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The map label for this gene is pepT

Identifier: 15674841

GI number: 15674841

Start: 658222

End: 659445

Strand: Direct

Name: pepT

Synonym: SPy_0799

Alternate gene names: 15674841

Gene position: 658222-659445 (Clockwise)

Preceding gene: 15674840

Following gene: 15674842

Centisome position: 35.53

GC content: 38.89

Gene sequence:

>1224_bases
ATGAAGTACGATAACTTGTTAGATCGATTTATAAAATATGTCAAAGTGAATACAAGAAGCGTTCCAGACAGTGAGACTAC
TCCTAGTACGGAGAGTCAAGAAGCATTTGCCTTAACGATTTTAAAGCCTGAAATGGAGGCAATTGGTTTGCAAGATGTTC
ATTATAATCCAGTTAATGGCTATCTTATTGGAACTCTGCCTGCCAATAATCCAACCTTGACGCGTAAAATTGGTTTTATA
GCCCATATGGATACGGCTGATTTCAATGCTGAAAATGTTAATCCGCAGATTATTGACAATTATCAAGGAGGGGACATTAC
ACTTGGCAGTTCTAATTATAAGTTGGATCCAAAAGCATTTCCAAATCTTAATAATTATATCGGTCAAACATTGATTACCA
CTGATGGGACTACACTTTTAGGGGCAGATGATAAATCTGGTATTGCTGAAATCATGACAGCAATAGAATTCTTGACATCA
CAACCACAAATAGAGCACTGTGACATTAAAGTGGCTTTTGGCCCTGATGAAGAGATTGGGGTCGGAGCTGATAAGTTTGA
GGTGGCTGATTTTGAGGTGGATTTTGCTTATACTATGGATGGCGGTCCTTTAGGCGAATTGCAATATGAAACTTTCAGCG
CTGCAGCTCTAGAAGTGACTTTTTTAGGCAGAAATGTTCATCCTGGTACGGCTAAGGATCAAATGATTAATGCCCTTGAG
TTAGCAATAGATTTTCATGAGAAGCTACCTGCTAAAGATCGGCCTGAATACACAGATGGTTATCAAGGTTTCTATCACTT
AACGGGACTAACTGGAACAGTTGAAGAGGCACGCGCATCTTACATTATTCGTGATTTTGAAGAAGCATCATTTGAAGCTA
GAAAAGTGAAAGTTGAAAATATAGCTCAATCGATGAATGCTCAGCTAGGAACAAAACGTGTCTTGGTAGAGCTTAATGAC
CAATACTACAATATGAAAAAAGTTATCGAAAAAGATATGACCGCTATTGAGTTAGCTAAAGAAGTAATGGAAGAGCTTGC
TATCAAGCCTGTAATCGAACCCATACGTGGAGGAACAGATGGCTCTAAAATTTCATTTATGGGTATTCCCACTCCTAATA
TTTTTGCAGGGGGTGAGAATATGCACGGGCGTTTTGAGTTTGTTAGCTTACAAACGATGGAGAGAGCTGTCGATGTCATT
ATTGGACTTGTTTGCAAAGCCTAA

Upstream 100 bases:

>100_bases
TCATTAAACTCTTAATTAGAGGCTTTAAAAAACTTAGCTTGAATAAGTATTTTAAAGCCTTTTATGCTATAATTAAATTG
AGAAATTATTGAGGTGGCCT

Downstream 100 bases:

>100_bases
TTATCATGGAATTGATATTTAGTGGAAGGGGCTTATAAAAATGATTAAACTATTTGGTAAAATAAGGTATCACTGGCAGC
CAGAGTTGTCTTGGTCTATT

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MKYDNLLDRFIKYVKVNTRSVPDSETTPSTESQEAFALTILKPEMEAIGLQDVHYNPVNGYLIGTLPANNPTLTRKIGFI
AHMDTADFNAENVNPQIIDNYQGGDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALEVTFLGRNVHPGTAKDQMINALE
LAIDFHEKLPAKDRPEYTDGYQGFYHLTGLTGTVEEARASYIIRDFEEASFEARKVKVENIAQSMNAQLGTKRVLVELND
QYYNMKKVIEKDMTAIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQTMERAVDVI
IGLVCKA

Sequences:

