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The map label for this gene is gloA [H]

Identifier: 15674615

GI number: 15674615

Start: 411103

End: 411480

Strand: Reverse

Name: gloA [H]

Synonym: SPy_0511

Alternate gene names: 15674615

Gene position: 411480-411103 (Counterclockwise)

Preceding gene: 15674616

Following gene: 15674614

Centisome position: 22.21

GC content: 37.83

Gene sequence:

>378_bases
ATGAAAGCATTACATACCTGTATCCGCGTCAAAGACCTTGACCAGTCGGTTGCTTTTTACACAAGTGCCTTCCCTTTTAA
GGAAAACTATCGTAAAGATTTTCCAGATAGTCAATTTACTCTTGTTTATCTGGCTTTAGAAGGAGAAAGTTATGAATTAG
AATTAACCTACAATTATGGTCATGGCGACTACGACTTAGGAAATGGCTATGGTCATATTGCCCTTGGCTCTGAACATTTT
GAAGCAGACCATAAAAAACATCGACAAGCAGGTTTTCCAGTTACCGATATCAAAGAACTGGCTGACAAATCTGCCCGCTA
TTATTTCATTCAGGATCCAGATGGCTACAAAATTGAAGTCATTGATTTAAACAACTAA

Upstream 100 bases:

>100_bases
AGCATGTTATCCCTAGGCTATCGTTTGCGAGATCCCAAACATGCTCAAGTTCGTAAGCCTAAAGAAGAAGTGATTTCAGT
CGTTAAATAAGGAGATTAAA

Downstream 100 bases:

>100_bases
TACTATTAACAACAGAAGCTTTAAAGCTTCTGTTGTTTTATTTTTTAACCTGACGATTGGGACAATCTCATTATTAACAG
AGTTTTGAAATATCTCAGCT

Product: putative lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYLALEGESYELELTYNYGHGDYDLGNGYGHIALGSEHF
EADHKKHRQAGFPVTDIKELADKSARYYFIQDPDGYKIEVIDLNN

Sequences:

>Translated_125_residues
MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYLALEGESYELELTYNYGHGDYDLGNGYGHIALGSEHF
EADHKKHRQAGFPVTDIKELADKSARYYFIQDPDGYKIEVIDLNN
>Mature_125_residues
MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYLALEGESYELELTYNYGHGDYDLGNGYGHIALGSEHF
EADHKKHRQAGFPVTDIKELADKSARYYFIQDPDGYKIEVIDLNN

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Escherichia coli, GI1787940, Length=125, Percent_Identity=38.4, Blast_Score=87, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14441; Mature: 14441

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYLALEGESYELELTYNYG
CCCHHHHEEEECCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEEECC
HGDYDLGNGYGHIALGSEHFEADHKKHRQAGFPVTDIKELADKSARYYFIQDPDGYKIEV
CCCCCCCCCEEEEEECCHHHCCHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCEEEE
IDLNN
EECCC
>Mature Secondary Structure
MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYLALEGESYELELTYNYG
CCCHHHHEEEECCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEEECC
HGDYDLGNGYGHIALGSEHFEADHKKHRQAGFPVTDIKELADKSARYYFIQDPDGYKIEV
CCCCCCCCCEEEEEECCHHHCCHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCEEEE
IDLNN
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]