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The map label for this gene is tcyA [H]

Identifier: 15675229

GI number: 15675229

Start: 1050845

End: 1051681

Strand: Reverse

Name: tcyA [H]

Synonym: SPy_1274

Alternate gene names: 15675229

Gene position: 1051681-1050845 (Counterclockwise)

Preceding gene: 15675230

Following gene: 15675228

Centisome position: 56.77

GC content: 33.09

Gene sequence:

>837_bases
ATGATTATAAAAAAAAGAACCGTAGCAATTTTAGCCATAGCTAGTAGCTTTTTCTTGGTAGCTTGTCAAGCTACTAAAAG
TCTTAAATCAGGAGATGCTTGGGGAGTTTACCAAAAGCAAAAAAGTATTACAGTTGGTTTTGACAATACGTTTGTTCCTA
TGGGCTATAAGGATGAAAGCGGCAGATGCAAAGGTTTTGATATTGATTTGGCTAAAGAAGTTTTTCACCAATATGGACTC
AAGGTTAACTTTCAAGCTATTAATTGGGACATGAAAGAAGCAGAACTAAACAATGGTAAAATTGATGTAATCTGGAATGG
TTATTCAATAACTAAGGAGCGTCAGGATAAGGTTGCCTTTACTGATTCTTACATGAGAAATGAACAAATTATTGTTGTCA
AAAAAAGATCTGATATTAAAACAATATCAGATATGAAACATAAAGTGTTAGGAGCACAATCAGCTTCATCAGGTTATGAC
TCCTTGTTAAGAACTCCTAAACTGCTGAAAGATTTTATTAAAAATAAAGACGCTAATCAATATGAAACCTTTACACAAGC
TTTTATTGATTTAAAATCAGATCGTATCGATGGAATATTGATTGACAAAGTATATGCCAATTACTATTTAGCAAAAGAAG
GGCAATTAGAGAATTATCGGATGATCCCAACGACCTTTGAAAATGAAGCATTTTCGGTTGGACTTAGAAAAGAAGACAAA
ACGTTGCAAGCAAAAATTAATCGTGCTTTCAGGGTGCTTTATCAAAATGGCAAATTTCAAGCTATTTCTGAGAAATGGTT
TGGAGATGATGTTGCCACTGCCAATATTAAATCTTAA

Upstream 100 bases:

>100_bases
GGGAATAACTCAAATTGTAGTCACTCACGATCTTGTTTTTGCCGAAGCCATCTCAGATCGTATTATTAGGGTTAATCCTA
AATAGAAGAGGACCCAAAAG

Downstream 100 bases:

>100_bases
AAAGGACACTTGTCCTTTTTTTGTTTAACAGGTGAGATCTAGCAGACAAAGTCAGCTAGACGATTTAATAAAAATCTTAG
TTTAAGGTTTTTTATTAAAC

Product: putative amino acid ABC transporter periplasmic amino acid-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MIIKKRTVAILAIASSFFLVACQATKSLKSGDAWGVYQKQKSITVGFDNTFVPMGYKDESGRCKGFDIDLAKEVFHQYGL
KVNFQAINWDMKEAELNNGKIDVIWNGYSITKERQDKVAFTDSYMRNEQIIVVKKRSDIKTISDMKHKVLGAQSASSGYD
SLLRTPKLLKDFIKNKDANQYETFTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLENYRMIPTTFENEAFSVGLRKEDK
TLQAKINRAFRVLYQNGKFQAISEKWFGDDVATANIKS

Sequences:

>Translated_278_residues
MIIKKRTVAILAIASSFFLVACQATKSLKSGDAWGVYQKQKSITVGFDNTFVPMGYKDESGRCKGFDIDLAKEVFHQYGL
KVNFQAINWDMKEAELNNGKIDVIWNGYSITKERQDKVAFTDSYMRNEQIIVVKKRSDIKTISDMKHKVLGAQSASSGYD
SLLRTPKLLKDFIKNKDANQYETFTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLENYRMIPTTFENEAFSVGLRKEDK
TLQAKINRAFRVLYQNGKFQAISEKWFGDDVATANIKS
>Mature_278_residues
MIIKKRTVAILAIASSFFLVACQATKSLKSGDAWGVYQKQKSITVGFDNTFVPMGYKDESGRCKGFDIDLAKEVFHQYGL
KVNFQAINWDMKEAELNNGKIDVIWNGYSITKERQDKVAFTDSYMRNEQIIVVKKRSDIKTISDMKHKVLGAQSASSGYD
SLLRTPKLLKDFIKNKDANQYETFTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLENYRMIPTTFENEAFSVGLRKEDK
TLQAKINRAFRVLYQNGKFQAISEKWFGDDVATANIKS

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=270, Percent_Identity=27.4074074074074, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787085, Length=227, Percent_Identity=29.0748898678414, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787031, Length=233, Percent_Identity=25.7510729613734, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788648, Length=239, Percent_Identity=30.5439330543933, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788649, Length=233, Percent_Identity=28.3261802575107, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787088, Length=229, Percent_Identity=22.2707423580786, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 31741; Mature: 31741

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKKRTVAILAIASSFFLVACQATKSLKSGDAWGVYQKQKSITVGFDNTFVPMGYKDES
CEECCHHHEEEEEHHHHHEEEEHHHHCCCCCCCCCEEECCCEEEEECCCEEEECCCCCCC
GRCKGFDIDLAKEVFHQYGLKVNFQAINWDMKEAELNNGKIDVIWNGYSITKERQDKVAF
CCEECCCCHHHHHHHHHCCCEEEEEEECCCHHHHCCCCCEEEEEECCEEECCCCCCCEEE
TDSYMRNEQIIVVKKRSDIKTISDMKHKVLGAQSASSGYDSLLRTPKLLKDFIKNKDANQ
EHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHCCCCCCH
YETFTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLENYRMIPTTFENEAFSVGLRKEDK
HHHHHHHHHHHCCCCCCEEEEEEHHHCEEEEECCCCCCEEECCEEECCCEEEECCCCCCH
TLQAKINRAFRVLYQNGKFQAISEKWFGDDVATANIKS
HHHHHHHHHHHEEHCCCCEEEEHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MIIKKRTVAILAIASSFFLVACQATKSLKSGDAWGVYQKQKSITVGFDNTFVPMGYKDES
CEECCHHHEEEEEHHHHHEEEEHHHHCCCCCCCCCEEECCCEEEEECCCEEEECCCCCCC
GRCKGFDIDLAKEVFHQYGLKVNFQAINWDMKEAELNNGKIDVIWNGYSITKERQDKVAF
CCEECCCCHHHHHHHHHCCCEEEEEEECCCHHHHCCCCCEEEEEECCEEECCCCCCCEEE
TDSYMRNEQIIVVKKRSDIKTISDMKHKVLGAQSASSGYDSLLRTPKLLKDFIKNKDANQ
EHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHCCCCCCH
YETFTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLENYRMIPTTFENEAFSVGLRKEDK
HHHHHHHHHHHCCCCCCEEEEEEHHHCEEEEECCCCCCEEECCEEECCCEEEECCCCCCH
TLQAKINRAFRVLYQNGKFQAISEKWFGDDVATANIKS
HHHHHHHHHHHEEHCCCCEEEEHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]