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The map label for this gene is thiI

Identifier: 15674858

GI number: 15674858

Start: 673633

End: 674847

Strand: Direct

Name: thiI

Synonym: SPy_0817

Alternate gene names: 15674858

Gene position: 673633-674847 (Clockwise)

Preceding gene: 15674857

Following gene: 15674859

Centisome position: 36.36

GC content: 41.56

Gene sequence:

>1215_bases
ATGGACTATTCAGAAATTATGGTTCGCCATGGTGAACTATCAACTAAAGGGAAGAATCGCATGCGATTCATTAACAAACT
TAAAAACAATATTCAAGACGTACTGGCTCCTTTCCCAGCCATTACTGTCCGTTCAGATCGTGATAGGACCCATGTGTCTC
TAAATGGCACAGATTATCAGCCTATTGTAGAAGCTTTAAAGCTGGTGTTTGGTGTTCAAGCTCTATCTCCTGTTTATAAA
CTTGAAAAAAGTGTCCCTCTTTTGGTAACAGCTGTTCAAGATATCATGACTTCTCTTTATCGTGATGGACTGACGTTTAA
GATTGCCACTAAGCGTAGTGATCACGCGTTTGAATTGGATAGTCGTGAGCTTAACAGTCTTCTTGGTGGAGCTGTTTTTG
AGGTTTTACCAAATATCCAAGCCCAGATGAAACATCCCGATGTGACTCTCAAAGTCGAAATTCGTGATGAAGCAGCTTAC
ATTTCTTATGAAGAGATTAAGGGAGCAGGCGGGTTACCGGTAGGAACGTCAGGGAAAGGGATGCTGATGTTATCGGGCGG
TATTGATTCACCAGTAGCTGGGTATTTAGCCCTTAAACGTGGCCTTGATATTGAAGTTGTTCATTTTGCTAGCCCTCCGT
ACACCAGTCCAGGTGCCTTGGCTAAAGCCCAAGACCTTACTCGTCGATTAACTCGTTTTGGTGGAAATATCCAATTTATC
GAGGTTCCTTTTACAGAAATTCAAGAAGAAATTAAAAATAAGGCACCAGAGGCTTACCTTATGACATTAACCCGCCGCTT
TATGATGCGCATCACAGATGCCATTCGTGAGCAACGTAAGGGTCTTGTTATTGTTAACGGTGAGAGCCTAGGTCAGGTTG
CTAGTCAAACCCTTGAAAGCATGCAGGCCATTAATGCGGTAACATCAACTCCAATTATTAGACCAGTCGTGACAATGGAT
AAACTTGAAATTATCGAGATGGCTCAGGCGATTGATACTTTTGACATTTCTATTCAACCGTTCGAAGATTGCTGTACTAT
TTTTGCACCAGACCGTCCTAAAACCAACCCTAAACTAGGTAATGCTGAAAAATATGAAGAATGCTTTGATATTGATGGTC
TTGTCCAGCGTGCTGTGTCAGGAATTGTTGTCACAGAAATCACACCTGAAATCGTCAATGATGAGGTTGAAAATCTCATT
GATGCTTTACTTTAA

Upstream 100 bases:

>100_bases
TCTAGACGATGACAACGATATGGGACAAGTAGAGCAGTTTTTGACCATATTCCAAACAGATTTATGATAAAACACAGAAA
GTTAGGTAAGATTAGGTAAG

Downstream 100 bases:

>100_bases
AAGAAAAGATCAAGTGTAAATCTTTTAGTAGTCAGTAATGATACTATTGAAAAGCCTCTATAAGGGCTTTTTTGCTGCAA
TGAAGGAGGAAAATTTTGAG

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAITVRSDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYK
LEKSVPLLVTAVQDIMTSLYRDGLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
ISYEEIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPGALAKAQDLTRRLTRFGGNIQFI
EVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIREQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMD
KLEIIEMAQAIDTFDISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEECFDIDGLVQRAVSGIVVTEITPEIVNDEVENLI
DALL

Sequences:

>Translated_404_residues
MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAITVRSDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYK
LEKSVPLLVTAVQDIMTSLYRDGLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
ISYEEIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPGALAKAQDLTRRLTRFGGNIQFI
EVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIREQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMD
KLEIIEMAQAIDTFDISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEECFDIDGLVQRAVSGIVVTEITPEIVNDEVENLI
DALL
>Mature_404_residues
MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAITVRSDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYK
LEKSVPLLVTAVQDIMTSLYRDGLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
ISYEEIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPGALAKAQDLTRRLTRFGGNIQFI
EVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIREQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMD
KLEIIEMAQAIDTFDISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEECFDIDGLVQRAVSGIVVTEITPEIVNDEVENLI
DALL

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=384, Percent_Identity=28.90625, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_STRP1 (Q9A0D8)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269032.1
- RefSeq:   YP_281994.1
- ProteinModelPortal:   Q9A0D8
- SMR:   Q9A0D8
- EnsemblBacteria:   EBSTRT00000000380
- EnsemblBacteria:   EBSTRT00000028180
- GeneID:   3572299
- GeneID:   900989
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0817
- KEGG:   spz:M5005_Spy_0631
- GeneTree:   EBGT00050000027439
- HOGENOM:   HBG646072
- OMA:   ESIGQVA
- ProtClustDB:   PRK01565
- BioCyc:   SPYO160490:SPY0817-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0631-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 44731; Mature: 44731

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS51165 THUMP

Important sites: BINDING 265-265 BINDING 287-287 BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAITVRSDRDRTHVSLNGTDYQ
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCHH
PIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLYRDGLTFKIATKRSDHAFELD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECC
SRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAYISYEEIKGAGGLPVGTSGKG
HHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEECCCCEEEHHHHCCCCCCCCCCCCCE
MLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPGALAKAQDLTRRLTRFGGNIQFI
EEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
EVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIREQRKGLVIVNGESLGQVASQTLES
ECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHH
MQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTFDISIQPFEDCCTIFAPDRPKTNPKLG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCHHHHHEEECCCCCCCCCCCC
NAEKYEECFDIDGLVQRAVSGIVVTEITPEIVNDEVENLIDALL
CHHHHHHHHCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAITVRSDRDRTHVSLNGTDYQ
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCHH
PIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLYRDGLTFKIATKRSDHAFELD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECC
SRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAYISYEEIKGAGGLPVGTSGKG
HHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEECCCCEEEHHHHCCCCCCCCCCCCCE
MLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPGALAKAQDLTRRLTRFGGNIQFI
EEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
EVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIREQRKGLVIVNGESLGQVASQTLES
ECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHH
MQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTFDISIQPFEDCCTIFAPDRPKTNPKLG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCHHHHHEEECCCCCCCCCCCC
NAEKYEECFDIDGLVQRAVSGIVVTEITPEIVNDEVENLIDALL
CHHHHHHHHCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296