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The map label for this gene is glmU

Identifier: 15674567

GI number: 15674567

Start: 367158

End: 368540

Strand: Direct

Name: glmU

Synonym: SPy_0443

Alternate gene names: 15674567

Gene position: 367158-368540 (Clockwise)

Preceding gene: 15674566

Following gene: 15674568

Centisome position: 19.82

GC content: 43.09

Gene sequence:

>1383_bases
ATGACAAATTATGCCATTATTTTAGCAGCGGGCAAAGGGACTCGCATGACTTCTGATCTCCCAAAAGTTCTTCATAAAGT
ATCTGGACTAACCATGCTTGAGCATGTTTTTCGTAGTGTAAAAGCGATTAGTCCTGAAAAATCCGTGACGGTTATTGGTC
ACAAATCAGAAATGGTACGTGCTGTCTTAGCAGATCAATCAGCCTTTGTCCATCAAACAGAGCAGTTAGGAACTGGCCAT
GCCGTGATGATGGCAGAAACACAACTAGAAGGACTAGAAGGGCATACCTTGGTGATTGCAGGAGATACTCCCTTGATCAC
TGGAGAAAGCCTGAAGAGTCTGATTGATTTTCATGTGAATCATAAAAATGTCGCCACTATTTTAACAGCTACGGCTCAAG
ATCCATTTGGTTATGGTCGTATTGTTCGCAACAAAGATGGCGAAGTGATCAAGATTGTTGAGCAAAAAGACGCCAATGAG
TATGAGCAACAATTAAAAGAAATCAATACAGGAACCTATGTGTTTGATAACAAGCGTCTCTTTGAAGCGCTCAAATGCAT
CACTACCAACAATGCTCAAGGAGAGTATTACCTGACTGACGTTGTGGCTATTTTTAGAGCAAACAAAGAAAAGGTAGGAG
CCTATATCCTGAGAGACTTCAATGAAAGTCTGGGAGTAAATGATCGTGTGGCCTTAGCAATAGCTGAAACAGTGATGCGT
CAGCGCATTACCCAAAAACATATGGTCAATGGGGTGACTTTCCAAAATCCTGAAACTGTTTATATTGAAAGCGATGTTGA
GATTGCTCCAGACGTCCTTATCGAAGGAAATGTGACCTTAAAAGGGCGTACACATATCGGCTCAGGAACTGTCTTGACGA
ATGGTACTTATATTGTGGATTCTGAAATTGGTGATAACTGTGTTGTTACCAATTCGATGATCGAATCATCTGTTTTAGCT
GCAGGAGTTACGGTTGGCCCTTACGCCCACCTTCGACCGGGAACGACCTTAGATAGGGAAGTCCATATCGGTAACTTTGT
CGAGGTCAAGGGTTCTCACATTGGTGAAAAAACAAAGGCTGGGCATCTGACTTATATTGGAAACGCTCAGGTTGGGTCTT
CAGTCAATGTTGGGGCTGGAACCATCACTGTCAACTACGACGGCCAAAACAAATATGAAACAGTCATTGGAGATCACGCT
TTTATTGGGAGCAACTCGACTCTCATTGCACCTTTGGAAGTTGGGGATCACGCTTTAACAGCAGCAGGTTCAACGATTTC
AAAAACAGTTCCTATCGATAGCATTGCTATTGGTCGTAGCCGCCAAGTCACAAAAGAAGGTTATGCCAAGCGCCTAGCGC
ATCACCCAAGTCGAAGCAAATAA

Upstream 100 bases:

>100_bases
GACACTATAAAAGACAAGAAAATGCTAGGATGTTTCAGGAAAAAAAGAGAAAATATGCTATAATGAGAAGGTATTTAAAT
TATATAGTAAGGAAAAACAA

Downstream 100 bases:

>100_bases
TTCAAGATCTATTTAGTAAGGAGGCCAGGCATGAAATTTGAAGAAAAAACGCTTAAGAGACAAACAGTTTTTGATGGTCA
TATTTTTAAAGTTGTTGTGG

Product: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MTNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAISPEKSVTVIGHKSEMVRAVLADQSAFVHQTEQLGTGH
AVMMAETQLEGLEGHTLVIAGDTPLITGESLKSLIDFHVNHKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANE
YEQQLKEINTGTYVFDNKRLFEALKCITTNNAQGEYYLTDVVAIFRANKEKVGAYILRDFNESLGVNDRVALAIAETVMR
QRITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEGNVTLKGRTHIGSGTVLTNGTYIVDSEIGDNCVVTNSMIESSVLA
AGVTVGPYAHLRPGTTLDREVHIGNFVEVKGSHIGEKTKAGHLTYIGNAQVGSSVNVGAGTITVNYDGQNKYETVIGDHA
FIGSNSTLIAPLEVGDHALTAAGSTISKTVPIDSIAIGRSRQVTKEGYAKRLAHHPSRSK

Sequences:

