Click here to switch to the map view.

The map label for this gene is cofE [H]

Identifier: 15674636

GI number: 15674636

Start: 437013

End: 437747

Strand: Direct

Name: cofE [H]

Synonym: SPy_0536

Alternate gene names: 15674636

Gene position: 437013-437747 (Clockwise)

Preceding gene: 15674635

Following gene: 15674637

Centisome position: 23.59

GC content: 33.61

Gene sequence:

>735_bases
ATGAATTTGAGTATATTTGGTTTAAAGAATATTCCTTATTTGAAAGAAGGGGATAGTATAGAAAAGCTTATTGAAGAAAG
CATTAAAACTTCAGAATTTTTTATCGAAGACAATGATGTGCTTTGCATAGCTTCTAAAGTTGTATCTATCGCAGAAGGCC
AGGTGATGTCTTTAAACGAGATACAAGTGAGTGATGTAGCAAAAGAAATCCACAGGAATATTCCTCGTAAAGATCCTAGA
ATAATTGAAATTATGCTAAATTTAGTCAATAGAGATCTGTCACGTTTAGATATCAAAAAAAATTACATTGGTTGTCGCTT
GGAAAATGGTTTAAAGTTGACAAGTGGTGGAATCGATAGAAAATCAGTAGATGAAGTATTTCTTTTACCTAATAATCCGG
ATGCTTCTGCAAAAAGAATTTCGGAATATCTTAAAAAATCACTTGGAAAAAATGTTGCAGTTGTTATAACAGATAGTGAT
GGTAGAGAGGATAAAAGAGGTGCTACTCAAGTAGCAATTGGTATTTATGGTATTCATCCTTTGAGAAAAACAGAAGTAAT
AGATTCACAAGGAGAGACAATAAAATTCCAAGAAGAGACTTTGTGTGATATGATTGCAGCTTGTGCTGGCTTAGTAATGG
GGCAACGTGGAACTGGAATTCCAGCTGTCCTCATTAGGGGCTTGGATTATAAATGGTCTGAAAATACTAGTATTAAGGAA
GCATTAAATGAGTAG

Upstream 100 bases:

>100_bases
ATTTTTAGTTTAGAAGTACATGAAAAAGAAATAGACAAAAAGGCAGAAGTAGTGAGGAAGTCTTATCTGACACTAAATGA
GTATATTAATGGAGGCTAAA

Downstream 100 bases:

>100_bases
AAATCAGGACTGTAAGAATATTCTAATCATTGGTTTTGGAAAAATTGGGAAAATTAAAGCGTACAAGTGGCTATCACGCG
GATATGAGGTATATGTCAAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MNLSIFGLKNIPYLKEGDSIEKLIEESIKTSEFFIEDNDVLCIASKVVSIAEGQVMSLNEIQVSDVAKEIHRNIPRKDPR
IIEIMLNLVNRDLSRLDIKKNYIGCRLENGLKLTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSD
GREDKRGATQVAIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGTGIPAVLIRGLDYKWSENTSIKE
ALNE

Sequences:

>Translated_244_residues
MNLSIFGLKNIPYLKEGDSIEKLIEESIKTSEFFIEDNDVLCIASKVVSIAEGQVMSLNEIQVSDVAKEIHRNIPRKDPR
IIEIMLNLVNRDLSRLDIKKNYIGCRLENGLKLTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSD
GREDKRGATQVAIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGTGIPAVLIRGLDYKWSENTSIKE
ALNE
>Mature_244_residues
MNLSIFGLKNIPYLKEGDSIEKLIEESIKTSEFFIEDNDVLCIASKVVSIAEGQVMSLNEIQVSDVAKEIHRNIPRKDPR
IIEIMLNLVNRDLSRLDIKKNYIGCRLENGLKLTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSD
GREDKRGATQVAIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGTGIPAVLIRGLDYKWSENTSIKE
ALNE

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [H]

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225 [H]

Pfam domain/function: PF01996 F420_ligase [H]

EC number: NA

Molecular weight: Translated: 27078; Mature: 27078

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSIFGLKNIPYLKEGDSIEKLIEESIKTSEFFIEDNDVLCIASKVVSIAEGQVMSLNE
CCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEHHHHHHHHCCCEEECCH
IQVSDVAKEIHRNIPRKDPRIIEIMLNLVNRDLSRLDIKKNYIGCRLENGLKLTSGGIDR
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHCEEEEEECCCEEECCCCCCC
KSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSDGREDKRGATQVAIGIYGIHP
CCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEEEEECCC
LRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGTGIPAVLIRGLDYKWSENTSIKE
CCHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCHHHH
ALNE
HHCC
>Mature Secondary Structure
MNLSIFGLKNIPYLKEGDSIEKLIEESIKTSEFFIEDNDVLCIASKVVSIAEGQVMSLNE
CCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEHHHHHHHHCCCEEECCH
IQVSDVAKEIHRNIPRKDPRIIEIMLNLVNRDLSRLDIKKNYIGCRLENGLKLTSGGIDR
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHCEEEEEECCCEEECCCCCCC
KSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSDGREDKRGATQVAIGIYGIHP
CCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEEEEECCC
LRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGTGIPAVLIRGLDYKWSENTSIKE
CCHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCHHHH
ALNE
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]