>Translated_407_residues
MKYDNLLDRFIKYVKVNTRSVPDSETTPSTESQEAFALTILKPEMEAIGLQDVHYNPVNGYLIGTLPANNPTLTRKIGFI
AHMDTADFNAENVNPQIIDNYQGGDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALEVTFLGRNVHPGTAKDQMINALE
LAIDFHEKLPAKDRPEYTDGYQGFYHLTGLTGTVEEARASYIIRDFEEASFEARKVKVENIAQSMNAQLGTKRVLVELND
QYYNMKKVIEKDMTAIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQTMERAVDVI
IGLVCKA
>Mature_407_residues
MKYDNLLDRFIKYVKVNTRSVPDSETTPSTESQEAFALTILKPEMEAIGLQDVHYNPVNGYLIGTLPANNPTLTRKIGFI
AHMDTADFNAENVNPQIIDNYQGGDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALEVTFLGRNVHPGTAKDQMINALE
LAIDFHEKLPAKDRPEYTDGYQGFYHLTGLTGTVEEARASYIIRDFEEASFEARKVKVENIAQSMNAQLGTKRVLVELND
QYYNMKKVIEKDMTAIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQTMERAVDVI
IGLVCKA

Specific function: Cleaves the N-terminal amino acid of tripeptides

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family

Homologues:

Organism=Escherichia coli, GI1787372, Length=400, Percent_Identity=50.25, Blast_Score=396, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPT_STRP1 (Q9A0F4)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269015.1
- RefSeq:   YP_281977.1
- ProteinModelPortal:   Q9A0F4
- SMR:   Q9A0F4
- EnsemblBacteria:   EBSTRT00000001395
- EnsemblBacteria:   EBSTRT00000027768
- GeneID:   3572282
- GeneID:   900972
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0799
- KEGG:   spz:M5005_Spy_0614
- GeneTree:   EBGT00050000027629
- HOGENOM:   HBG288638
- OMA:   HMDTAPD
- ProtClustDB:   PRK05469
- BioCyc:   SPYO160490:SPY0799-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0614-MONOMER
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00550
- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161
- PIRSF:   PIRSF037215
- TIGRFAMs:   TIGR01882

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.4.11.4

Molecular weight: Translated: 44969; Mature: 44969

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 84-84 ACT_SITE 177-177

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDNLLDRFIKYVKVNTRSVPDSETTPSTESQEAFALTILKPEMEAIGLQDVHYNPVNG
CCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHCCCCCCEECCCCC
YLIGTLPANNPTLTRKIGFIAHMDTADFNAENVNPQIIDNYQGGDITLGSSNYKLDPKAF
EEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCCCEEEECCCCCEEEECCCCCEECCHHC
PNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTSQPQIEHCDIKVAFGPDEEIG
CCHHHHHCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCC
VGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALEVTFLGRNVHPGTAKDQMINALE
CCCCCEEEEEEEEEEEEEECCCCCCCEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHH
LAIDFHEKLPAKDRPEYTDGYQGFYHLTGLTGTVEEARASYIIRDFEEASFEARKVKVEN
HHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHEEHHHH
IAQSMNAQLGTKRVLVELNDQYYNMKKVIEKDMTAIELAKEVMEELAIKPVIEPIRGGTD
HHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GSKISFMGIPTPNIFAGGENMHGRFEFVSLQTMERAVDVIIGLVCKA
CCEEEEEECCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKYDNLLDRFIKYVKVNTRSVPDSETTPSTESQEAFALTILKPEMEAIGLQDVHYNPVNG
CCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHCCCCCCEECCCCC
YLIGTLPANNPTLTRKIGFIAHMDTADFNAENVNPQIIDNYQGGDITLGSSNYKLDPKAF
EEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCCCEEEECCCCCEEEECCCCCEECCHHC
PNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTSQPQIEHCDIKVAFGPDEEIG
CCHHHHHCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCC
VGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALEVTFLGRNVHPGTAKDQMINALE
CCCCCEEEEEEEEEEEEEECCCCCCCEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHH
LAIDFHEKLPAKDRPEYTDGYQGFYHLTGLTGTVEEARASYIIRDFEEASFEARKVKVEN
HHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHEEHHHH
IAQSMNAQLGTKRVLVELNDQYYNMKKVIEKDMTAIELAKEVMEELAIKPVIEPIRGGTD
HHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GSKISFMGIPTPNIFAGGENMHGRFEFVSLQTMERAVDVIIGLVCKA
CCEEEEEECCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296