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The map label for this gene is murE

Identifier: 15674533

GI number: 15674533

Start: 330615

End: 332060

Strand: Reverse

Name: murE

Synonym: SPy_0388

Alternate gene names: 15674533

Gene position: 332060-330615 (Counterclockwise)

Preceding gene: 15674545

Following gene: 15674532

Centisome position: 17.93

GC content: 41.63

Gene sequence:

>1446_bases
ATGATAACCATTGAACAATTATTAGACATTTTAAAAAAAGACCATAATTTTCGTGAGGTTCTTGATGCCGACGGATACCA
CTACCATTATCAAGGCTTTAGTTTTGAGCGCTTGAGTTACGATAGCCGTCAAGTAGATGGCAAGACCCTTTTTTTTGCTA
AAGGGGCAACTTTCAAAGCTGACTACCTTAAAGAAGCAATCACTAACGGATTACAACTCTATATTTCAGAGGTAGATTAC
GAGCTTGGTATTCCCGTTGTTTTGGTCACTGATATCAAAAAAGCTATGAGTCTAATTGCCATGGCTTTCTATGGCAACCC
TCAAGAAAAGCTCAAACTACTAGCTTTTACAGGTACTAAGGGAAAGACGACGGCTGCTTATTTTGCCTACCACATGTTGA
AAGAATCCTATAAACCTGCCATGTTTTCTACCATGAATACCACCTTAGATGGCAAGACCTTTTTCAAATCACAACTCACA
ACTCCTGAGAGCTTGGATCTTTTTGCTATGATGGCTGAGTGTGTGACTAATGGCATGACCCACTTGATTATGGAAGTTTC
TAGTCAGGCTTACCTCGTTGACCGTGTCTATGGGCTTACGTTCGATGTTGGGGTTTTCCTAAATATTAGTCCTGATCATA
TTGGCCCAATCGAGCATCCAACGTTTGAAGATTATTTCTATCATAAGCGTCTTTTAATGGAGAATAGCAGAGCTGTTGTG
ATCAATAGCGGGATGGATCATTTTTCATTTCTAGCGGATCAGGTAGCGGATCAAGAGCATGTGTTTTATGGTCCCTTGTC
TGACAACCAGATCACCACTAGCCAAGCCTTCTCCTTTGAAGCTAAAGGACAATTAGCTGGCCATTATGACATTCAATTAA
TTGGCCATTTTAACCAAGAAAATGCTATGGCAGCTGGACTTGCCTGCCTTCGTTTGGGTGCTAGCCTAGCTGATATTCAA
AAAGGTATCGCTAAGACTCGCGTCCCAGGCCGTATGGAAGTCCTCACTATGACAAATCATGCTAAAGTTTTTGTTGATTA
TGCCCATAATGGTGACAGCTTAGAAAAATTACTCAGCGTTGTGGAAGAACATCAGACAGGCAAATTAATGTTGATTTTAG
GTGCACCAGGAAATAAAGGCGAGAGCAGACGAGCTGATTTTGGTAGGGTGATTCATCAGCATCCAAACCTCACCGTCATT
CTCACTGCTGATGACCCTAATTTCGAGGATCCAGAAGATATTTCAAAAGAAATTGCCAGCCATATCGCACGCCCAGTAGA
GATTATTAGTGACCGTGAGCAAGCTATTCAAAAAGCCATGTCTCTTTGCCAAGGAGCAAAAGATGCTGTGATTATCGCTG
GGAAAGGCGCAGATGCTTATCAAATTGTTAAAGGACAACAAGTAGCTTATGCTGGTGATTTAGCAATCGCCAAACACTAC
CTCTAG

Upstream 100 bases:

>100_bases
TTTCTGTCGACATATCTATTCTTTCCTAGTTTCTATTAGCTTTATTATAAACTTTTGCTTGTGACTTGTAAAACAAGAAC
TTTAAGGATAAAATAAGGGT

Downstream 100 bases:

>100_bases
AGTATGTTGACGTTAGTTTAGAATGATTATCGCTATTGTTTTTAATCATTCTAAACTAATATTTCTTTTTTAATACATCC
TAAAAATCACATCAAAAAAG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--L-lysine ligase

Products: NA

Alternate protein names: L-lysine-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MITIEQLLDILKKDHNFREVLDADGYHYHYQGFSFERLSYDSRQVDGKTLFFAKGATFKADYLKEAITNGLQLYISEVDY
ELGIPVVLVTDIKKAMSLIAMAFYGNPQEKLKLLAFTGTKGKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLT
TPESLDLFAMMAECVTNGMTHLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIEHPTFEDYFYHKRLLMENSRAVV
INSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQAFSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQ
KGIAKTRVPGRMEVLTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIHQHPNLTVI
LTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAVIIAGKGADAYQIVKGQQVAYAGDLAIAKHY
L

Sequences:

