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The map label for this gene is rsmI

Identifier: 15674543

GI number: 15674543

Start: 338928

End: 339791

Strand: Direct

Name: rsmI

Synonym: SPy_0406

Alternate gene names: 15674543

Gene position: 338928-339791 (Clockwise)

Preceding gene: 15674542

Following gene: 15674544

Centisome position: 18.3

GC content: 42.48

Gene sequence:

>864_bases
ATGCAGGTTCAAAAAAGTTTTAAAGACAAAAAAACAAGCGGAACCTTGTATTTGGTTCCAACCCCAATTGGCAATCTGCA
GGACATGACCTTTAGAGCAGTGGCAACGTTAAAAGAGGTTGATTTTATTTGTGCAGAAGACACAAGAAACACAGGTCTTT
TGCTAAAACACTTTGATATTGCTACCAAGCAAATCAGCTTTCATGAGCACAACGCCTATGAGAAGATTCCTGATTTAATT
GATTTATTAATATCAGGACGGTCACTAGCTCAGGTGTCTGATGCAGGAATGCCATCGATTTCTGATCCTGGGCATGATTT
GGTCAAGGCTGCTATTGATAGTGATATCGCAGTCGTTGCTTTACCAGGTGCCTCAGCAGGGATTACAGCTTTGATTGCTA
GTGGACTGGCTCCTCAACCCCATGTTTTTTATGGGTTTTTACCCAGAAAAGCAGGGCAACAAAAGGCTTTCTTTGAGGAC
AAACACCATTACCCAGAGACACAAATGTTTTATGAATCACCCTATCGCATCAAAGATACCCTGACCAATATGTTGGCTTG
TTATGGTGATCGTCAAGTGGTGCTTGTCAGAGAATTAACCAAGCTTTTTGAGGAGTACCAACGGGGGTCGATTTCAGAAA
TCTTGTCCTACCTTGAGGAAACACCCCTCAAAGGGGAGTGTCTGCTGATTGTGGCTGGAGCCCAAGCAGACAGTGAGGTT
GAGCTGACAGCTGATGTTGACTTAGTGTCTCTAGTGCAAAAAGAAATTCAAGCAGGAGCCAAGCCAAATCAGGCTATTAA
AACAATTGCTAAAGCTTACCAGGTAAATCGCCAAGAGCTTTACCAACAATTTCATGACTTATAA

Upstream 100 bases:

>100_bases
GATGAAGGGTTTCATATCTGCAATTTTTTCTATGGACAACGTCGTGAAAATGATGAAGAATGTATGTTTTGCAGAGAATT
ATTAGATAGAAAGTAAGGGC

Downstream 100 bases:

>100_bases
GTCATCGTAGATTAGGAGAAAAACAAATGACAGAAGATCAAGCAACTATCCTTATTTTAGGAGTATACGGAATCATATTG
GCTGTATTCTTATTATCAGC

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA 2'-O-ribose C1402 methyltransferase; rRNA (cytidine-2'-O-)-methyltransferase RsmI

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFICAEDTRNTGLLLKHFDIATKQISFHEHNAYEKIPDLI
DLLISGRSLAQVSDAGMPSISDPGHDLVKAAIDSDIAVVALPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFED
KHHYPETQMFYESPYRIKDTLTNMLACYGDRQVVLVRELTKLFEEYQRGSISEILSYLEETPLKGECLLIVAGAQADSEV
ELTADVDLVSLVQKEIQAGAKPNQAIKTIAKAYQVNRQELYQQFHDL

Sequences:

>Translated_287_residues
MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFICAEDTRNTGLLLKHFDIATKQISFHEHNAYEKIPDLI
DLLISGRSLAQVSDAGMPSISDPGHDLVKAAIDSDIAVVALPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFED
KHHYPETQMFYESPYRIKDTLTNMLACYGDRQVVLVRELTKLFEEYQRGSISEILSYLEETPLKGECLLIVAGAQADSEV
ELTADVDLVSLVQKEIQAGAKPNQAIKTIAKAYQVNRQELYQQFHDL
>Mature_287_residues
MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFICAEDTRNTGLLLKHFDIATKQISFHEHNAYEKIPDLI
DLLISGRSLAQVSDAGMPSISDPGHDLVKAAIDSDIAVVALPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFED
KHHYPETQMFYESPYRIKDTLTNMLACYGDRQVVLVRELTKLFEEYQRGSISEILSYLEETPLKGECLLIVAGAQADSEV
ELTADVDLVSLVQKEIQAGAKPNQAIKTIAKAYQVNRQELYQQFHDL

Specific function: Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA

COG id: COG0313

COG function: function code R; Predicted methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmI family

Homologues:

Organism=Escherichia coli, GI1789535, Length=250, Percent_Identity=44, Blast_Score=195, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMI_STRP1 (Q9A186)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_268717.1
- RefSeq:   YP_281699.1
- ProteinModelPortal:   Q9A186
- SMR:   Q9A186
- EnsemblBacteria:   EBSTRT00000001178
- EnsemblBacteria:   EBSTRT00000027656
- GeneID:   3572586
- GeneID:   900666
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0406
- KEGG:   spz:M5005_Spy_0335
- GeneTree:   EBGT00050000027471
- HOGENOM:   HBG529058
- OMA:   ASDRFCF
- ProtClustDB:   CLSK877046
- BioCyc:   SPYO160490:SPY0406-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0335-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01877
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR008189
- InterPro:   IPR018063
- Gene3D:   G3DSA:3.40.1010.10
- TIGRFAMs:   TIGR00096

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: NA

Molecular weight: Translated: 31819; Mature: 31819

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS01296 RSMI

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFICAEDTRNTGLLLKHFDI
CCCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEHH
ATKQISFHEHNAYEKIPDLIDLLISGRSLAQVSDAGMPSISDPGHDLVKAAIDSDIAVVA
HHHEECCHHCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEE
LPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFEDKHHYPETQMFYESPYRIKDT
ECCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCHHHHHCCCCHHHHH
LTNMLACYGDRQVVLVRELTKLFEEYQRGSISEILSYLEETPLKGECLLIVAGAQADSEV
HHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCE
ELTADVDLVSLVQKEIQAGAKPNQAIKTIAKAYQVNRQELYQQFHDL
EEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFICAEDTRNTGLLLKHFDI
CCCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEHH
ATKQISFHEHNAYEKIPDLIDLLISGRSLAQVSDAGMPSISDPGHDLVKAAIDSDIAVVA
HHHEECCHHCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEE
LPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFEDKHHYPETQMFYESPYRIKDT
ECCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCHHHHHCCCCHHHHH
LTNMLACYGDRQVVLVRELTKLFEEYQRGSISEILSYLEETPLKGECLLIVAGAQADSEV
HHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCE
ELTADVDLVSLVQKEIQAGAKPNQAIKTIAKAYQVNRQELYQQFHDL
EEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296