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The map label for this gene is clcA [H]

Identifier: 15675095

GI number: 15675095

Start: 912338

End: 913870

Strand: Direct

Name: clcA [H]

Synonym: SPy_1114

Alternate gene names: 15675095

Gene position: 912338-913870 (Clockwise)

Preceding gene: 15675094

Following gene: 15675096

Centisome position: 49.25

GC content: 36.59

Gene sequence:

>1533_bases
ATGGAAAACCATAAAAATGAATTCACCTTTTCCAACAAATCAATTATTGCCTATGTTTGGCGTGGTGTTGTGGTTGGTAT
TATTGCTGGTGTTATTGTTAGTTTATTCCGGTTGTTAATTGAAGTGACAGCTGACTGGGTGATTGAATGGTACCGATACG
CTCATATCAATTCGCTGTTGCTACTTCCTATACTAAGTGTCAGTTTGTTAGCTGTTCTTTTTGTAGGTTTTTTAGTAAAG
TCTGATTCTGATATAAAAGGATCAGGGATCCCCCATGTCGAAGGAGAACTAAAGGGGCTAATGTCCCCAGATTGGTGGAG
TGTCCTTTGGAAAAAATTTTTAGGAGGAATCATGGCTATTTCTATGGGATTTATGCTCGGTCGTGAGGGTCCTTCGATTC
AGTTAGGTGCTATGTCAGCCAAAGGATTAGCTAAGTTTTTAAAATCGAGTCGTTTGGAAAAGCGAGTGCTAATTGCCAGC
GGAGCAGCAGCAGGGCTATCAGCAGCTTTTAATGCTCCAATAGCAGGCCTCTTGTTTGTTGTTGAAGAAATTTACCATCA
TTTTTCGCGTTTAATTTGGATTACAGCGCTAGTGGCTAGCTTAGTGGCTAATTTTATTTCTCTGAATATTTTTGGATTGA
AACCAGTCTTAGCAATGTCAGAGGCGATGCCTTTTTTAGGACTCAATCAATATTGGTTGTTGCTTTTACTTGGATTATTT
CTAGGCTGCTTAGGCTATTTATATGAGATAGTTATCCTTAATTTTAATAAACTTTATGTTATTTTAGGGAGTTGGTTACA
TCTGCCAGATTATTTTTACGGTATTATTATGGTTTTTCTAATTTTACCGATTGGCTATTATTTACCACAGTTACTTGGAG
GAGGACATGGTTTAATACTTTCTTTATCCAATCAACAATTGCCTTTGATGACTATCTTCTTTTATTTTATTATTCGATTT
ATTGTTAGTATGTTTAGTTATGGAAGTGGCTTACCTGGAGGTATTTTTTTACCTATTTTAACTTTAGGTGCTTTGGCAGG
CCTATTATTTGGGCAAATAGCTTCTCAATTAGGTTTATTGAACCAATCTTTTCTTTCTCTTTTTTTGATATTAGGGATGG
CAGGGTATTTTGCGGCAATTTCAAAAGCACCACTGACAGGCATGATTTTGGTAACTGAAATGGTAGGTGATTTGAAACCC
TTAATGGCCATAGCTGTAGTTACTTTTGTCTCCTATTTAGTGATGGATTTGTTAAATGGACAGCCAATTTACGAAGCTAT
GCTAGATAAGATGGCAATGAAGCATCCAACTAATCTTGTTGAACCAACATTAATTGAATTGACTGTTGGTGATAAGATTG
CAGGGAAATATGTTAAAGAGTTAAAGCTTCCAGAAAATGTCTTAATTACAACCCAAATTCACCATCAAAAATCTCAAGTT
GTGTCAGGTAACACGCGCCTTTTATCAGGAGCAACTATCTTTTTGGTCGTTAATGAAGCTGATACAGGTTTTGTCCGTGA
GGTATTGATGTAG

Upstream 100 bases:

>100_bases
TTTTGGGTTACAGAAATCAGTTCTAAAAAAGAATCACTGGTAACTTAAGGTTGTCTTTGATACAATGGTAACAGTGTATT
TAAAAGAAAGAGAGGACAAC

Downstream 100 bases:

