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The map label for this gene is rsmF [H]

Identifier: 15675206

GI number: 15675206

Start: 1028228

End: 1029538

Strand: Reverse

Name: rsmF [H]

Synonym: SPy_1246

Alternate gene names: 15675206

Gene position: 1029538-1028228 (Counterclockwise)

Preceding gene: 15675207

Following gene: 15675205

Centisome position: 55.58

GC content: 40.58

Gene sequence:

>1311_bases
ATGATGTCATTACCAAAAGAATTTATTAACACTTATCAAGCAATACTAGGCAAAGAATTAGAAGATTTCTTGGCAAGTTT
TAACCAAGAGCCGGTCAATGCTTTTCGGATTAACCCCTTAAAGAATCAACTAAAAACCTTTGAACACCCCATTCCAAATA
CCTTATGGGGATATTATGGTAAACTATCTGGCAAGTCTCCAGAGCATGTGTCGGGGTTGGTTTATTCCCAAGAACCAGCG
GCTCAAATGGTGGCACAAGTAGCGGCTCCTCAAAAGGGGAGTCGTGTTTTAGACTTGGCAGCTGCACCAGGAGGAAAATC
AACTCATTTATTAGCTTATTTAGACAATACAGGTCTTTTAGTCAGCAATGAAATTTCTAAGAAGCGCAGCAAGGTTTTAG
TTGAAAATATCGAACGTTTTGGCGCTAGAAATGTTGTGGTGACTAATGAATCCGCAGATCGTTTAGCCAAGGTATTTTCT
CATTATTTTGATACTATCGTCTTTGATGGACCATGTTCTGGTGAAGGAATGTTTCGCAAGGACCCTGATGCTATTCAATA
TTGGCATCACGGGTATCCCGCAGAATGTGCCAAGTTGCAAAAATCTATTTTAGAAGACGCCTTAGCCATGTTAAAACCAG
GAGGAGAATTGATATACTCCACCTGTACTTGGGCTCCTGAAGAAAATGAAGATGTTGTGCAGTGGCTCTTAGAGACTTAT
ACCTTTTTAGAATTGGTCGATGTTCCAAAACTAAATGGAATGGTTAGTGGTATCGGACTTCCAGAGACTGCTAGAATGTA
CCCTCATCGTTATCAAGGGGAAGGGCAATTTGTGGCCAAGCTAAAAGATAAGCGTCAAGAAGGACAAAGCACTAAACTAA
AAGCACCTAAATCCAACTTGATCAAAGACCAGTTACGTCTTTGGAAAATGTTTGAAAAAGACCATCTAAACCTTACCCTA
TCAGGAACACTACAAACCTTTGGTGATTACCTATACCTCTTGCCAGATGGTCTTCCTGCTTTAGATGGCCTTAAAATTGC
TCGGAACGGTTTAGAATTAGGCGTCTTCAAGAAGAAACGCTTTGAACCCTCTTACGCCTTGGGATTAGCTTTAAAGCCTG
ATGAGGTCCGATCATCTATTGAGCTTAGAGAGGAAGACTTTAGACCTTATGTGTCAGGTAACGTCATTACTTTGCCCCAA
TCTTATCCAACAGGCTGGTACCAATTACTCATTAATGGCAATGGTCTAGGATTTGCCAAGGTCGTTGACAAGACCATCAA
AAATAATTTCCCTAAAGGGTTAAGATTTTAG

Upstream 100 bases:

>100_bases
AAACACGTCAAAAAATCATGTTTGTTCCTAAATGCCAACTCACTAGGATAGCGTCTTTTCTAACAAAGGACTCTGCCATC
ACACTTGAAGATCAATAGAA

Downstream 100 bases:

>100_bases
CGTAAAAAATAAGATTAAAAAGTTAAATATTTAAAGAAAAAATGCTTTCAAAGCATAGGTAGATATGTTACTATAAAGAA
TATGTGGCACTTAGTTTCTA

Product: putative nucleolar protein

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MMSLPKEFINTYQAILGKELEDFLASFNQEPVNAFRINPLKNQLKTFEHPIPNTLWGYYGKLSGKSPEHVSGLVYSQEPA
AQMVAQVAAPQKGSRVLDLAAAPGGKSTHLLAYLDNTGLLVSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFS
HYFDTIVFDGPCSGEGMFRKDPDAIQYWHHGYPAECAKLQKSILEDALAMLKPGGELIYSTCTWAPEENEDVVQWLLETY
TFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQFVAKLKDKRQEGQSTKLKAPKSNLIKDQLRLWKMFEKDHLNLTL
SGTLQTFGDYLYLLPDGLPALDGLKIARNGLELGVFKKKRFEPSYALGLALKPDEVRSSIELREEDFRPYVSGNVITLPQ
SYPTGWYQLLINGNGLGFAKVVDKTIKNNFPKGLRF

Sequences:

