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The map label for this gene is bglA.2 [H]

Identifier: 15675270

GI number: 15675270

Start: 1102494

End: 1103894

Strand: Reverse

Name: bglA.2 [H]

Synonym: SPy_1328

Alternate gene names: 15675270

Gene position: 1103894-1102494 (Counterclockwise)

Preceding gene: 15675272

Following gene: 15675269

Centisome position: 59.59

GC content: 40.76

Gene sequence:

>1401_bases
ATGAAACAAAGAAAACACCGTTATCAATTTCCAGATGGCTTTTTATGGGGAAGTTCCACATCTGGGCCTCAAAGCGAAGG
AACGGTTCCTGGAGATGGCAAAGGACCTAGCAACTGGGATTACTGGTTTAGTATTGAGTCAGCTAAGTTTCATCACCAAA
TTGGTCCAGAAAAAACCTCAACCTTTTATGAAAACTATAAGGGTGACATTGCCCTTTTGAAAGAAACAGGCCACACGATT
TTTCGGACCTCAATCCAGTGGTCTCGATTGATTCCAGAAGGAGTCGGGGAGGTTAATCCAAAGGCGGTGACATTTTACCG
AGAAGTATTTCAAGACATTATTGCTCAAGGTATTAAATTGATTGTTAACCTCTATCATTTTGACCTCCCCTATGCCTTGC
AAGAAAAAGGTGGCTGGGAAAACAAAGCTACTGTCTGGGCTTATGAAACCTACGCAAAAACCTGTTTTGAGCTTTTTGGT
GATTTAGTAAACACTTGGATTACCTTTAATGAACCGATTGTTCCAGTCGAATGTGGCTATTTAGGTTATTATCACTACCC
TTGTAAAGTAGATGCCAAGGCAGCAGTTCAAGTGGCTTACAACACACAACTGGCCAGTTCACTGGCGGTCAAAGCTTGTC
ATAAGCTTCATCCCGACCACAAGATTAGTATTGTGCTTAACATGACACCTGCTTATCCTCGGAGTAATGCTCCTGAAGAT
GTCAAGGCTGCTAGAATCGCAGAGCTTTTCCAAACCAAATCCTTCCTAGATCCATCAGTTTTGGGTGTTTACCCAGCAGA
GTTAGTTTCCATTTTAGAGGAAGCTGATTTATTACCTCAATATAGTGCTGATGAATTAGAGATTATCAAAAACAATACTG
TTGATTTTTTGGGAGTTAATTATTATCAGCCTTTGCGGGTTCAGGCACCTAGTAAGTCACAGCAAGAGGGGGACCTCCTT
ATCTTAGATATTTATTTTGAACCTTATGATATGCCTGGTAAAAAAGTCAATCCTCACCGGGGTTGGGAAATTTATGAACC
AGGACTTTATGATATTGCCCTTAATTTAAAAGAACACTATGGTAACATCGAATGGCTAGTCACGGAAAATGGTATGGGTG
TCGAAGGTGAAGAAGCTTTTTTAGCAGATGGCCAGATACAAGATGACTACCGCATCACCTTTATCGAGGATCATCTTATC
CAGCTTCATAAGGCTTTGGGAGAAGGAGCCAACTGCAAAGGCTACCTCTTGTGGACTTTTATTGATTGCTGGTCTTGGTT
AAATGCTTATAAAAACCGATATGGTTTGGTAGCTTTGGATTTAGAGAGTCAAAAGCGAACCATAAAAAAATCAGGTTACT
GGTTTAAGGCGTTGAGCGAGTCAAATGGTTTTGATAAATAG

Upstream 100 bases:

>100_bases
TCCGTGATGATAAGGAAAAAACAGATTTTTTAAAGCGTTTACATCACCTGTTTTTCTTCTTATAATAAAAAGAGAAGTGA
GCAAAGAAAGGACAAAGGAT

Downstream 100 bases:

>100_bases
GAGGCAATATGGTTGATTTAGGATTTTCTCTATACCCTGAGCGGTATGATGTTACCAAGAGTAAAGCTTATATTGACTTA
TGTCATAGCTATGGAGCCAA

Product: putative beta-glucosidase

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFSIESAKFHHQIGPEKTSTFYENYKGDIALLKETGHTI
FRTSIQWSRLIPEGVGEVNPKAVTFYREVFQDIIAQGIKLIVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFG
DLVNTWITFNEPIVPVECGYLGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHPDHKISIVLNMTPAYPRSNAPED
VKAARIAELFQTKSFLDPSVLGVYPAELVSILEEADLLPQYSADELEIIKNNTVDFLGVNYYQPLRVQAPSKSQQEGDLL
ILDIYFEPYDMPGKKVNPHRGWEIYEPGLYDIALNLKEHYGNIEWLVTENGMGVEGEEAFLADGQIQDDYRITFIEDHLI
QLHKALGEGANCKGYLLWTFIDCWSWLNAYKNRYGLVALDLESQKRTIKKSGYWFKALSESNGFDK

Sequences:

>Translated_466_residues
MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFSIESAKFHHQIGPEKTSTFYENYKGDIALLKETGHTI
FRTSIQWSRLIPEGVGEVNPKAVTFYREVFQDIIAQGIKLIVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFG
DLVNTWITFNEPIVPVECGYLGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHPDHKISIVLNMTPAYPRSNAPED
VKAARIAELFQTKSFLDPSVLGVYPAELVSILEEADLLPQYSADELEIIKNNTVDFLGVNYYQPLRVQAPSKSQQEGDLL
ILDIYFEPYDMPGKKVNPHRGWEIYEPGLYDIALNLKEHYGNIEWLVTENGMGVEGEEAFLADGQIQDDYRITFIEDHLI
QLHKALGEGANCKGYLLWTFIDCWSWLNAYKNRYGLVALDLESQKRTIKKSGYWFKALSESNGFDK
>Mature_466_residues
MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFSIESAKFHHQIGPEKTSTFYENYKGDIALLKETGHTI
FRTSIQWSRLIPEGVGEVNPKAVTFYREVFQDIIAQGIKLIVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFG
DLVNTWITFNEPIVPVECGYLGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHPDHKISIVLNMTPAYPRSNAPED
VKAARIAELFQTKSFLDPSVLGVYPAELVSILEEADLLPQYSADELEIIKNNTVDFLGVNYYQPLRVQAPSKSQQEGDLL
ILDIYFEPYDMPGKKVNPHRGWEIYEPGLYDIALNLKEHYGNIEWLVTENGMGVEGEEAFLADGQIQDDYRITFIEDHLI
QLHKALGEGANCKGYLLWTFIDCWSWLNAYKNRYGLVALDLESQKRTIKKSGYWFKALSESNGFDK

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI13273313, Length=461, Percent_Identity=31.4533622559653, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI110681710, Length=485, Percent_Identity=31.1340206185567, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI32481206, Length=480, Percent_Identity=29.1666666666667, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI28376633, Length=471, Percent_Identity=29.0870488322718, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI24497614, Length=492, Percent_Identity=27.0325203252033, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI1789070, Length=481, Percent_Identity=33.0561330561331, Blast_Score=224, Evalue=7e-60,
Organism=Escherichia coli, GI2367270, Length=475, Percent_Identity=33.4736842105263, Blast_Score=211, Evalue=9e-56,
Organism=Escherichia coli, GI2367174, Length=486, Percent_Identity=30.2469135802469, Blast_Score=197, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI17552856, Length=477, Percent_Identity=31.8658280922432, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17539390, Length=469, Percent_Identity=31.1300639658849, Blast_Score=205, Evalue=5e-53,
Organism=Drosophila melanogaster, GI21356577, Length=485, Percent_Identity=27.4226804123711, Blast_Score=174, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 53146; Mature: 53146

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFSIESAKFHHQIGPEKTS
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHCCCCHHH
TFYENYKGDIALLKETGHTIFRTSIQWSRLIPEGVGEVNPKAVTFYREVFQDIIAQGIKL
HHHHCCCCCEEEEECCCCEEEEECCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFGDLVNTWITFNEPIVPVECGY
HHHHHHCCCCHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHEECCCCEEEEECCE
LGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHPDHKISIVLNMTPAYPRSNAPED
EEEEECCCEECCCEEEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCH
VKAARIAELFQTKSFLDPSVLGVYPAELVSILEEADLLPQYSADELEIIKNNTVDFLGVN
HHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECC
YYQPLRVQAPSKSQQEGDLLILDIYFEPYDMPGKKVNPHRGWEIYEPGLYDIALNLKEHY
CCCCEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEECCCEEEEEEEEHHHC
GNIEWLVTENGMGVEGEEAFLADGQIQDDYRITFIEDHLIQLHKALGEGANCKGYLLWTF
CCEEEEEECCCCCCCCCCEEEECCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEHHH
IDCWSWLNAYKNRYGLVALDLESQKRTIKKSGYWFKALSESNGFDK
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCEEEEECCCCCCCC
>Mature Secondary Structure
MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFSIESAKFHHQIGPEKTS
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHCCCCHHH
TFYENYKGDIALLKETGHTIFRTSIQWSRLIPEGVGEVNPKAVTFYREVFQDIIAQGIKL
HHHHCCCCCEEEEECCCCEEEEECCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFGDLVNTWITFNEPIVPVECGY
HHHHHHCCCCHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHEECCCCEEEEECCE
LGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHPDHKISIVLNMTPAYPRSNAPED
EEEEECCCEECCCEEEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCH
VKAARIAELFQTKSFLDPSVLGVYPAELVSILEEADLLPQYSADELEIIKNNTVDFLGVN
HHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECC
YYQPLRVQAPSKSQQEGDLLILDIYFEPYDMPGKKVNPHRGWEIYEPGLYDIALNLKEHY
CCCCEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEECCCEEEEEEEEHHHC
GNIEWLVTENGMGVEGEEAFLADGQIQDDYRITFIEDHLIQLHKALGEGANCKGYLLWTF
CCEEEEEECCCCCCCCCCEEEECCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEHHH
IDCWSWLNAYKNRYGLVALDLESQKRTIKKSGYWFKALSESNGFDK
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]