; Mature: 46693

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRMKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLKGEDVLSTIQAFRN
CCCEEECCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCHHHHHHHCHHHHHHHHHHHHH
LGVRIEEKDDQLVIEGQGFQGLNAPCQTLNMGNSGTSMRLIAGLLAGQPFSVKMIGDESL
CCCEEECCCCEEEEECCCCCCCCCCHHHEECCCCCCHHHHHHHHHCCCCEEEEEECCCCC
SKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQPITYTLPISSAQVKSAILLAA
CCCCHHHHHHHHHHCCEEECCCCCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHH
LQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVGPQQLTAQEITVPGDISSAA
HHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEECCCCCCCCEEECCCCCHHHH
FWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQIVYEDMNKKEQVTSIRVVYSNM
HHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEHHCCCHHHHHEEEEEEECC
KGTIISGGLIPRLIDELPIIALLATQAQGTTCIKDAQELRVKETDRIQVVTDILNSMGAN
CCEEEECCCHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
IKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAIAALLVKQGQVHLDKEEAIMTSYPTF
EEEECCCEEEECCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEECHHHHHHHCCCHH
FKDLERLCHD
HHHHHHHHCC
>Mature Secondary Structure
MKRMKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLKGEDVLSTIQAFRN
CCCEEECCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCHHHHHHHCHHHHHHHHHHHHH
LGVRIEEKDDQLVIEGQGFQGLNAPCQTLNMGNSGTSMRLIAGLLAGQPFSVKMIGDESL
CCCEEECCCCEEEEECCCCCCCCCCHHHEECCCCCCHHHHHHHHHCCCCEEEEEECCCCC
SKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQPITYTLPISSAQVKSAILLAA
CCCCHHHHHHHHHHCCEEECCCCCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHH
LQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVGPQQLTAQEITVPGDISSAA
HHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEECCCCCCCCEEECCCCCHHHH
FWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQIVYEDMNKKEQVTSIRVVYSNM
HHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEHHCCCHHHHHEEEEEEECC
KGTIISGGLIPRLIDELPIIALLATQAQGTTCIKDAQELRVKETDRIQVVTDILNSMGAN
CCEEEECCCHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
IKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAIAALLVKQGQVHLDKEEAIMTSYPTF
EEEECCCEEEECCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEECHHHHHHHCCCHH
FKDLERLCHD
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296