>Translated_436_residues
MMSLPKEFINTYQAILGKELEDFLASFNQEPVNAFRINPLKNQLKTFEHPIPNTLWGYYGKLSGKSPEHVSGLVYSQEPA
AQMVAQVAAPQKGSRVLDLAAAPGGKSTHLLAYLDNTGLLVSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFS
HYFDTIVFDGPCSGEGMFRKDPDAIQYWHHGYPAECAKLQKSILEDALAMLKPGGELIYSTCTWAPEENEDVVQWLLETY
TFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQFVAKLKDKRQEGQSTKLKAPKSNLIKDQLRLWKMFEKDHLNLTL
SGTLQTFGDYLYLLPDGLPALDGLKIARNGLELGVFKKKRFEPSYALGLALKPDEVRSSIELREEDFRPYVSGNVITLPQ
SYPTGWYQLLINGNGLGFAKVVDKTIKNNFPKGLRF
>Mature_436_residues
MMSLPKEFINTYQAILGKELEDFLASFNQEPVNAFRINPLKNQLKTFEHPIPNTLWGYYGKLSGKSPEHVSGLVYSQEPA
AQMVAQVAAPQKGSRVLDLAAAPGGKSTHLLAYLDNTGLLVSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFS
HYFDTIVFDGPCSGEGMFRKDPDAIQYWHHGYPAECAKLQKSILEDALAMLKPGGELIYSTCTWAPEENEDVVQWLLETY
TFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQFVAKLKDKRQEGQSTKLKAPKSNLIKDQLRLWKMFEKDHLNLTL
SGTLQTFGDYLYLLPDGLPALDGLKIARNGLELGVFKKKRFEPSYALGLALKPDEVRSSIELREEDFRPYVSGNVITLPQ
SYPTGWYQLLINGNGLGFAKVVDKTIKNNFPKGLRF

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=233, Percent_Identity=33.0472103004292, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI76150623, Length=233, Percent_Identity=33.0472103004292, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI32698918, Length=184, Percent_Identity=32.6086956521739, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI301336155, Length=197, Percent_Identity=29.9492385786802, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI39995082, Length=197, Percent_Identity=29.9492385786802, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI11545785, Length=170, Percent_Identity=31.7647058823529, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI270288818, Length=214, Percent_Identity=30.8411214953271, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI8922322, Length=217, Percent_Identity=31.3364055299539, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI23199998, Length=182, Percent_Identity=32.4175824175824, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI270288816, Length=217, Percent_Identity=31.3364055299539, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI87081985, Length=473, Percent_Identity=32.3467230443975, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI2367212, Length=300, Percent_Identity=28.6666666666667, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17536757, Length=307, Percent_Identity=31.9218241042345, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25143471, Length=206, Percent_Identity=32.0388349514563, Blast_Score=95, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6324268, Length=232, Percent_Identity=34.051724137931, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6319447, Length=210, Percent_Identity=30.952380952381, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI22024126, Length=233, Percent_Identity=33.4763948497854, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21355201, Length=393, Percent_Identity=25.4452926208651, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24668781, Length=195, Percent_Identity=29.7435897435897, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356513, Length=195, Percent_Identity=29.7435897435897, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920494, Length=156, Percent_Identity=32.6923076923077, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 48898; Mature: 48898

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSLPKEFINTYQAILGKELEDFLASFNQEPVNAFRINPLKNQLKTFEHPIPNTLWGYYG
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHCCCCCCHHHHHHC
KLSGKSPEHVSGLVYSQEPAAQMVAQVAAPQKGSRVLDLAAAPGGKSTHLLAYLDNTGLL
CCCCCCCCHHCCEEECCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEECCCCEE
VSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFSHYFDTIVFDGPCSGEGMFRK
EECHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHEEECCCCCCCCCCCC
DPDAIQYWHHGYPAECAKLQKSILEDALAMLKPGGELIYSTCTWAPEENEDVVQWLLETY
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHH
TFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQFVAKLKDKRQEGQSTKLKAPKSNL
HHHHHHCCCCCCCEEECCCCCHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCCHHHH
IKDQLRLWKMFEKDHLNLTLSGTLQTFGDYLYLLPDGLPALDGLKIARNGLELGVFKKKR
HHHHHHHHHHHHHCCCCEEEECCHHHHCCEEEECCCCCCCCCCHHHHHCCCCCCEEHHHC
FEPSYALGLALKPDEVRSSIELREEDFRPYVSGNVITLPQSYPTGWYQLLINGNGLGFAK
CCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEEEECCCCCHHH
VVDKTIKNNFPKGLRF
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMSLPKEFINTYQAILGKELEDFLASFNQEPVNAFRINPLKNQLKTFEHPIPNTLWGYYG
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHCCCCCCHHHHHHC
KLSGKSPEHVSGLVYSQEPAAQMVAQVAAPQKGSRVLDLAAAPGGKSTHLLAYLDNTGLL
CCCCCCCCHHCCEEECCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEECCCCEE
VSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFSHYFDTIVFDGPCSGEGMFRK
EECHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHEEECCCCCCCCCCCC
DPDAIQYWHHGYPAECAKLQKSILEDALAMLKPGGELIYSTCTWAPEENEDVVQWLLETY
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHH
TFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQFVAKLKDKRQEGQSTKLKAPKSNL
HHHHHHCCCCCCCEEECCCCCHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCCHHHH
IKDQLRLWKMFEKDHLNLTLSGTLQTFGDYLYLLPDGLPALDGLKIARNGLELGVFKKKR
HHHHHHHHHHHHHCCCCEEEECCHHHHCCEEEECCCCCCCCCCHHHHHCCCCCCEEHHHC
FEPSYALGLALKPDEVRSSIELREEDFRPYVSGNVITLPQSYPTGWYQLLINGNGLGFAK
CCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEEEECCCCCHHH
VVDKTIKNNFPKGLRF
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA