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The map label for this gene is folC.2 [H]

Identifier: 15674855

GI number: 15674855

Start: 670505

End: 671773

Strand: Reverse

Name: folC.2 [H]

Synonym: SPy_0814

Alternate gene names: 15674855

Gene position: 671773-670505 (Counterclockwise)

Preceding gene: 15674856

Following gene: 15674849

Centisome position: 36.26

GC content: 40.43

Gene sequence:

>1269_bases
ATGACTACCTATGATAGCATTTCCTGGATTCATACCTTTAAGGCTAATGGTCGCAAGACCGATTTGGCCAGAATGGCTTG
GTTACTTGAGGCACTAGGCCGGCCACAAGACAAATTCCCTGCTATTCACGTTGTTGGAACTAACGGCAAGGGCTCTGTGA
CAGCCTACTTACAGCATATCCTGTCAACTTCTGGTTATCAGGTCGGAACCTTTACATCTCCTTTTATTATCTCTTTTCAC
GATCGGATTTGCCTTAATGGCCAGCCCATTTCAGACCTAGAATTGGCGCAATGTGTGAGCGTGATTAGACCCATTCTCAC
TAAAATGTCTCTTGAAACAAATTGGGATAGACCTACCGAGTTTGAGTTAGTGACGCTCATAATGTTTTGCTACTTTGCCA
ATCTTAAACCTGTTGATATTGCCATTATTGAGGCAGGGATTGGTGGGAAAACCGACGCAACCAATGTTTTTCATGCTCTG
GCCGTCGTATGCCCTTCTATTAGTTTTGATCACCAAGAACGTTTAGGTTATAGCCTAGCAGAAATTGCTCACCAAAAAGC
AGAAGTTATCAAAGCTAAAGAACCCGTTATTATCGGTCAACTTGAGCAGGAGGCTAGTCAAGTCTTTCAACAAGTTACCC
AAAAAGCCGGTTCACGCTTATATCAATTAGGAAAAGATTTTTTGCTCAATCCTTCAGAAAAGACGTTTTCCTTTCAACAC
GATAACTTGAATTTAACCAAACTCCGTCTCAAACTATTAGGTCAACATCAAAAAGCGAATGCCTGTTTAGCCATTATGAC
AGCTCAATTACTTAGAAAATCCTTTCCTAAAATATCTCCAAAAACGATTCAAAAAGGAATTGAGGCAACAACTTGGCCTG
GCCGGTCCGAATTTATTCAACCTAATCTTCTATTAGACGGCGCTCACAATCCAGATTCCATTGCTAAATTAAAGTCTCTA
CTGCAAGAAGAATTTCCCAAACGTCCTATTCATATTCTCTTTGCAGGTCTCAAACGTAAGCCTTTAGCCGACTTGCTGGC
TCAACTAGATACTTTTGAAGTATCTGTCACGACTTTTGATTTCCCTGAAGCAAATCTTTTAGAGGATTATCCTGATAAAT
ACCCACAGGTAAAAGATTACAGAGACTGGTTACAAGGTTCCGTAACATCTGATGAACTCTTTGTTGTTACAGGGTCACTT
TATTTTATCTCCGAGGTAAGGCAATATTGTCTGAGCAACGCAAATATCTTTAAAAACATATTCCCCTAA

Upstream 100 bases:

>100_bases
CTGAAGGGACGCAAATTCCTAATGCAACTGGAGCTGAAGATGCTAATACTGGTCTTCCTACGAACCCTTAAAAGCATTGT
TAGAAGGACAAGAAATAGTA

Downstream 100 bases:

>100_bases
TCACCTAACAAAAAACGATGCTAGCAGTTTAGAAGACTTTCCTCAAAAACTGTTAACATCATTTTTTATTGCCTTAGGCT
ACATTTTTTAGCGCATGGTG

Product: putative folyl-polyglutamate synthetase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHVVGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFH
DRICLNGQPISDLELAQCVSVIRPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHAL
AVVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGSRLYQLGKDFLLNPSEKTFSFQH
DNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFPKISPKTIQKGIEATTWPGRSEFIQPNLLLDGAHNPDSIAKLKSL
LQEEFPKRPIHILFAGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDELFVVTGSL
YFISEVRQYCLSNANIFKNIFP

Sequences:

>Translated_422_residues
MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHVVGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFH
DRICLNGQPISDLELAQCVSVIRPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHAL
AVVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGSRLYQLGKDFLLNPSEKTFSFQH
DNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFPKISPKTIQKGIEATTWPGRSEFIQPNLLLDGAHNPDSIAKLKSL
LQEEFPKRPIHILFAGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDELFVVTGSL
YFISEVRQYCLSNANIFKNIFP
>Mature_421_residues
TTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHVVGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFHD
RICLNGQPISDLELAQCVSVIRPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHALA
VVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGSRLYQLGKDFLLNPSEKTFSFQHD
NLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFPKISPKTIQKGIEATTWPGRSEFIQPNLLLDGAHNPDSIAKLKSLL
QEEFPKRPIHILFAGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDELFVVTGSLY
FISEVRQYCLSNANIFKNIFP

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=320, Percent_Identity=30.9375, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI66932990, Length=320, Percent_Identity=30.9375, Blast_Score=112, Evalue=7e-25,
Organism=Escherichia coli, GI1788654, Length=311, Percent_Identity=29.5819935691318, Blast_Score=125, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17553148, Length=345, Percent_Identity=28.695652173913, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71984923, Length=345, Percent_Identity=28.695652173913, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17553150, Length=337, Percent_Identity=29.080118694362, Blast_Score=121, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6323760, Length=349, Percent_Identity=32.378223495702, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324815, Length=326, Percent_Identity=27.3006134969325, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322718, Length=432, Percent_Identity=25.2314814814815, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24641571, Length=326, Percent_Identity=30.6748466257669, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24581568, Length=327, Percent_Identity=25.0764525993884, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47422; Mature: 47291

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHVVGTNGKGSVTAYLQHI
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHH
LSTSGYQVGTFTSPFIISFHDRICLNGQPISDLELAQCVSVIRPILTKMSLETNWDRPTE
HHCCCCEEECCCCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
FELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHALAVVCPSISFDHQERLGYSLA
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCHHHH
EIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGSRLYQLGKDFLLNPSEKTFSFQH
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCEEEEC
DNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFPKISPKTIQKGIEATTWPGRSEFIQ
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCC
PNLLLDGAHNPDSIAKLKSLLQEEFPKRPIHILFAGLKRKPLADLLAQLDTFEVSVTTFD
CCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHCCEEEEEEEEC
FPEANLLEDYPDKYPQVKDYRDWLQGSVTSDELFVVTGSLYFISEVRQYCLSNANIFKNI
CCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHC
FP
CC
>Mature Secondary Structure 
TTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHVVGTNGKGSVTAYLQHI
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHH
LSTSGYQVGTFTSPFIISFHDRICLNGQPISDLELAQCVSVIRPILTKMSLETNWDRPTE
HHCCCCEEECCCCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
FELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHALAVVCPSISFDHQERLGYSLA
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCHHHH
EIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGSRLYQLGKDFLLNPSEKTFSFQH
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCEEEEC
DNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFPKISPKTIQKGIEATTWPGRSEFIQ
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCC
PNLLLDGAHNPDSIAKLKSLLQEEFPKRPIHILFAGLKRKPLADLLAQLDTFEVSVTTFD
CCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHCCEEEEEEEEC
FPEANLLEDYPDKYPQVKDYRDWLQGSVTSDELFVVTGSLYFISEVRQYCLSNANIFKNI
CCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHC
FP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]