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The map label for this gene is pmrA [H]

Identifier: 15674606

GI number: 15674606

Start: 402159

End: 403352

Strand: Direct

Name: pmrA [H]

Synonym: SPy_0501

Alternate gene names: 15674606

Gene position: 402159-403352 (Clockwise)

Preceding gene: 15674605

Following gene: 15674607

Centisome position: 21.71

GC content: 38.44

Gene sequence:

>1194_bases
GTGAACTGGCGACAAAATCTAAAAGTGGCTTGGCTTGGTAACTTCTTTACTGGGGCAAGCTTTTCACTTGTTATGCCTTT
TATGGCTCTATATGTGGAAAATTTAGGTACTCCGACAGAATTAGTAGAATACTATGCTGGTCTGGCAGTTGCTGTGACGG
CACTTGCTTCAGCTTTATTTGCTCCTGTTTGGGGGAAGTTGGCTGATCGTTATGGCCGAAAACCTATGATGCTGCGAGCC
AGTTTTGTTATGACATTTACAATGGGTGGTTTAGCTATTATTCCTAATGTGTTTTGGTTACTCATTTTGAGATTATTAAC
AGGTGTCTCTGCGGGCTATGTGCCAAATGCCACTGCCTTGATTGCCAGCCAAGCCCCAAAAGAGGAGTCAGGTTATGCTT
TAGGCACATTAGCAACAGGAGTGACAGCAGGCGCTTTAATTGGTCCTTTACTAGGGGGAATTTTGGCAGAGCTATTAGGA
ATTCGACAAGTTTTCTTATTAGTTGGTGTGATATTATTTTTATGTTCTTTAATGACAGCTGTTTATGTTAAAGAAGAGTT
TAAGCCGGTTAGGCGCTTTGAGATGATACCTACAAAAGTCATTTTAAAACAGGTAAAAAGTCCCCAGATTATGCTTGGTT
TATTTGTGACTAGTATGATTATTCAAATTTCAGCTCAATCAGTGGCCCCCATTTTATCATTGTATATTCGTCATCTTGGT
CAAACTCACAACTTAATGTTTACTTCAGGCCTGGTAGTTTCGGCCATGGGGTTTTCTAGCTTATTTAGCAGTTCTTACTT
AGGAAAATTAGGAGATCGATTTGGCAATCATCGGTTATTGTTAGCCGCTTTGTGTTATAGCTTTATCATGTATTTTAGCA
GTGCCTTAGCGCAGACCAGTTTTCAATTAGGAGTACTACGCTTTGCTTATGGGTTTGGTGTGGGCGCTTTGATGCCAAGT
ATTAACTCTTTACTAACTAAGTTAACACCAAAAGAAGGTATTTCTAGAGTATTTGCCTATAATCAAATGTTTAGTAACCT
TGGTCAAGTCATTGGCCCTTTTATTGGATCAAATGTGGCTGTAGTGTTAGGCTACCGTTCTGTTTTTTATGTGACTAGTT
TGATTGTGTTTGTTAATTTAATATGGAGTTTGATTATATTTCGAAAATATATTAAAGTTAAGGATATTGTGTGA

Upstream 100 bases:

>100_bases
CACTATTATGGGACACTGTTTTTGGCAGAAATGTGTTATAATAATCAAAGGCCCAATGGAAAGAAGATGATGATCTATTA
GTGATAAAGCGGTAACGACA

Downstream 100 bases:

>100_bases
GAGTAAAAATTAATTTAGAATGTAGTGAGTGTGGCAGTAACAATTATCTAACCAGTAAAAATAAAAGCAGTCATCCGGAA
AAAATAAAAGTTCCCAAATA

Product: putative multi-drug resistance efflux pump

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNWRQNLKVAWLGNFFTGASFSLVMPFMALYVENLGTPTELVEYYAGLAVAVTALASALFAPVWGKLADRYGRKPMMLRA
SFVMTFTMGGLAIIPNVFWLLILRLLTGVSAGYVPNATALIASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLG
IRQVFLLVGVILFLCSLMTAVYVKEEFKPVRRFEMIPTKVILKQVKSPQIMLGLFVTSMIIQISAQSVAPILSLYIRHLG
QTHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGNHRLLLAALCYSFIMYFSSALAQTSFQLGVLRFAYGFGVGALMPS
INSLLTKLTPKEGISRVFAYNQMFSNLGQVIGPFIGSNVAVVLGYRSVFYVTSLIVFVNLIWSLIIFRKYIKVKDIV