>Translated_481_residues
MITIEQLLDILKKDHNFREVLDADGYHYHYQGFSFERLSYDSRQVDGKTLFFAKGATFKADYLKEAITNGLQLYISEVDY
ELGIPVVLVTDIKKAMSLIAMAFYGNPQEKLKLLAFTGTKGKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLT
TPESLDLFAMMAECVTNGMTHLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIEHPTFEDYFYHKRLLMENSRAVV
INSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQAFSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQ
KGIAKTRVPGRMEVLTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIHQHPNLTVI
LTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAVIIAGKGADAYQIVKGQQVAYAGDLAIAKHY
L
>Mature_481_residues
MITIEQLLDILKKDHNFREVLDADGYHYHYQGFSFERLSYDSRQVDGKTLFFAKGATFKADYLKEAITNGLQLYISEVDY
ELGIPVVLVTDIKKAMSLIAMAFYGNPQEKLKLLAFTGTKGKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLT
TPESLDLFAMMAECVTNGMTHLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIEHPTFEDYFYHKRLLMENSRAVV
INSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQAFSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQ
KGIAKTRVPGRMEVLTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIHQHPNLTVI
LTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAVIIAGKGADAYQIVKGQQVAYAGDLAIAKHY
L

Specific function: Catalyzes the addition of L-lysine to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=497, Percent_Identity=29.3762575452716, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_STRP1 (Q9A196)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_268707.1
- RefSeq:   YP_281689.1
- ProteinModelPortal:   Q9A196
- SMR:   Q9A196
- EnsemblBacteria:   EBSTRT00000001312
- EnsemblBacteria:   EBSTRT00000028129
- GeneID:   3572576
- GeneID:   900656
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0388
- KEGG:   spz:M5005_Spy_0325
- GeneTree:   EBGT00050000027196
- HOGENOM:   HBG351099
- OMA:   GHEEGQT
- ProtClustDB:   PRK14022
- BioCyc:   SPYO160490:SPY0388-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0325-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.7

Molecular weight: Translated: 53514; Mature: 53514

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: BINDING 42-42 BINDING 158-158 BINDING 187-187 BINDING 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIEQLLDILKKDHNFREVLDADGYHYHYQGFSFERLSYDSRQVDGKTLFFAKGATFKA
CCCHHHHHHHHHCCCCHHHHHCCCCCEEEECCEEEHHCCCCCCCCCCCEEEEECCCCCHH
DYLKEAITNGLQLYISEVDYELGIPVVLVTDIKKAMSLIAMAFYGNPQEKLKLLAFTGTK
HHHHHHHHCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHEEEEEEECCC
GKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLTTPESLDLFAMMAECVTNGMT
CCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCHH
HLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIEHPTFEDYFYHKRLLMENSRAVV
HHHHHHCCCEEEEHHHHCEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
INSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQAFSFEAKGQLAGHYDIQLIGHFNQE
EECCCHHHHHHHHHHCCCCCEEECCCCCCCEECCCCEEEECCCCCCCEEEEEEEEECCCC
NAMAAGLACLRLGASLADIQKGIAKTRVPGRMEVLTMTNHAKVFVDYAHNGDSLEKLLSV
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCCHHHHHHHHH
VEEHQTGKLMLILGAPGNKGESRRADFGRVIHQHPNLTVILTADDPNFEDPEDISKEIAS
HHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHH
HIARPVEIISDREQAIQKAMSLCQGAKDAVIIAGKGADAYQIVKGQQVAYAGDLAIAKHY
HHCCHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCEEEEECCHHHHHCC
L
C
>Mature Secondary Structure
MITIEQLLDILKKDHNFREVLDADGYHYHYQGFSFERLSYDSRQVDGKTLFFAKGATFKA
CCCHHHHHHHHHCCCCHHHHHCCCCCEEEECCEEEHHCCCCCCCCCCCEEEEECCCCCHH
DYLKEAITNGLQLYISEVDYELGIPVVLVTDIKKAMSLIAMAFYGNPQEKLKLLAFTGTK
HHHHHHHHCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHEEEEEEECCC
GKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLTTPESLDLFAMMAECVTNGMT
CCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCHH
HLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIEHPTFEDYFYHKRLLMENSRAVV
HHHHHHCCCEEEEHHHHCEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
INSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQAFSFEAKGQLAGHYDIQLIGHFNQE
EECCCHHHHHHHHHHCCCCCEEECCCCCCCEECCCCEEEECCCCCCCEEEEEEEEECCCC
NAMAAGLACLRLGASLADIQKGIAKTRVPGRMEVLTMTNHAKVFVDYAHNGDSLEKLLSV
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCCHHHHHHHHH
VEEHQTGKLMLILGAPGNKGESRRADFGRVIHQHPNLTVILTADDPNFEDPEDISKEIAS
HHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHH
HIARPVEIISDREQAIQKAMSLCQGAKDAVIIAGKGADAYQIVKGQQVAYAGDLAIAKHY
HHCCHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCEEEEECCHHHHHCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296