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The map label for this gene is gltX [H]

Identifier: 15674424

GI number: 15674424

Start: 208437

End: 209882

Strand: Direct

Name: gltX [H]

Synonym: SPy_0239

Alternate gene names: 15674424

Gene position: 208437-209882 (Clockwise)

Preceding gene: 15674423

Following gene: 15674425

Centisome position: 11.25

GC content: 41.15

Gene sequence:

>1446_bases
ATGTCTAAACCAATTCGTGTGCGTTATGCACCAAGTCCAACAGGGCTTTTACATATCGGGAATGCCCGCACAGCCCTATT
TAATTACCTTTATGCCCGTCGTCACGGAGGTACTTTTATCATTCGTATTGAAGATACTGACCGTAAACGTCATGTTGAAG
ATGGTGAACGTTCTCAACTTGAAAACCTAAAATGGTTAGGAATGGATTGGGATGAAAGCCCAGAAACGCATGAAAATTAC
CGCCAGTCTGAACGTCTAGCACTTTATCAACAATATATTGATCAATTGTTAGCTGAAGGAAAAGCCTACAAGTCTTACGT
GACTGAGGAAGAATTGGCAGCAGAGCGTGAACGCCAAGAAGCAGCTGGCGAAACGCCTCGCTATATCAATGAATTTATTG
GTATGTCAGCTGACGAAAAAGCTAAGTACATTGCTGAGCGTGAAGCAGCAGGTATCGTTCCAACAGTCCGTCTTGCTGTT
AATGAGTCCGGTATTTACAAATGGACTGACATGGTTAAAGGCGATATCGAGTTTGAAGGTGGTAACATCGGTGGTGACTG
GGTTATTCAAAAGAAAGATGGCTATCCAACTTATAACTTTGCCGTTGTTGTTGATGATCATGATATGCAAATTTCTCACG
TTATCCGTGGCGATGACCATATTGCCAATACGCCAAAACAATTGATGGTCTATGAAGCTCTTGGCTGGGAAGCGCCAGAG
TTTGGTCATATGACTCTTATCATCAATTCTGAAACGGGTAAAAAATTATCAAAACGCGACACCAATACCCTCCAATTCAT
TGAAGATTACCGCAAAAAAGGTTATATGCCTGAAGCTGTCTTTAACTTCATCGCCCTTCTTGGCTGGAATCCTGGCGGAG
AAGAGGAAATCTTCTCTCGTGAGCAACTGATTGCCCTTTTTGATGAAAATCGCCTTAGCAAGTCGCCAGCTGCCTTTGAC
CAGAAGAAAATGGATTGGATGAGCAATGAGTATCTTAAACATGCGGATTTTGAAACAGTTTATGCCCTCTGCAAACCATT
CCTTGAAGAGGCAGGCCGTTTGACAGAAAAAGCTGAGAAGCTAGTAGAATTATACAAACCTCAATTAAAATCTGCAGATG
AGATTATTCCATTGACTGACCTTTTCTTTTCTGATTTCCCTGAATTAACAGAAGCCGAAAAAGAAGTGATGGCAGGTGAA
ACAGTTTCGACAGTCTTGCAAGCTTTCAAGGCTAAGTTGGAAGCTATGTCAGATGAAGACTTCAAACCAGAAAATATTTT
CCCACAAATTAAGGCTGTTCAAAAAGAAACAGGAATCAAAGGGAAAAACCTCTTTATGCCTATTCGTATTGCAGTATCTG
GTGAAATGCATGGCCCAGAATTACCAAATACGATTTATTTATTGGGACGTGATAAATCCATTGAGCACATCAAAAACATG
CTATAA

Upstream 100 bases:

>100_bases
AGAAGAAGTCTGAAAAGGTCTTGGCAAGCTCAAATCATCATTTGTTGCTTGTTTTTCGTGCCACAGGTTTCTGAGTGATA
TATGTAACAGGAGAAGAAGC

Downstream 100 bases:

>100_bases
GGCTGTATCGTTCACCTTTATTTTTAAACGCTTTAAGGAGTTAAAAAGTCACGACAACGAAAGATTGAAGATTAAGTTCT
TCAGTCTTTTTTATCGTTAT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIEDTDRKRHVEDGERSQLENLKWLGMDWDESPETHENY
RQSERLALYQQYIDQLLAEGKAYKSYVTEEELAAERERQEAAGETPRYINEFIGMSADEKAKYIAEREAAGIVPTVRLAV
NESGIYKWTDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPE
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMPEAVFNFIALLGWNPGGEEEIFSREQLIALFDENRLSKSPAAFD
QKKMDWMSNEYLKHADFETVYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEAEKEVMAGE
TVSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPNTIYLLGRDKSIEHIKNM
L

Sequences:

>Translated_481_residues
MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIEDTDRKRHVEDGERSQLENLKWLGMDWDESPETHENY
RQSERLALYQQYIDQLLAEGKAYKSYVTEEELAAERERQEAAGETPRYINEFIGMSADEKAKYIAEREAAGIVPTVRLAV
NESGIYKWTDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPE
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMPEAVFNFIALLGWNPGGEEEIFSREQLIALFDENRLSKSPAAFD
QKKMDWMSNEYLKHADFETVYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEAEKEVMAGE
TVSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPNTIYLLGRDKSIEHIKNM
L
>Mature_480_residues
SKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIEDTDRKRHVEDGERSQLENLKWLGMDWDESPETHENYR
QSERLALYQQYIDQLLAEGKAYKSYVTEEELAAERERQEAAGETPRYINEFIGMSADEKAKYIAEREAAGIVPTVRLAVN
ESGIYKWTDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPEF
GHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMPEAVFNFIALLGWNPGGEEEIFSREQLIALFDENRLSKSPAAFDQ
KKMDWMSNEYLKHADFETVYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEAEKEVMAGET
VSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPNTIYLLGRDKSIEHIKNML

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=495, Percent_Identity=30.9090909090909, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI62241042, Length=258, Percent_Identity=25.968992248062, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI4826960, Length=298, Percent_Identity=25.1677852348993, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788743, Length=480, Percent_Identity=37.9166666666667, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI226510925, Length=253, Percent_Identity=30.4347826086957, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71993394, Length=492, Percent_Identity=30.6910569105691, Blast_Score=211, Evalue=8e-55,
Organism=Caenorhabditis elegans, GI17543332, Length=298, Percent_Identity=25.8389261744966, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324540, Length=341, Percent_Identity=39.2961876832845, Blast_Score=235, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6321192, Length=218, Percent_Identity=27.9816513761468, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=309, Percent_Identity=23.9482200647249, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24665321, Length=505, Percent_Identity=30.6930693069307, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI17864488, Length=300, Percent_Identity=27, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 55094; Mature: 54963

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIEDTDRKRHVEDGERSQL
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCHHCCCCCCHHHH
ENLKWLGMDWDESPETHENYRQSERLALYQQYIDQLLAEGKAYKSYVTEEELAAERERQE
HCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHH
AAGETPRYINEFIGMSADEKAKYIAEREAAGIVPTVRLAVNESGIYKWTDMVKGDIEFEG
HCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEHHHCCCEEEECC
GNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPE
CCCCCCEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHCCCHHHHEEEHHHCCCCCC
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMPEAVFNFIALLGWNPGGEEEIFSR
CCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHH
EQLIALFDENRLSKSPAAFDQKKMDWMSNEYLKHADFETVYALCKPFLEEAGRLTEKAEK
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LVELYKPQLKSADEIIPLTDLFFSDFPELTEAEKEVMAGETVSTVLQAFKAKLEAMSDED
HHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
FKPENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPNTIYLLGRDKSIEHIKNM
CCCCCCCHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEEECCCCHHHHHHC
L
C
>Mature Secondary Structure 
SKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIEDTDRKRHVEDGERSQL
CCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCHHCCCCCCHHHH
ENLKWLGMDWDESPETHENYRQSERLALYQQYIDQLLAEGKAYKSYVTEEELAAERERQE
HCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHH
AAGETPRYINEFIGMSADEKAKYIAEREAAGIVPTVRLAVNESGIYKWTDMVKGDIEFEG
HCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEHHHCCCEEEECC
GNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGDDHIANTPKQLMVYEALGWEAPE
CCCCCCEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHCCCHHHHEEEHHHCCCCCC
FGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYMPEAVFNFIALLGWNPGGEEEIFSR
CCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHH
EQLIALFDENRLSKSPAAFDQKKMDWMSNEYLKHADFETVYALCKPFLEEAGRLTEKAEK
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LVELYKPQLKSADEIIPLTDLFFSDFPELTEAEKEVMAGETVSTVLQAFKAKLEAMSDED
HHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
FKPENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPNTIYLLGRDKSIEHIKNM
CCCCCCCHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEEECCCCHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA