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The map label for this gene is gidB

Identifier: 15674490

GI number: 15674490

Start: 288438

End: 289151

Strand: Reverse

Name: gidB

Synonym: SPy_0329

Alternate gene names: 15674490

Gene position: 289151-288438 (Counterclockwise)

Preceding gene: 15674505

Following gene: 15674489

Centisome position: 15.61

GC content: 38.24

Gene sequence:

>714_bases
ATGACACCCCAAGACTTTTACCGAACACTAGAGGAAGATGGTTTTTCCTTATCATCGAAACAAAAAGAACAATTTGACAC
CTACTTCAAATCACTAGTGGAATGGAACACTAAAATTAATTTAACAGCTATTACAGAAGAAAATGAGGTTTACCTCAAGC
ATTTTTATGATTCCATCGCTCCTATTTTACAGGGCTTTTTAGCTAATGAACCAATCAAATTATTGGACATTGGAGCTGGA
GCTGGTTTTCCTAGCCTACCCATGAAAATTCTTTTCCCAAACCTTGAGGTTACCATTATTGATTCTCTTAATAAGCGGAT
TTCTTTTCTGACACTCCTTGCTCAGGAATTGGGCTTGGAAAATGTCCACTTTTTTCATGGTAGAGCAGAAGATTTCGGAC
AGGACAAGGCCTTTCGAGGTCAATTTGATGTTGTTACTGCCAGAGCGGTTGCTCGGATGCAGGTTCTATCTGAATTAACC
ATCCCCTTTTTAAAAATTGGAGGAAAATTAATCGCCTTAAAGGCACAAGCTGCTGACCAAGAGTTAGAAGAAGCTAAGAA
TGCTCTTTGTCTTCTTTTTGGTAAAGTCATTAAAAACCATAGCTACCAATTGCCAAATGGAGATTCTCGCTTCATCACCA
TTGTTGAGAAGAAAAAGGAAACACCTAACAAGTATCCAAGAAAAGCTGGCTTGCCTAACAAAAAACCTCTATAA

Upstream 100 bases:

>100_bases
AATAGCAAGTAATGAGGTGTTATTTCTGATGATTTATTGGATTTTGTTATAGGCTATTTTATGATAGAATGGTAACAACT
ATCAAAAACAGAGGATACAA

Downstream 100 bases:

>100_bases
AATAGTACCAAAGGCTAAGATGAAACTTAGCTTTTTTAACTTATTTTTGTTATTATAGTAGGAGATATTATGTCTGGAGG
AAACATGAAACGCTCTTTTA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MTPQDFYRTLEEDGFSLSSKQKEQFDTYFKSLVEWNTKINLTAITEENEVYLKHFYDSIAPILQGFLANEPIKLLDIGAG
AGFPSLPMKILFPNLEVTIIDSLNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELT
IPFLKIGGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKKKETPNKYPRKAGLPNKKPL

Sequences:

>Translated_237_residues
MTPQDFYRTLEEDGFSLSSKQKEQFDTYFKSLVEWNTKINLTAITEENEVYLKHFYDSIAPILQGFLANEPIKLLDIGAG
AGFPSLPMKILFPNLEVTIIDSLNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELT
IPFLKIGGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKKKETPNKYPRKAGLPNKKPL
>Mature_236_residues
TPQDFYRTLEEDGFSLSSKQKEQFDTYFKSLVEWNTKINLTAITEENEVYLKHFYDSIAPILQGFLANEPIKLLDIGAGA
GFPSLPMKILFPNLEVTIIDSLNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELTI
PFLKIGGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKKKETPNKYPRKAGLPNKKPL

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=175, Percent_Identity=34.8571428571429, Blast_Score=106, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_STRP1 (Q9A1D7)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_268664.1
- RefSeq:   YP_281642.1
- ProteinModelPortal:   Q9A1D7
- SMR:   Q9A1D7
- EnsemblBacteria:   EBSTRT00000000342
- EnsemblBacteria:   EBSTRT00000028041
- GeneID:   3572646
- GeneID:   900612
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0329
- KEGG:   spz:M5005_Spy_0278
- GeneTree:   EBGT00050000027008
- HOGENOM:   HBG686577
- OMA:   AITHPNS
- ProtClustDB:   PRK00107
- BioCyc:   SPYO160490:SPY0329-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0278-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 26780; Mature: 26649

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQDFYRTLEEDGFSLSSKQKEQFDTYFKSLVEWNTKINLTAITEENEVYLKHFYDSIA
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHH
PILQGFLANEPIKLLDIGAGAGFPSLPMKILFPNLEVTIIDSLNKRISFLTLLAQELGLE
HHHHHHHCCCCEEEEEECCCCCCCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHCCC
NVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELTIPFLKIGGKLIALKAQAADQ
CEEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHH
ELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKKKETPNKYPRKAGLPNKKPL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TPQDFYRTLEEDGFSLSSKQKEQFDTYFKSLVEWNTKINLTAITEENEVYLKHFYDSIA
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHH
PILQGFLANEPIKLLDIGAGAGFPSLPMKILFPNLEVTIIDSLNKRISFLTLLAQELGLE
HHHHHHHCCCCEEEEEECCCCCCCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHCCC
NVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELTIPFLKIGGKLIALKAQAADQ
CEEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHH
ELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKKKETPNKYPRKAGLPNKKPL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296