Sequences:

>Translated_397_residues
MNWRQNLKVAWLGNFFTGASFSLVMPFMALYVENLGTPTELVEYYAGLAVAVTALASALFAPVWGKLADRYGRKPMMLRA
SFVMTFTMGGLAIIPNVFWLLILRLLTGVSAGYVPNATALIASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLG
IRQVFLLVGVILFLCSLMTAVYVKEEFKPVRRFEMIPTKVILKQVKSPQIMLGLFVTSMIIQISAQSVAPILSLYIRHLG
QTHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGNHRLLLAALCYSFIMYFSSALAQTSFQLGVLRFAYGFGVGALMPS
INSLLTKLTPKEGISRVFAYNQMFSNLGQVIGPFIGSNVAVVLGYRSVFYVTSLIVFVNLIWSLIIFRKYIKVKDIV
>Mature_397_residues
MNWRQNLKVAWLGNFFTGASFSLVMPFMALYVENLGTPTELVEYYAGLAVAVTALASALFAPVWGKLADRYGRKPMMLRA
SFVMTFTMGGLAIIPNVFWLLILRLLTGVSAGYVPNATALIASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLG
IRQVFLLVGVILFLCSLMTAVYVKEEFKPVRRFEMIPTKVILKQVKSPQIMLGLFVTSMIIQISAQSVAPILSLYIRHLG
QTHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGNHRLLLAALCYSFIMYFSSALAQTSFQLGVLRFAYGFGVGALMPS
INSLLTKLTPKEGISRVFAYNQMFSNLGQVIGPFIGSNVAVVLGYRSVFYVTSLIVFVNLIWSLIIFRKYIKVKDIV

Specific function: Efflux pump for various substrates [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1787291, Length=391, Percent_Identity=44.2455242966752, Blast_Score=327, Evalue=7e-91,
Organism=Escherichia coli, GI87082404, Length=294, Percent_Identity=25.5102040816327, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 43357; Mature: 43357

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWRQNLKVAWLGNFFTGASFSLVMPFMALYVENLGTPTELVEYYAGLAVAVTALASALF
CCCHHCCEEEEECCHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
APVWGKLADRYGRKPMMLRASFVMTFTMGGLAIIPNVFWLLILRLLTGVSAGYVPNATAL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
IASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLGIRQVFLLVGVILFLCSLMTA
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYVKEEFKPVRRFEMIPTKVILKQVKSPQIMLGLFVTSMIIQISAQSVAPILSLYIRHLG
HHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QTHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGNHRLLLAALCYSFIMYFSSALAQTS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FQLGVLRFAYGFGVGALMPSINSLLTKLTPKEGISRVFAYNQMFSNLGQVIGPFIGSNVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VVLGYRSVFYVTSLIVFVNLIWSLIIFRKYIKVKDIV
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNWRQNLKVAWLGNFFTGASFSLVMPFMALYVENLGTPTELVEYYAGLAVAVTALASALF
CCCHHCCEEEEECCHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
APVWGKLADRYGRKPMMLRASFVMTFTMGGLAIIPNVFWLLILRLLTGVSAGYVPNATAL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
IASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLGIRQVFLLVGVILFLCSLMTA
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYVKEEFKPVRRFEMIPTKVILKQVKSPQIMLGLFVTSMIIQISAQSVAPILSLYIRHLG
HHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QTHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGNHRLLLAALCYSFIMYFSSALAQTS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FQLGVLRFAYGFGVGALMPSINSLLTKLTPKEGISRVFAYNQMFSNLGQVIGPFIGSNVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VVLGYRSVFYVTSLIVFVNLIWSLIIFRKYIKVKDIV
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9869592; 11463916 [H]