>Translated_460_residues
MTNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAISPEKSVTVIGHKSEMVRAVLADQSAFVHQTEQLGTGH
AVMMAETQLEGLEGHTLVIAGDTPLITGESLKSLIDFHVNHKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANE
YEQQLKEINTGTYVFDNKRLFEALKCITTNNAQGEYYLTDVVAIFRANKEKVGAYILRDFNESLGVNDRVALAIAETVMR
QRITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEGNVTLKGRTHIGSGTVLTNGTYIVDSEIGDNCVVTNSMIESSVLA
AGVTVGPYAHLRPGTTLDREVHIGNFVEVKGSHIGEKTKAGHLTYIGNAQVGSSVNVGAGTITVNYDGQNKYETVIGDHA
FIGSNSTLIAPLEVGDHALTAAGSTISKTVPIDSIAIGRSRQVTKEGYAKRLAHHPSRSK
>Mature_459_residues
TNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAISPEKSVTVIGHKSEMVRAVLADQSAFVHQTEQLGTGHA
VMMAETQLEGLEGHTLVIAGDTPLITGESLKSLIDFHVNHKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANEY
EQQLKEINTGTYVFDNKRLFEALKCITTNNAQGEYYLTDVVAIFRANKEKVGAYILRDFNESLGVNDRVALAIAETVMRQ
RITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEGNVTLKGRTHIGSGTVLTNGTYIVDSEIGDNCVVTNSMIESSVLAA
GVTVGPYAHLRPGTTLDREVHIGNFVEVKGSHIGEKTKAGHLTYIGNAQVGSSVNVGAGTITVNYDGQNKYETVIGDHAF
IGSNSTLIAPLEVGDHALTAAGSTISKTVPIDSIAIGRSRQVTKEGYAKRLAHHPSRSK

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=454, Percent_Identity=42.7312775330396, Blast_Score=348, Evalue=4e-97,
Organism=Drosophila melanogaster, GI21355443, Length=350, Percent_Identity=23.7142857142857, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24644084, Length=350, Percent_Identity=23.7142857142857, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_STRP1 (Q9A163)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_268741.1
- RefSeq:   YP_281725.1
- ProteinModelPortal:   Q9A163
- SMR:   Q9A163
- EnsemblBacteria:   EBSTRT00000000414
- EnsemblBacteria:   EBSTRT00000027795
- GeneID:   3572534
- GeneID:   900690
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0443
- KEGG:   spz:M5005_Spy_0362
- GeneTree:   EBGT00050000026809
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- ProtClustDB:   PRK14354
- BioCyc:   SPYO160490:SPY0443-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0362-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 49690; Mature: 49559

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 362-362 BINDING 72-72 BINDING 139-139 BINDING 154-154 BINDING 169-169 BINDING 386-386 BINDING 404-404 BINDING 422-422 BINDING 439-439

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAISPEKSVTVIGHKSEMVR
CCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHH
AVLADQSAFVHQTEQLGTGHAVMMAETQLEGLEGHTLVIAGDTPLITGESLKSLIDFHVN
HHHHCCHHHHHHHHHCCCCCEEEEEECHHCCCCCCEEEEECCCCEECHHHHHHHHHHHCC
HKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANEYEQQLKEINTGTYVFDNKRL
CCCEEEEEEEECCCCCCCCEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCHHH
FEALKCITTNNAQGEYYLTDVVAIFRANKEKVGAYILRDFNESLGVNDRVALAIAETVMR
HHHHHHHCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
QRITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEGNVTLKGRTHIGSGTVLTNGTYIVD
HHHHHHHHHCCEEECCCCEEEEECCCCCCCCEEEECCEEEEECEECCCCEEEECCEEEEE
SEIGDNCVVTNSMIESSVLAAGVTVGPYAHLRPGTTLDREVHIGNFVEVKGSHIGEKTKA
CCCCCCEEEEHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEECCCEEEECCCCCCCCCCC
GHLTYIGNAQVGSSVNVGAGTITVNYDGQNKYETVIGDHAFIGSNSTLIAPLEVGDHALT
CEEEEEECCCCCCCEECCCEEEEEEECCCCCEEEEECCEEEECCCCCEEEEEECCCCEEE
AAGSTISKTVPIDSIAIGRSRQVTKEGYAKRLAHHPSRSK
ECCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAISPEKSVTVIGHKSEMVR
CCEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHH
AVLADQSAFVHQTEQLGTGHAVMMAETQLEGLEGHTLVIAGDTPLITGESLKSLIDFHVN
HHHHCCHHHHHHHHHCCCCCEEEEEECHHCCCCCCEEEEECCCCEECHHHHHHHHHHHCC
HKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANEYEQQLKEINTGTYVFDNKRL
CCCEEEEEEEECCCCCCCCEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCHHH
FEALKCITTNNAQGEYYLTDVVAIFRANKEKVGAYILRDFNESLGVNDRVALAIAETVMR
HHHHHHHCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
QRITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEGNVTLKGRTHIGSGTVLTNGTYIVD
HHHHHHHHHCCEEECCCCEEEEECCCCCCCCEEEECCEEEEECEECCCCEEEECCEEEEE
SEIGDNCVVTNSMIESSVLAAGVTVGPYAHLRPGTTLDREVHIGNFVEVKGSHIGEKTKA
CCCCCCEEEEHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEECCCEEEECCCCCCCCCCC
GHLTYIGNAQVGSSVNVGAGTITVNYDGQNKYETVIGDHAFIGSNSTLIAPLEVGDHALT
CEEEEEECCCCCCCEECCCEEEEEEECCCCCEEEEECCEEEECCCCCEEEEEECCCCEEE
AAGSTISKTVPIDSIAIGRSRQVTKEGYAKRLAHHPSRSK
ECCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296