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The map label for this gene is insK [H]

Identifier: 15674400

GI number: 15674400

Start: 182123

End: 182929

Strand: Reverse

Name: insK [H]

Synonym: SPy_0198

Alternate gene names: 15674400

Gene position: 182929-182123 (Counterclockwise)

Preceding gene: 15674401

Following gene: 15674398

Centisome position: 9.88

GC content: 39.53

Gene sequence:

>807_bases
ATGCTACTTGAAATCCTTGATTTATCACGCTCCACCTATTACTATCAAGTGAAGCGACTAGCTCAAGGAGATAAGGATAT
AGAACTAAAGCATGTGATTCGAGAGATTTATGATGAGCATAAAGGTAATTACGGTTATCGTCGGATTCATATGGAGTTGC
GTAACCGAGGATTTGTGGTCAATCATAAGAAAGTTCAACGTCTCATGAAAGTCATGGGCTTAGCAGCCCGCATTCGTCGG
AAGCGCAAGTATTCTTCTTACAAAGGAGAGGTTGGTAAGAAGGCTGATAATCTGATTAAACGTCACTTCGAAGGCTCTAA
ACCTTACGAAAAATGTTACACTGATGTGACGGAGTTGGCCTTACCTGAAGGAAAACTCTACTTATCACCTGTTCTTGATG
GCTATAACAGTGAGATTATTGATTTCACTCTATCTCGGTCTCCTAACTTGAAACAGGTTCAAACCATGCTTGAGAAGACT
TTTCCAGCAGATTCATACAGCGGAACTATTCTTCATAGTGACCAGGGGTGGCAATACCAACACCAGTCCTACCATGATTT
TTTAGAGTCTAAAGGTATTCTGGCATCCATGTCCCGCAAAGGGAATAGTCCGGATAACGGCATGATGGAATCCTTCTTTG
GCATTCTCAAATCTGAAATGTTTTATGGACTCGAGACAACTTATCAATCACTTGACAAGCTTGAAGAAGCTATTACAGAT
TACATTTTTTACTACAACAACAAACGAATTAAAGCAAAACTAAAAGGATTTAGTCCTGTCCAATACAGAACTAAATCCTT
TCAATAA

Upstream 100 bases:

>100_bases
CTGTGCTAAAAAAGCTGAGAGAATACCGCTTGAGGGACGAAGCAAAACTCAAAGAGCAACAGAAATCATTCAAGCATTAA
GGAATCAATTTCCCTTAGAG

Downstream 100 bases:

>100_bases
TTATTTGTCCAACTTTTTGGGGTCAGTACACTCCTAGTTAACGACTTTGATTGATTTTATTATGATGAATCGTGATTTCT
GAAAACAAAATTTTCTTCAT

Product: putative transposase, IS861

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MLLEILDLSRSTYYYQVKRLAQGDKDIELKHVIREIYDEHKGNYGYRRIHMELRNRGFVVNHKKVQRLMKVMGLAARIRR
KRKYSSYKGEVGKKADNLIKRHFEGSKPYEKCYTDVTELALPEGKLYLSPVLDGYNSEIIDFTLSRSPNLKQVQTMLEKT
FPADSYSGTILHSDQGWQYQHQSYHDFLESKGILASMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLDKLEEAITD
YIFYYNNKRIKAKLKGFSPVQYRTKSFQ

Sequences:

>Translated_268_residues
MLLEILDLSRSTYYYQVKRLAQGDKDIELKHVIREIYDEHKGNYGYRRIHMELRNRGFVVNHKKVQRLMKVMGLAARIRR
KRKYSSYKGEVGKKADNLIKRHFEGSKPYEKCYTDVTELALPEGKLYLSPVLDGYNSEIIDFTLSRSPNLKQVQTMLEKT
FPADSYSGTILHSDQGWQYQHQSYHDFLESKGILASMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLDKLEEAITD
YIFYYNNKRIKAKLKGFSPVQYRTKSFQ
>Mature_268_residues
MLLEILDLSRSTYYYQVKRLAQGDKDIELKHVIREIYDEHKGNYGYRRIHMELRNRGFVVNHKKVQRLMKVMGLAARIRR
KRKYSSYKGEVGKKADNLIKRHFEGSKPYEKCYTDVTELALPEGKLYLSPVLDGYNSEIIDFTLSRSPNLKQVQTMLEKT
FPADSYSGTILHSDQGWQYQHQSYHDFLESKGILASMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLDKLEEAITD
YIFYYNNKRIKAKLKGFSPVQYRTKSFQ

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=254, Percent_Identity=27.5590551181102, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1789981, Length=267, Percent_Identity=47.1910112359551, Blast_Score=254, Evalue=4e-69,
Organism=Escherichia coli, GI1788405, Length=272, Percent_Identity=28.3088235294118, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787263, Length=272, Percent_Identity=28.3088235294118, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786753, Length=272, Percent_Identity=28.3088235294118, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786570, Length=272, Percent_Identity=28.3088235294118, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786490, Length=272, Percent_Identity=28.3088235294118, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31465; Mature: 31465

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEILDLSRSTYYYQVKRLAQGDKDIELKHVIREIYDEHKGNYGYRRIHMELRNRGFVV
CEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEE
NHKKVQRLMKVMGLAARIRRKRKYSSYKGEVGKKADNLIKRHFEGSKPYEKCYTDVTELA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LPEGKLYLSPVLDGYNSEIIDFTLSRSPNLKQVQTMLEKTFPADSYSGTILHSDQGWQYQ
CCCCCEEEHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHH
HQSYHDFLESKGILASMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLDKLEEAITD
HHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIFYYNNKRIKAKLKGFSPVQYRTKSFQ
HHHHCCCCEEEEEECCCCCHHHHCCCCC
>Mature Secondary Structure
MLLEILDLSRSTYYYQVKRLAQGDKDIELKHVIREIYDEHKGNYGYRRIHMELRNRGFVV
CEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEE
NHKKVQRLMKVMGLAARIRRKRKYSSYKGEVGKKADNLIKRHFEGSKPYEKCYTDVTELA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LPEGKLYLSPVLDGYNSEIIDFTLSRSPNLKQVQTMLEKTFPADSYSGTILHSDQGWQYQ
CCCCCEEEHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHH
HQSYHDFLESKGILASMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLDKLEEAITD
HHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIFYYNNKRIKAKLKGFSPVQYRTKSFQ
HHHHCCCCEEEEEECCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]