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The map label for this gene is thiN [H]

Identifier: 15674445

GI number: 15674445

Start: 229124

End: 229756

Strand: Direct

Name: thiN [H]

Synonym: SPy_0265

Alternate gene names: 15674445

Gene position: 229124-229756 (Clockwise)

Preceding gene: 15674444

Following gene: 15674446

Centisome position: 12.37

GC content: 40.44

Gene sequence:

>633_bases
ATGTCTAAAGTTGCTCTTTTTGCGGGAGGAGATCTCTCTTATATCAGCCGTGATTTTGATTATTTTGTCGGTATTGATAG
AGGGAGCCTATTTTTATTAGAAAATGGGCTTCCTTTGAATATGGCTGTTGGTGATTTTGATTCGGTTTCCCAAAAAGCGT
TTACGGATATAAAAGAGAAGGCAGAGCTTTTTATTACTGCTCATCCAGAAAAAAATGATACGGATACGGAATTAGCCCTT
AAAGAGGTCTTTGCTCGTTTTCCTGAGGCGGAAGTGACCATTTTTGGGGCTTTTGGGGGTCGAATGGACCACCTCTTATC
CAATATTTTCTTGCCTAGCGACCCTGGTATTGCTCCCTTTATGGCACAAATCGCCTTGCGTGACCAACAAAATATGATTA
CCTACCGCCCGGCAGGTCAGCACCTTATCCATCAAGAAGAAGGGATGACCTATGTGGCATTTATGGCAGAAGGAGAAGCT
GACTTGACCATTACTGGTGCTAAATTTGAGTTGACGCAAGATAATTTTTTCAAAAAGAAAATCTATTCCAGTAATGCCTT
TATCCATCAACCTATTACAGTTAGTTTGCCAAGTGGCTACTTGATTATTATTCAAAGTAAAGATTGGAGTTAG

Upstream 100 bases:

>100_bases
GCTTGTTATGAAGCAGGGGCCAATGTCTTTGTGGCAGGTTCTTACCTTTTCAAGGCCAGTGACCTAGTGAGCCAAGTCCA
AACCTTAAGGACGGCCTTGA

Downstream 100 bases:

>100_bases
TATGGACCTTATCTTGTTCCTTTTGGTCTTGGTTCTCTTAGGTTTAGGGGCTTATCTGTTGTTCAAAGTCAACGGCCTTC
AACATCAGCTTGCCCAAACC

Product: hypothetical protein

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MSKVALFAGGDLSYISRDFDYFVGIDRGSLFLLENGLPLNMAVGDFDSVSQKAFTDIKEKAELFITAHPEKNDTDTELAL
KEVFARFPEAEVTIFGAFGGRMDHLLSNIFLPSDPGIAPFMAQIALRDQQNMITYRPAGQHLIHQEEGMTYVAFMAEGEA
DLTITGAKFELTQDNFFKKKIYSSNAFIHQPITVSLPSGYLIIIQSKDWS

Sequences:

>Translated_210_residues
MSKVALFAGGDLSYISRDFDYFVGIDRGSLFLLENGLPLNMAVGDFDSVSQKAFTDIKEKAELFITAHPEKNDTDTELAL
KEVFARFPEAEVTIFGAFGGRMDHLLSNIFLPSDPGIAPFMAQIALRDQQNMITYRPAGQHLIHQEEGMTYVAFMAEGEA
DLTITGAKFELTQDNFFKKKIYSSNAFIHQPITVSLPSGYLIIIQSKDWS
>Mature_209_residues
SKVALFAGGDLSYISRDFDYFVGIDRGSLFLLENGLPLNMAVGDFDSVSQKAFTDIKEKAELFITAHPEKNDTDTELALK
EVFARFPEAEVTIFGAFGGRMDHLLSNIFLPSDPGIAPFMAQIALRDQQNMITYRPAGQHLIHQEEGMTYVAFMAEGEAD
LTITGAKFELTQDNFFKKKIYSSNAFIHQPITVSLPSGYLIIIQSKDWS

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 23419; Mature: 23288

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVALFAGGDLSYISRDFDYFVGIDRGSLFLLENGLPLNMAVGDFDSVSQKAFTDIKEK
CCEEEEEECCCHHHHHCCCEEEEEECCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHCC
AELFITAHPEKNDTDTELALKEVFARFPEAEVTIFGAFGGRMDHLLSNIFLPSDPGIAPF
EEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHHH
MAQIALRDQQNMITYRPAGQHLIHQEEGMTYVAFMAEGEADLTITGAKFELTQDNFFKKK
HHHHHHCCCCCEEEECCCCCCEEECCCCCEEEEEEECCCCCEEEECCEEEECCCCHHHHH
IYSSNAFIHQPITVSLPSGYLIIIQSKDWS
HHCCCCEEECCEEEECCCCEEEEEECCCCC
>Mature Secondary Structure 
SKVALFAGGDLSYISRDFDYFVGIDRGSLFLLENGLPLNMAVGDFDSVSQKAFTDIKEK
CEEEEEECCCHHHHHCCCEEEEEECCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHCC
AELFITAHPEKNDTDTELALKEVFARFPEAEVTIFGAFGGRMDHLLSNIFLPSDPGIAPF
EEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHHH
MAQIALRDQQNMITYRPAGQHLIHQEEGMTYVAFMAEGEADLTITGAKFELTQDNFFKKK
HHHHHHCCCCCEEEECCCCCCEEECCCCCEEEEEEECCCCCEEEECCEEEECCCCHHHHH
IYSSNAFIHQPITVSLPSGYLIIIQSKDWS
HHCCCCEEECCEEEECCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]