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The map label for this gene is lysS

Identifier: 15674679

GI number: 15674679

Start: 478041

End: 479534

Strand: Reverse

Name: lysS

Synonym: SPy_0595

Alternate gene names: 15674679

Gene position: 479534-478041 (Counterclockwise)

Preceding gene: 15674681

Following gene: 15674675

Centisome position: 25.89

GC content: 42.17

Gene sequence:

>1494_bases
ATGTCTAATCAACACATCGAAGAATTAAATGACCAACAGATTGTCCGTCGTGAAAAAATGACGGCGCTGGCTGAACAAGG
AATTGATCCTTTCGGTAAACGCTTTGACCGCACAGCCAATTCTGCTGAATTAAAAGAAAAGTATGCTGATAAAACAAAAG
AAGAATTACATGAGTTAAACGAAACAGCTATTGTTGCTGGCCGCTTGATGACAAAACGTGGCAAAGGTAAGGTTGGTTTT
GCTCATCTTCAAGACCGTGAAGGCCAAATCCAACTTTATGTTCGCAAGGACTCTGTTGGCGAAGACAACTATGAAATCTT
CAAAAAAGCCGATTTAGGCGACTTTATCGGCGTTGAAGGCGAAGTCATGCGTACAGACATGGGCGAGTTGTCAATCAAGG
CAACCAAGTTGACTCACCTTTCTAAATCACTTCGTCCTCTTCCTGAAAAATTCCACGGATTAACTGATATTGAAACCATT
TACCGTAAGCGTCACCTTGATTTAATTTCAAATCGTGAAAGCTTTGACCGCTTCGTGACCCGTTCCAAAATGATTTCAGA
AATCCGTCGTTATTTAGACGGTCTTGATTTCCTAGAAGTAGAAACGCCTGTGCTTCATAACGAGGCTGGTGGTGCTGCTG
CTCGTCCTTTCGTTACTCACCATAATGCTCAAAACATTGATATGGTGCTTCGTATCGCAACAGAACTTCATTTGAAACGT
TTGATTGTTGGTGGTATGGAACGTGTCTATGAAATTGGTCGTATCTTCCGTAACGAGGGAATGGATGCTACTCATAATCC
TGAGTTCACTTCTATTGAGGTTTACCAAGCCTATGCAGACTATCTTGATATCATGAACCTGACAGAAGGCATTATCCAAC
ATGCTGCTAAAGCTGTTAGAGGTGATGGCCCTATTGATTATCAAGGAACTGAAATCCGTATCAATGAACCTTTCAAACGT
GTTCACATGGTAGATGCTATCAAGGAAGTGACAGGTGCTGATTTCTGGCCTGAAATGACTGTCGAAGAAGCTATTGCACT
TGCCAAAGAAAAACAGGTCCCACTCGAAAAACACTTCATAAGTGTCGGACATATCATCAACGCTTTCTTCGAGGAATTTG
TTGAAGAAACCTTGGTCCAACCAACCTTTGTGTTTGGTCATCCTGTTGAAGTATCGCCTTTGGCTAAGAAGAATCCAGAA
GATACCCGCTTCACCGACCGCTTCGAACTTTTCATCATGACCAAAGAATACGCCAATGCTTTCACAGAGTTGAATGATCC
CATTGACCAATTATCTCGTTTTGAAGCACAAGCACAAGCTAAAGAACTTGGTGATGACGAAGCAACTGGCATTGACTACG
ACTTCGTTGAAGCTCTTGAATATGGTATGCCGCCAACCGGTGGACTCGGAATCGGGATTGATCGTCTTTGCATGTTATTA
ACAAACACAACAACAATCAGAGATGTGCTTTTATTCCCAACGATGAAACCTTAA

Upstream 100 bases:

>100_bases
CAAAAAAATGCCCCGCTGCAGTCGCATTTTCCAACGAAATATAGGTGTATTCTAGCCCCTTTTATGATACAATGGAAAGG
AAATCGTATTGGAGGAAAAT

Downstream 100 bases:

>100_bases
CTTTTTTAAGATTTGTATTCATATCTTAAAACCGATAAGTGGCAATATAACATAACGTTTTATTATCTCTAACCAGTCTT
CATTACCATGAAGACTGGTT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MSNQHIEELNDQQIVRREKMTALAEQGIDPFGKRFDRTANSAELKEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGF
AHLQDREGQIQLYVRKDSVGEDNYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETI
YRKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFVTHHNAQNIDMVLRIATELHLKR
LIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQAYADYLDIMNLTEGIIQHAAKAVRGDGPIDYQGTEIRINEPFKR
VHMVDAIKEVTGADFWPEMTVEEAIALAKEKQVPLEKHFISVGHIINAFFEEFVEETLVQPTFVFGHPVEVSPLAKKNPE
DTRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATGIDYDFVEALEYGMPPTGGLGIGIDRLCMLL
TNTTTIRDVLLFPTMKP

Sequences:

>Translated_497_residues
MSNQHIEELNDQQIVRREKMTALAEQGIDPFGKRFDRTANSAELKEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGF
AHLQDREGQIQLYVRKDSVGEDNYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETI
YRKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFVTHHNAQNIDMVLRIATELHLKR
LIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQAYADYLDIMNLTEGIIQHAAKAVRGDGPIDYQGTEIRINEPFKR
VHMVDAIKEVTGADFWPEMTVEEAIALAKEKQVPLEKHFISVGHIINAFFEEFVEETLVQPTFVFGHPVEVSPLAKKNPE
DTRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATGIDYDFVEALEYGMPPTGGLGIGIDRLCMLL
TNTTTIRDVLLFPTMKP
>Mature_496_residues
SNQHIEELNDQQIVRREKMTALAEQGIDPFGKRFDRTANSAELKEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGFA
HLQDREGQIQLYVRKDSVGEDNYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETIY
RKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFVTHHNAQNIDMVLRIATELHLKRL
IVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQAYADYLDIMNLTEGIIQHAAKAVRGDGPIDYQGTEIRINEPFKRV
HMVDAIKEVTGADFWPEMTVEEAIALAKEKQVPLEKHFISVGHIINAFFEEFVEETLVQPTFVFGHPVEVSPLAKKNPED
TRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATGIDYDFVEALEYGMPPTGGLGIGIDRLCMLLT
NTTTIRDVLLFPTMKP

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=508, Percent_Identity=42.1259842519685, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI194272210, Length=508, Percent_Identity=42.1259842519685, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI40789249, Length=284, Percent_Identity=24.6478873239437, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1789256, Length=510, Percent_Identity=49.4117647058824, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1790571, Length=497, Percent_Identity=49.8993963782696, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI87082379, Length=324, Percent_Identity=29.9382716049383, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1788173, Length=301, Percent_Identity=25.2491694352159, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=488, Percent_Identity=44.4672131147541, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71994340, Length=500, Percent_Identity=43.8, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17535927, Length=488, Percent_Identity=44.4672131147541, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI32566633, Length=297, Percent_Identity=25.5892255892256, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=492, Percent_Identity=44.7154471544715, Blast_Score=388, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324256, Length=504, Percent_Identity=33.7301587301587, Blast_Score=226, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6325153, Length=256, Percent_Identity=27.34375, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24640849, Length=487, Percent_Identity=43.7371663244353, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640851, Length=489, Percent_Identity=43.558282208589, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24584738, Length=310, Percent_Identity=25.4838709677419, Blast_Score=79, Evalue=8e-15,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYK_STRP1 (Q9A0V7)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_268853.1
- RefSeq:   YP_281858.1
- ProteinModelPortal:   Q9A0V7
- SMR:   Q9A0V7
- EnsemblBacteria:   EBSTRT00000001217
- EnsemblBacteria:   EBSTRT00000028353
- GeneID:   3572433
- GeneID:   900805
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0595
- KEGG:   spz:M5005_Spy_0495
- GeneTree:   EBGT00050000027607
- HOGENOM:   HBG631383
- OMA:   RNEGMDH
- ProtClustDB:   PRK00484
- BioCyc:   SPYO160490:SPY0595-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0495-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 56600; Mature: 56469

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQHIEELNDQQIVRREKMTALAEQGIDPFGKRFDRTANSAELKEKYADKTKEELHELN
CCCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ETAIVAGRLMTKRGKGKVGFAHLQDREGQIQLYVRKDSVGEDNYEIFKKADLGDFIGVEG
HHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHCCCCHHCCCCC
EVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETIYRKRHLDLISNRESFDRFVT
EEEECCCCHHEEEHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
RSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFVTHHNAQNIDMVLRIATELHLKR
HHHHHHHHHHHHCCCCEEECCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQAYADYLDIMNLTEGIIQHAAKAVR
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDGPIDYQGTEIRINEPFKRVHMVDAIKEVTGADFWPEMTVEEAIALAKEKQVPLEKHFI
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
SVGHIINAFFEEFVEETLVQPTFVFGHPVEVSPLAKKNPEDTRFTDRFELFIMTKEYANA
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEHHHHHH
FTELNDPIDQLSRFEAQAQAKELGDDEATGIDYDFVEALEYGMPPTGGLGIGIDRLCMLL
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TNTTTIRDVLLFPTMKP
HCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
SNQHIEELNDQQIVRREKMTALAEQGIDPFGKRFDRTANSAELKEKYADKTKEELHELN
CCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ETAIVAGRLMTKRGKGKVGFAHLQDREGQIQLYVRKDSVGEDNYEIFKKADLGDFIGVEG
HHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHCCCCHHCCCCC
EVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETIYRKRHLDLISNRESFDRFVT
EEEECCCCHHEEEHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
RSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFVTHHNAQNIDMVLRIATELHLKR
HHHHHHHHHHHHCCCCEEECCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQAYADYLDIMNLTEGIIQHAAKAVR
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDGPIDYQGTEIRINEPFKRVHMVDAIKEVTGADFWPEMTVEEAIALAKEKQVPLEKHFI
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
SVGHIINAFFEEFVEETLVQPTFVFGHPVEVSPLAKKNPEDTRFTDRFELFIMTKEYANA
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEHHHHHH
FTELNDPIDQLSRFEAQAQAKELGDDEATGIDYDFVEALEYGMPPTGGLGIGIDRLCMLL
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TNTTTIRDVLLFPTMKP
HCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296