>100_bases
GTATTAATTTATCACAATTCGGAAAGTTCGAGCTTGTCTTTTAGACTAAGTCTTCGAACGTTTTACTGCTAGAGTTTCAT
CTGAACCAAAGTGAAACGCT

Product: hypothetical protein

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MENHKNEFTFSNKSIIAYVWRGVVVGIIAGVIVSLFRLLIEVTADWVIEWYRYAHINSLLLLPILSVSLLAVLFVGFLVK
SDSDIKGSGIPHVEGELKGLMSPDWWSVLWKKFLGGIMAISMGFMLGREGPSIQLGAMSAKGLAKFLKSSRLEKRVLIAS
GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLIWITALVASLVANFISLNIFGLKPVLAMSEAMPFLGLNQYWLLLLLGLF
LGCLGYLYEIVILNFNKLYVILGSWLHLPDYFYGIIMVFLILPIGYYLPQLLGGGHGLILSLSNQQLPLMTIFFYFIIRF
IVSMFSYGSGLPGGIFLPILTLGALAGLLFGQIASQLGLLNQSFLSLFLILGMAGYFAAISKAPLTGMILVTEMVGDLKP
LMAIAVVTFVSYLVMDLLNGQPIYEAMLDKMAMKHPTNLVEPTLIELTVGDKIAGKYVKELKLPENVLITTQIHHQKSQV
VSGNTRLLSGATIFLVVNEADTGFVREVLM

Sequences:

>Translated_510_residues
MENHKNEFTFSNKSIIAYVWRGVVVGIIAGVIVSLFRLLIEVTADWVIEWYRYAHINSLLLLPILSVSLLAVLFVGFLVK
SDSDIKGSGIPHVEGELKGLMSPDWWSVLWKKFLGGIMAISMGFMLGREGPSIQLGAMSAKGLAKFLKSSRLEKRVLIAS
GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLIWITALVASLVANFISLNIFGLKPVLAMSEAMPFLGLNQYWLLLLLGLF
LGCLGYLYEIVILNFNKLYVILGSWLHLPDYFYGIIMVFLILPIGYYLPQLLGGGHGLILSLSNQQLPLMTIFFYFIIRF
IVSMFSYGSGLPGGIFLPILTLGALAGLLFGQIASQLGLLNQSFLSLFLILGMAGYFAAISKAPLTGMILVTEMVGDLKP
LMAIAVVTFVSYLVMDLLNGQPIYEAMLDKMAMKHPTNLVEPTLIELTVGDKIAGKYVKELKLPENVLITTQIHHQKSQV
VSGNTRLLSGATIFLVVNEADTGFVREVLM
>Mature_510_residues
MENHKNEFTFSNKSIIAYVWRGVVVGIIAGVIVSLFRLLIEVTADWVIEWYRYAHINSLLLLPILSVSLLAVLFVGFLVK
SDSDIKGSGIPHVEGELKGLMSPDWWSVLWKKFLGGIMAISMGFMLGREGPSIQLGAMSAKGLAKFLKSSRLEKRVLIAS
GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLIWITALVASLVANFISLNIFGLKPVLAMSEAMPFLGLNQYWLLLLLGLF
LGCLGYLYEIVILNFNKLYVILGSWLHLPDYFYGIIMVFLILPIGYYLPQLLGGGHGLILSLSNQQLPLMTIFFYFIIRF
IVSMFSYGSGLPGGIFLPILTLGALAGLLFGQIASQLGLLNQSFLSLFLILGMAGYFAAISKAPLTGMILVTEMVGDLKP
LMAIAVVTFVSYLVMDLLNGQPIYEAMLDKMAMKHPTNLVEPTLIELTVGDKIAGKYVKELKLPENVLITTQIHHQKSQV
VSGNTRLLSGATIFLVVNEADTGFVREVLM

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Homo sapiens, GI153252026, Length=442, Percent_Identity=26.4705882352941, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI189217923, Length=428, Percent_Identity=26.4018691588785, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI189217921, Length=428, Percent_Identity=26.4018691588785, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4557473, Length=428, Percent_Identity=26.4018691588785, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1786350, Length=442, Percent_Identity=31.2217194570136, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI87081943, Length=321, Percent_Identity=28.9719626168224, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17532615, Length=466, Percent_Identity=24.2489270386266, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322500, Length=487, Percent_Identity=26.2833675564682, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24665008, Length=404, Percent_Identity=25.7425742574257, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45551566, Length=387, Percent_Identity=26.3565891472868, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 56022; Mature: 56022

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCEEEECEEEEEEEECCCCHHHHHHHC
>Mature Secondary Structure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HCCCCEEEECEEEEEEEECCCCHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA