| Definition | Streptococcus pyogenes M1 GAS chromosome, complete genome. |
|---|---|
| Accession | NC_002737 |
| Length | 1,852,441 |
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The map label for this gene is polA [H]
Identifier: 15674390
GI number: 15674390
Start: 171524
End: 174166
Strand: Direct
Name: polA [H]
Synonym: SPy_0185
Alternate gene names: 15674390
Gene position: 171524-174166 (Clockwise)
Preceding gene: 15674389
Following gene: 15674391
Centisome position: 9.26
GC content: 42.34
Gene sequence:
>2643_bases ATGGAAAACAAGAATAAATTACTATTAATTGATGGGTCATCTGTTGCCTTTCGTGCCTTTTTTGCCCTCTACAATCAAAT TGACCGCTTTAAAAACCATAGCGGTCTTCATACCAATGCCATTTATGGCTTTCATTTGATGCTAGACCACATGATGAAGC GAGTCCAGCCCACACACGTGCTGGTGGCTTTTGATGCGGGCAAAACCACCTTCCGGACAGAAATGTATGCCGATTATAAG GCGGGTCGCGCTAAAACCCCAGAGGAATTTCGTGAGCAGTTTCCTTATATTCGTGAGATGTTGACAGCCCTTGGGATTGC TTATTATGAGTTGGAACACTACGAAGCAGACGATATTATCGGGACGCTTGATAAAATGGCGGAGCGCACAGAAGTGCCTT TTGATGTGACCATTGTCAGTGGTGATAAAGATTTGATCCAGTTAACTGACGAAAATACCGTGGTTGAAATTTCTAAAAAA GGGGTAGCCGAATTTGAAGAATTCACACCGGCTTACCTCATGGAAAAAATGGGATTGACGCCAAACCAGTTTATTGACCT CAAAGCCTTGATGGGAGATAAATCGGATAATATTCCAGGAGTGACCAAAATCGGTGAAAAAACTGGGCTCAAGCTCCTTC ATGAATTTGGTAGTTTAGAAGGTATCTACGAGCATATTGATGGATTCAAGACCTCAAAAATGAAAGAAAACTTGATTAAT GATCGTGACCAAGCTTTCCTATCTAAAACGCTCGCCACTATCAATACAGCATCTCCTATCACAATTGGCTTAGATGATAT TGTCTACAATGGTCCAGATGTTGCTAGTCTCTCTCAATTTTACGATGAGATGGATTTTGTGCAACTCAAGAAAGGTCTTG CTAGCCAGATGCCTCAAGAGCCAGTAGCTGTCATTTCCTATCAAGAAGTGACCAATGTGTCTGCTGATTTGTTTAGCGCA GAAGACATCTTCTACTTTGAAACGCTTCGGGACAATTACCATCGTGAGGCCATCATTGGTTTTGCTTGGGGACATGGTGA GCAGATTTACGCATCCACAGACCTTGGCCTATTAGCGACGGATAGTTTTAAGCAGGTCTTCCAAAAACCCATTGCCACTT ATGATTTTAAACGTAGCAAGGTCTTGTTGAGCCACTTAGGAATTGAGTTGGTAGCACCTAGCTATGATGCTAGATTAGCT AATTACCTTCTGTCAACTGTAGAAGACAATGAGCTTAGCACCATTGCCCGCATTTTTACTGACATCTCTTTGGAAGAAGA CGATACAGTCTATGGTAAAGGTGCTAAACGGGCTGTTCCTGATAAAGACGTCTTACTAGAACACTTAGCTCGTAAGGTCA AAGTATTATTAGATAGCAAGTCTCAAATGCTAGACAAGCTCACTGCCCATGAGCAACTAGACCTCTATCAGAATATTGAA TTGCCTTTGGCTAATGTCCTTGCTAAAATGGAAATTGAAGGGATCAAGGTTAATAGGGCGACTTTGCAAGATATGGCAGA GCAAAACAAGGTCATTATTGAAGCGCTCACCCAAGAGATTTATGATATGGCTGGCCAAGAATTTAATATCAATTCGCCTA AACAGTTAGGCAGTATCTTGTTTGAGAAGATGCAACTACCTCTTGAGATGACTAAGAAGACCAAAACAGGTTATTCTACA GCTGTTAATGTTTTGGAACGTTTGGCACCGATTGCTCCAATTGTGGCTAAAATTTTGGATTACCGCCAGATTACCAAATT ACAGTCTACTTATGTGATTGGTTTACAGGATTATATTCTGGCTGATGGCAAGATTCATACCCGCTATGTGCAGGATCTCA CGCAGACAGGGCGTTTGTCTAGTGTGGACCCTAATCTTCAAAACATTCCCATTCGTTTGGAACAGGGGCGTTTGATTCGT AAGGCCTTTACCCCATCTCATGAAGATGCCGTCTTGTTGAGTTCAGATTACTCACAGATTGAATTGCGGGTACTGGCACA TATTTCAGGGGATGAGCATTTGATTGCTGCCTTTAATGAAGGGGCAGACATTCATACCTCAACGGCTATGCGTGTGTTTG GGATTGACAGGGCAGCAGATGTTACTGCCAATGACCGCCGTAATGCCAAGGCTGTTAACTTTGGAATTGTGTATGGGATT TCTGATTTTGGTTTGTCAAATAACCTTGGTATCACCCGTAAACAGGCCAAGTCTTACATTGATACCTATTTTGAACGCTA TCCTGGGATTAAAGCCTATATGGAAAATGTGGTGCGTGAGGCTAAAGACAAGGGCTATGTAGAGACCCTCTTTAAGCGTC GTCGTGAATTACCGGATATCAACTCTCGTAATTTCAACGTTCGCTCTTTTGCGGAGCGTACAGCCATCAACTCGCCCATT CAAGGAAGCGCAGCTGATATTCTCAAAATCGCTATGATTAATCTGGATAAGGCCTTGCAAGCAGGTGGTTTTAGAGCTAA AATGCTGCTTCAAGTGCATGATGAAATCGTTTTGGAAGTGCCAAATGATGAGTTGACTGCTATAAAAAAACTTGTCAAAG ACACCATGGAAGCTGCTGTTGATTTAGCTGTTCCTTTATGTGTCGATGAAAGTACTGGGCATAGCTGGTACGAAGCCAAA TAA
Upstream 100 bases:
>100_bases TCTAGGAATTATCCTAGGCTTTTTAGGTTAACCATTTACTTATCAGCAAAACCAATGGCTTAAAGGAGAAAAGCCCGTCC CTCTGTGTTATAATAAGCTT
Downstream 100 bases:
>100_bases GTTTTCTTACTTGTTATTTCTCATTTTGACAACTTATGTTATAATAAACTAAAAAATTGTAGGAGAAGTCATGAAGCTGC TTTGAGCTTTTTGACGGATA
Product: DNA polymerase I
Products: NA
Alternate protein names: POL I [H]
Number of amino acids: Translated: 880; Mature: 880
Protein sequence:
>880_residues MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGFHLMLDHMMKRVQPTHVLVAFDAGKTTFRTEMYADYK AGRAKTPEEFREQFPYIREMLTALGIAYYELEHYEADDIIGTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKK GVAEFEEFTPAYLMEKMGLTPNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGSLEGIYEHIDGFKTSKMKENLIN DRDQAFLSKTLATINTASPITIGLDDIVYNGPDVASLSQFYDEMDFVQLKKGLASQMPQEPVAVISYQEVTNVSADLFSA EDIFYFETLRDNYHREAIIGFAWGHGEQIYASTDLGLLATDSFKQVFQKPIATYDFKRSKVLLSHLGIELVAPSYDARLA NYLLSTVEDNELSTIARIFTDISLEEDDTVYGKGAKRAVPDKDVLLEHLARKVKVLLDSKSQMLDKLTAHEQLDLYQNIE LPLANVLAKMEIEGIKVNRATLQDMAEQNKVIIEALTQEIYDMAGQEFNINSPKQLGSILFEKMQLPLEMTKKTKTGYST AVNVLERLAPIAPIVAKILDYRQITKLQSTYVIGLQDYILADGKIHTRYVQDLTQTGRLSSVDPNLQNIPIRLEQGRLIR KAFTPSHEDAVLLSSDYSQIELRVLAHISGDEHLIAAFNEGADIHTSTAMRVFGIDRAADVTANDRRNAKAVNFGIVYGI SDFGLSNNLGITRKQAKSYIDTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDINSRNFNVRSFAERTAINSPI QGSAADILKIAMINLDKALQAGGFRAKMLLQVHDEIVLEVPNDELTAIKKLVKDTMEAAVDLAVPLCVDESTGHSWYEAK
Sequences:
>Translated_880_residues MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGFHLMLDHMMKRVQPTHVLVAFDAGKTTFRTEMYADYK AGRAKTPEEFREQFPYIREMLTALGIAYYELEHYEADDIIGTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKK GVAEFEEFTPAYLMEKMGLTPNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGSLEGIYEHIDGFKTSKMKENLIN DRDQAFLSKTLATINTASPITIGLDDIVYNGPDVASLSQFYDEMDFVQLKKGLASQMPQEPVAVISYQEVTNVSADLFSA EDIFYFETLRDNYHREAIIGFAWGHGEQIYASTDLGLLATDSFKQVFQKPIATYDFKRSKVLLSHLGIELVAPSYDARLA NYLLSTVEDNELSTIARIFTDISLEEDDTVYGKGAKRAVPDKDVLLEHLARKVKVLLDSKSQMLDKLTAHEQLDLYQNIE LPLANVLAKMEIEGIKVNRATLQDMAEQNKVIIEALTQEIYDMAGQEFNINSPKQLGSILFEKMQLPLEMTKKTKTGYST AVNVLERLAPIAPIVAKILDYRQITKLQSTYVIGLQDYILADGKIHTRYVQDLTQTGRLSSVDPNLQNIPIRLEQGRLIR KAFTPSHEDAVLLSSDYSQIELRVLAHISGDEHLIAAFNEGADIHTSTAMRVFGIDRAADVTANDRRNAKAVNFGIVYGI SDFGLSNNLGITRKQAKSYIDTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDINSRNFNVRSFAERTAINSPI QGSAADILKIAMINLDKALQAGGFRAKMLLQVHDEIVLEVPNDELTAIKKLVKDTMEAAVDLAVPLCVDESTGHSWYEAK >Mature_880_residues MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGFHLMLDHMMKRVQPTHVLVAFDAGKTTFRTEMYADYK AGRAKTPEEFREQFPYIREMLTALGIAYYELEHYEADDIIGTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKK GVAEFEEFTPAYLMEKMGLTPNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGSLEGIYEHIDGFKTSKMKENLIN DRDQAFLSKTLATINTASPITIGLDDIVYNGPDVASLSQFYDEMDFVQLKKGLASQMPQEPVAVISYQEVTNVSADLFSA EDIFYFETLRDNYHREAIIGFAWGHGEQIYASTDLGLLATDSFKQVFQKPIATYDFKRSKVLLSHLGIELVAPSYDARLA NYLLSTVEDNELSTIARIFTDISLEEDDTVYGKGAKRAVPDKDVLLEHLARKVKVLLDSKSQMLDKLTAHEQLDLYQNIE LPLANVLAKMEIEGIKVNRATLQDMAEQNKVIIEALTQEIYDMAGQEFNINSPKQLGSILFEKMQLPLEMTKKTKTGYST AVNVLERLAPIAPIVAKILDYRQITKLQSTYVIGLQDYILADGKIHTRYVQDLTQTGRLSSVDPNLQNIPIRLEQGRLIR KAFTPSHEDAVLLSSDYSQIELRVLAHISGDEHLIAAFNEGADIHTSTAMRVFGIDRAADVTANDRRNAKAVNFGIVYGI SDFGLSNNLGITRKQAKSYIDTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDINSRNFNVRSFAERTAINSPI QGSAADILKIAMINLDKALQAGGFRAKMLLQVHDEIVLEVPNDELTAIKKLVKDTMEAAVDLAVPLCVDESTGHSWYEAK
Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]
COG id: COG0749
COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 5'-3' exonuclease domain [H]
Homologues:
Organism=Homo sapiens, GI112420958, Length=478, Percent_Identity=31.7991631799163, Blast_Score=223, Evalue=5e-58, Organism=Homo sapiens, GI139394648, Length=699, Percent_Identity=26.89556509299, Blast_Score=214, Evalue=3e-55, Organism=Escherichia coli, GI1790294, Length=941, Percent_Identity=37.8320935175345, Blast_Score=529, Evalue=1e-151, Organism=Escherichia coli, GI226510970, Length=211, Percent_Identity=32.2274881516588, Blast_Score=114, Evalue=3e-26, Organism=Caenorhabditis elegans, GI86563272, Length=393, Percent_Identity=29.2620865139949, Blast_Score=170, Evalue=3e-42, Organism=Drosophila melanogaster, GI17933644, Length=439, Percent_Identity=32.5740318906606, Blast_Score=229, Evalue=9e-60,
Paralogues:
None
Copy number: 400 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002562 - InterPro: IPR020046 - InterPro: IPR020045 - InterPro: IPR002421 - InterPro: IPR020047 - InterPro: IPR019760 - InterPro: IPR001098 - InterPro: IPR018320 - InterPro: IPR002298 - InterPro: IPR008918 - InterPro: IPR012337 [H]
Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 99026; Mature: 99026
Theoretical pI: Translated: 5.14; Mature: 5.14
Prosite motif: PS00447 DNA_POLYMERASE_A
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGFHLMLDHMMKRVQPTHV CCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE LVAFDAGKTTFRTEMYADYKAGRAKTPEEFREQFPYIREMLTALGIAYYELEHYEADDII EEEEECCCCEEEHHHHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHH GTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKKGVAEFEEFTPAYLMEKMGLT HHHHHHHHHCCCCEEEEEEECCCCEEEECCCCEEEEECCCCHHHHHHCCHHHHHHHHCCC PNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGSLEGIYEHIDGFKTSKMKENLIN CCHHEEHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHC DRDQAFLSKTLATINTASPITIGLDDIVYNGPDVASLSQFYDEMDFVQLKKGLASQMPQE CHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC PVAVISYQEVTNVSADLFSAEDIFYFETLRDNYHREAIIGFAWGHGEQIYASTDLGLLAT CEEEEEHHHHHCCHHHHCCCCHHHHHHHHHCCCCCEEEEEEEECCCCEEEEECCCCEEEC DSFKQVFQKPIATYDFKRSKVLLSHLGIELVAPSYDARLANYLLSTVEDNELSTIARIFT HHHHHHHHCCHHHCCCHHHHHHHHHCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHH DISLEEDDTVYGKGAKRAVPDKDVLLEHLARKVKVLLDSKSQMLDKLTAHEQLDLYQNIE HCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC LPLANVLAKMEIEGIKVNRATLQDMAEQNKVIIEALTQEIYDMAGQEFNINSPKQLGSIL CCHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH FEKMQLPLEMTKKTKTGYSTAVNVLERLAPIAPIVAKILDYRQITKLQSTYVIGLQDYIL HHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHEE ADGKIHTRYVQDLTQTGRLSSVDPNLQNIPIRLEQGRLIRKAFTPSHEDAVLLSSDYSQI ECCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHHHCCCCCCCEEEEECCCCCE ELRVLAHISGDEHLIAAFNEGADIHTSTAMRVFGIDRAADVTANDRRNAKAVNFGIVYGI EEEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEEEE SDFGLSNNLGITRKQAKSYIDTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDI CCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC NSRNFNVRSFAERTAINSPIQGSAADILKIAMINLDKALQAGGFRAKMLLQVHDEIVLEV CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEC PNDELTAIKKLVKDTMEAAVDLAVPLCVDESTGHSWYEAK CCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCC >Mature Secondary Structure MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGFHLMLDHMMKRVQPTHV CCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE LVAFDAGKTTFRTEMYADYKAGRAKTPEEFREQFPYIREMLTALGIAYYELEHYEADDII EEEEECCCCEEEHHHHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHH GTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKKGVAEFEEFTPAYLMEKMGLT HHHHHHHHHCCCCEEEEEEECCCCEEEECCCCEEEEECCCCHHHHHHCCHHHHHHHHCCC PNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGSLEGIYEHIDGFKTSKMKENLIN CCHHEEHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHC DRDQAFLSKTLATINTASPITIGLDDIVYNGPDVASLSQFYDEMDFVQLKKGLASQMPQE CHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC PVAVISYQEVTNVSADLFSAEDIFYFETLRDNYHREAIIGFAWGHGEQIYASTDLGLLAT CEEEEEHHHHHCCHHHHCCCCHHHHHHHHHCCCCCEEEEEEEECCCCEEEEECCCCEEEC DSFKQVFQKPIATYDFKRSKVLLSHLGIELVAPSYDARLANYLLSTVEDNELSTIARIFT HHHHHHHHCCHHHCCCHHHHHHHHHCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHH DISLEEDDTVYGKGAKRAVPDKDVLLEHLARKVKVLLDSKSQMLDKLTAHEQLDLYQNIE HCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC LPLANVLAKMEIEGIKVNRATLQDMAEQNKVIIEALTQEIYDMAGQEFNINSPKQLGSIL CCHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH FEKMQLPLEMTKKTKTGYSTAVNVLERLAPIAPIVAKILDYRQITKLQSTYVIGLQDYIL HHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHEE ADGKIHTRYVQDLTQTGRLSSVDPNLQNIPIRLEQGRLIRKAFTPSHEDAVLLSSDYSQI ECCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHHHCCCCCCCEEEEECCCCCE ELRVLAHISGDEHLIAAFNEGADIHTSTAMRVFGIDRAADVTANDRRNAKAVNFGIVYGI EEEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEEEE SDFGLSNNLGITRKQAKSYIDTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDI CCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC NSRNFNVRSFAERTAINSPIQGSAADILKIAMINLDKALQAGGFRAKMLLQVHDEIVLEV CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEC PNDELTAIKKLVKDTMEAAVDLAVPLCVDESTGHSWYEAK CCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11463916 [H]