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The map label for this gene is vraB [H]

Identifier: 15674625

GI number: 15674625

Start: 422780

End: 423928

Strand: Direct

Name: vraB [H]

Synonym: SPy_0524

Alternate gene names: 15674625

Gene position: 422780-423928 (Clockwise)

Preceding gene: 15674624

Following gene: 15674626

Centisome position: 22.82

GC content: 44.21

Gene sequence:

>1149_bases
ATGACGGATGTTTATATTGCTGCAGGCCTTAGAACCCCTATCGGTTTAGTAGGGAAACAATTTGCTAAAGAACAACCAGA
AATCCTTGGAGCAAAGCTCATCAACGCTTTACAAAATAAGTATCCAGTTCCCATTGACCAAGTGATTTGTGGCAATACCG
TCGGAACGGGTGGTAATATTGGGCGCTTGATGACCCTGTATTCTCATTTAGGAGAATCTGTCTCTGCTTTGACGGTTGAT
ATGCAGTGTGCATCAGCTGGTGCAGCTCTTTCCGTGGGTTATGCTAAAATCAAGGCAGGTATGGCAAGCAACCTTTTAGT
GGGAGGGATTGAAAGCAGCTCCTTACAACCTGAATCGGTATATGCTTCAGCTGATTGGCGCCAAGGAGCCTACAAGGTAG
CTCAGTTTTCTCCAGATAGTATCAGCCCTTTTGCGATGATTGAAGGGGCAGAACGAGTGGCAAGAGAGCATGGCTTTACA
AAGGAGTATTTGAATCACTGGACATTGAGAAGTCATCAAAAGGCTAGTTATTGCCAGGAGCAAGCCCTGTTAGCTGATCT
TATTCTAGACCTGTCAGGAGCCAGTGATCAAGGTATTCGACCGCGCTTGTCAAGTAAGGTGTTGTCAAAGGTACCCCCTA
TCTTAGGAGAAGGTCACGTGATTAGTGCAGCCAATGCCTGCCTAACCCATGATGCTGCTGCCTTTTTACAGCTAAGTAGT
CAGCCTTCAGCTTTTAAACTGATTGATGTGGTAGAAGTAGCAGGAGACCCACAGCGTAGCCCTTTAATGGTGATTAAGGC
TAGTCAAGTCCTTTTGGAAAAACATGGTCTAGGGATGGCAGATATGACAGCGATTGAATGGAACGAAGCCTTTGCCGTTA
TTGATGGTCTATTTGAAACCCATTATCCAGATTTATTGGACCGCTATAATATATTTGGTGGTGCATTAGCTTATGGTCAT
CCCTATGGTGCCTCAGCAGCCATTATAATATTGCACTTAATGAGGGCTTTAGAGATTAAAAATGGACGCTATGGAATAGC
TGCTATAGCAGCAGCTGGTGGCCAAGGGTTTGCTGTTTTACTAAAATATCATAAGGAGTTTAGTCATGCTGACAAAGCTA
GAATATTGGGCAAAGCAATGCCCAAATAA

Upstream 100 bases:

>100_bases
TAGGTGTATTTTTAGCAGTTATTAGTTCAAAAAAGAAAACCATCAGAAATAATTATACAATAACCATTTAAGGACATCGA
AAATAAAGGAGGTCAATATA

Downstream 100 bases:

>100_bases
AAAAGCTATCGTAGCCGATCAGATAAGCTTGACTTATCAAGAGCTATGGCAAGCGGTTTTAATAAAGGATCAGACAATAA
AAGACAGTGTACCATATATC

Product: putative acetyl-CoA c-acetyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVPIDQVICGNTVGTGGNIGRLMTLYSHLGESVSALTVD
MQCASAGAALSVGYAKIKAGMASNLLVGGIESSSLQPESVYASADWRQGAYKVAQFSPDSISPFAMIEGAERVAREHGFT
KEYLNHWTLRSHQKASYCQEQALLADLILDLSGASDQGIRPRLSSKVLSKVPPILGEGHVISAANACLTHDAAAFLQLSS
QPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHGLGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAYGH
PYGASAAIIILHLMRALEIKNGRYGIAAIAAAGGQGFAVLLKYHKEFSHADKARILGKAMPK

Sequences:

>Translated_382_residues
MTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVPIDQVICGNTVGTGGNIGRLMTLYSHLGESVSALTVD
MQCASAGAALSVGYAKIKAGMASNLLVGGIESSSLQPESVYASADWRQGAYKVAQFSPDSISPFAMIEGAERVAREHGFT
KEYLNHWTLRSHQKASYCQEQALLADLILDLSGASDQGIRPRLSSKVLSKVPPILGEGHVISAANACLTHDAAAFLQLSS
QPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHGLGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAYGH
PYGASAAIIILHLMRALEIKNGRYGIAAIAAAGGQGFAVLLKYHKEFSHADKARILGKAMPK
>Mature_381_residues
TDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVPIDQVICGNTVGTGGNIGRLMTLYSHLGESVSALTVDM
QCASAGAALSVGYAKIKAGMASNLLVGGIESSSLQPESVYASADWRQGAYKVAQFSPDSISPFAMIEGAERVAREHGFTK
EYLNHWTLRSHQKASYCQEQALLADLILDLSGASDQGIRPRLSSKVLSKVPPILGEGHVISAANACLTHDAAAFLQLSSQ
PSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHGLGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAYGHP
YGASAAIIILHLMRALEIKNGRYGIAAIAAAGGQGFAVLLKYHKEFSHADKARILGKAMPK

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=423, Percent_Identity=27.6595744680851, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI167614485, Length=393, Percent_Identity=26.4631043256997, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI4501853, Length=392, Percent_Identity=26.7857142857143, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI148539872, Length=390, Percent_Identity=25.3846153846154, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4557237, Length=390, Percent_Identity=25.8974358974359, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1787663, Length=404, Percent_Identity=30.4455445544554, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI87082165, Length=400, Percent_Identity=29, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI48994986, Length=399, Percent_Identity=29.3233082706767, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788554, Length=391, Percent_Identity=28.3887468030691, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1788683, Length=412, Percent_Identity=24.7572815533981, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=28.2051282051282, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17535921, Length=393, Percent_Identity=29.2620865139949, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17551802, Length=428, Percent_Identity=26.6355140186916, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25147385, Length=379, Percent_Identity=25.5936675461741, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6325229, Length=403, Percent_Identity=27.5434243176179, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322031, Length=390, Percent_Identity=26.6666666666667, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24655093, Length=386, Percent_Identity=28.2383419689119, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=27.6346604215457, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI17648125, Length=393, Percent_Identity=26.2086513994911, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24640423, Length=394, Percent_Identity=25.1269035532995, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 40746; Mature: 40615

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVPIDQVICGNTVGTGGNI
CCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCH
GRLMTLYSHLGESVSALTVDMQCASAGAALSVGYAKIKAGMASNLLVGGIESSSLQPESV
HHHHHHHHHHCCCHHEEEEEHHHHCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCE
YASADWRQGAYKVAQFSPDSISPFAMIEGAERVAREHGFTKEYLNHWTLRSHQKASYCQE
EECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHH
QALLADLILDLSGASDQGIRPRLSSKVLSKVPPILGEGHVISAANACLTHDAAAFLQLSS
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHEECC
QPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHGLGMADMTAIEWNEAFAVIDGLFET
CCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHCCCCHHHEEEEEHHHHHHHHHHHHHH
HYPDLLDRYNIFGGALAYGHPYGASAAIIILHLMRALEIKNGRYGIAAIAAAGGQGFAVL
CCHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHH
LKYHKEFSHADKARILGKAMPK
HHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVPIDQVICGNTVGTGGNI
CCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCH
GRLMTLYSHLGESVSALTVDMQCASAGAALSVGYAKIKAGMASNLLVGGIESSSLQPESV
HHHHHHHHHHCCCHHEEEEEHHHHCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCE
YASADWRQGAYKVAQFSPDSISPFAMIEGAERVAREHGFTKEYLNHWTLRSHQKASYCQE
EECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHH
QALLADLILDLSGASDQGIRPRLSSKVLSKVPPILGEGHVISAANACLTHDAAAFLQLSS
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHEECC
QPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHGLGMADMTAIEWNEAFAVIDGLFET
CCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHCCCCHHHEEEEEHHHHHHHHHHHHHH
HYPDLLDRYNIFGGALAYGHPYGASAAIIILHLMRALEIKNGRYGIAAIAAAGGQGFAVL
CCHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHH
LKYHKEFSHADKARILGKAMPK
HHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA